Takara_双酶切buffer用表

EnzymeAccIBamHIBglIIClaIEcoRIEcoRVHincIIHindIIIKpnINcoINdeINotIPstIPvuISacISalISmaISpeISphIXbaIXhoISuppliedBuffer10×M10×K10×H10×M10×H10×H10×M10×M10×L10×K+BSA10×H10×H+BSA+Triton10×H10×K+BSA10×L10×H10×T+BSA10×M10×H10×M+BSA10×HAccI-0.5×K1×T1×M1×M0.5×K1×M1×M1×M1×M+BSA1×T0.5×K+BSA1×M0.5×K1×M1.5×T1×T+BSA1×M0.5×K1×M1×MBamHI0.5×K-1×K1×K1×K1×K0.5×K1×K0.5×K1×K+BSA1×K0.5×K+BSA1×K1×K0.5×K1.5×T0.5×T+BSA1×K1×K0.5×K1×KBglII1×T1×K-1×H1×H1×H2×K1×K1×T1×K+BSA1×H1×H+BSA1×H1×K0.5×K1×H1×T+BSA1×H1×H2×T1×HClaI1×M1×K1×H-1×H1×H1×M1×M1×M1×K+BSA1×H1×H+BSA1×H1×K1×M1×H1×T+BSA1×M1×H1×M1×HEcoRI1×M1×K1×H1×H-1×H1×M1×M1×M1×K+BSA1×H1×H+BSA1×H1×K1×M1×H1×T+BSA1×H1×H1×M1×HEcoRV0.5×K1×K1×H1×H1×H-2×T1×K0.5×K1×K+BSA1×H1×H+BSA1×H1×K0.5×K1×H0.5×K+BSA1×H1×H2×T1×HHincII1×M0.5×K2×K1×M1×M2×T-1×M1×M1×M+BSA1×T0.5×K+BSA1×M0.5×K1×M1.5×K1×T+BSA1×M2×T1×M1×MHindIII1×M1×K1×K1×M1×M1×K1×M-1×M1×K+BSA1×K0.5×K+BSA1×M1×K1×M1.5×K1×T+BSA1×M1×K1×M1×MKpnI1×M0.5×K1×T1×M1×M0.5×K1×M1×M-0.5×K+BSA1×T0.5×K+BSA1×M0.5×K1×L1.5×T+BSA1×T+BSA1×M0.5×K1×M1×MNcoI1×M+BSA1×K+BSA1×K+BSA1×K+BSA1×K+BSA1×K+BSA1×M+BSA1×K+BSA0.5×K+BSA-1×K+BSA0.5×K+BSA1×K+BSA1×K+BSA0.5×K+BSA1.5×T+BSA1×T+BSA1×K+BSA1×K+BSA1×M+BSA1×K+BSANdeI1×T1×K1×H1×H1×H1×H1×T1×K1×T1×K+BSA-1×H+BSA1×H1×K1×T1×H1×T+BSA1×H1×H1×T1×HNotI0.5×K+BSA0.5×K+BSA1×H+BSA1×H+BSA1×H+BSA1×H+BSA0.5×K+BSA0.5×K+BSA0.5×K+BSA0.5×K+BSA1×H+BSA-1×H+BSA2×K+BSA0.5×K+BSA1×H+BSA0.5×T+BSA1×H+BSA1×H+BSA0.5×K+BSA1×H+BSAPstI1×M1×K1×H1×H1×H1×H1×M1×M1×M1×K+BSA1×H1×H+BSA-1×K1×M1×H0.5×T+BSA1×H1×H1×M1×HPvuI0.5×K1×K1×K1×K1×K1×K0.5×K1×K0.5×K1×K+BSA1×K2×K+BSA1×K-0.5×K1.5×K+BSA1×K+BSA1×K1×K0.5×K1×KSacI1×M0.5×K0.5×K1×M1×M0.5×K1×M1×M1×L0.5×K+BSA1×T0.5×K+BSA1×M0.5×K-1.5×T+BSA1×T+BSA1×M0.5×K1×M1×MSalI1.5×T1.5×T1×H1×H1×H1×H1.5×K1.5×K1.5×T+BSA1.5×T+BSA1×H1×H+BSA1×H1.5×K+BSA1.5×T+BSA-1.5×T+BSA1×H1×H1.5×T1×HSmaI1×T+BSA0.5×T+BSA1×T+BSA1×T+BSA1×T+BSA0.5×K+BSA1×T+BSA1×T+BSA1×T+BSA1×T+BSA1×T+BSA0.5×T+BSA0.5×T+BSA1×K+BSA1×T+BSA1.5×T+BSA-1×T+BSA0.5×T+BSA1×T+BSA1×T+BSASpeI1×M1×K1×H1×M1×H1×H1×M1×M1×M1×K+BSA1×H1×H+BSA1×H1×K1×M1×H1×T+BSA-1×H1×M1×HSphI0.5×K1×K1×H1×H1×H1×H2×T1×K0.5×K1×K+BSA1×H1×H+BSA1×H1×K0.5×K1×H0.5×T+BSA1×H-2×T1×HXbaI1×M0.5×K2×T1×M1×M2×T1×M1×M1×M1×M+BSA1×T0.5×K+BSA1×M0.5×K1×M1.5×T1×T+BSA1×M2×T-1×MXhoI1×M1×K1×H1×H1×H1×H1×M1×M1×M1×K+BSA1×H1×H+BSA1×H1×K1×M1×H1×T+BSA1×H1×H1×M-Note:1)Itisconfirmedthat10unitsofeachenzymecompletelydigest1µgofDNAat37°Cinonehourin50µlreactionmixture.2)TheconcentrationofGlycerolshouldbelessthan10%tominimizestaractivity.3)DNAmaynotbedigestedcompletely,whenrecognitionsequencesoftwoenzymesarecloseeachother,orwhenDNAtakeshigh-structureconformation.Relativeactivityforeachreactionbuffer:supplied,activitymeasuredbuffer:recommendedbufferRestrictionenzymeRelativeactivities(%)LMHKT+BSABasal***AatII20202020100120AccI2010020(20)16080AccII(260)1002020200160AccIII(20)(20)20(80)(20)100AfaI60604060100100AflII2080*2020140120AluI1001002040200120Aor13HI20202080*80100Aor51HI801002020120120ApaI10020202020120ApaLI100202020120120AvaI(20)1002040100120AvaII801002020100100BalI2020202040100BamHI(20)2040100(20)80BanII(120)(120)10080(100)100BbeI2020202020100BcnI2020406060100BglI2020204020100BglII2020100(100)(60)100BlnI20204010020120BmeT110I20202010020140BmgT120I20201004020240Bpu1102I2020204060100BspT104I100602020100120BspT107I20208010020100Bsp1286I10020202060100Bsp1407I20602020100100BssHII1001006020140100BstPI(20)(60)100(100)(100)100BstXI20401002020120Bst1107I(20)6010010040100Cfr10I(20)(20)(20)40(20)100ClaI4010012010060100CpoI20208010020100DraI100100601008080EaeI601002020120160Eam1105I(20)(40)2040(40)100EcoO65I(20)(60)60*4040100EcoO109I100602020100160EcoRI(20)(100)100(120)(80)120EcoRV(20)(40)100(120)(40)100EcoT14I(20)(40)100120(60)100EcoT22I2020100(140)(20)120Eco52I2020202020100Eco81I201002020100160FbaI(20)(20)(80)100(20)100FokI(20)(60)2020(200)100HaeII801002080140100HaeIII6010010060100100HapII10060202010080HhaI80100100120120100HincII20100204010080HindIII(60)10020200(100)80HinfI8010010016060100Hin1I4080*202060160HpaI20(40)20100(80)100KpnI100602020(100)80MboI20406010040100MboII10060202060100MflI10080202080100MluI6060100(100)60100MspI80802010010080MunI(200)100*2020160100MvaI(20)(40)80100(20)120NaeI100202020100120NcoI(40)(60)2060*(60)160NdeI204010010080100NheI(120)1002020(160)100NotI(20)(20)20**20(20)100NruI020202020100NsbI40202060100100PmaCI100802020100100PshAI20402010060160PshBI(20)(40)204040100Psp1406I20602020100100PstI(20)(60)10080(20)80PvuI(20)(20)(40)80*(40)120PvuII(80)1004020(40)100SacI1006020208080SacII4020202010040SalI2020100(20)20120Sau3AI(60)8010020(80)100ScaI(20)(20)100(60)(20)100SfiI(40)1002020100100SmaI20202020100100SmiI10201004010100SnaBI(20)(40)2020(40)100SpeI(80)10080100(80)100SphI(20)(40)100120(20)100Sse8387I(120)60*2020(60)100SspI(20)(60)40(100)(80)100StuI601006080140100TaqI4080606080100Tth111I(20)8040100(80)120Van91I20(20)60100(60)100VpaK11BI202060(40)20100XbaI2080*2020120120XhoI206010016060100XspI2060201001