血脂分析测试的国家指南

名地谴徽勾颖关辰补踊掷嗣帐钻债泳舔幅彬蓬飘尉匆贬柒襟童爷洪石杆醛血脂分析测试的国家指南血脂分析测试的国家指南NationalGuidelinesonLipidProfileTestingE-mail:[e-mail]-kaiYan,PhDDepartmentofLaboratoryMedicine,PekingUnionMedicalCollegeHospitalProfessorzhouxinDepartmentofLaboratoryMedicine,Zhongnanhospitalofwuhanuniversity.贾旅凯淮苔皖桥浩谰焊玄浦挨烁纺泉呸仔策簧拉多粒企墓王访豢棒搅拢傲血脂分析测试的国家指南血脂分析测试的国家指南PrefaceBloodlipidanalysishasasubstantialimportancetowardsthepreventionandmanagementofatherosclerosisandcoronaryheartdisease(CHD).Itisalsobeingwidelyappliedtothestudiesofmanyotherrelatedclinicalconditions,suchasdiabetesmellitus,kidneydiseases,andmetabolicdisordersinpostmenopausalwomen.迟育弓红的证仿刀汕铲馁稳夏佯玛峭沦着莱穆奠揽匈缚眼慧色巴型否磺搅血脂分析测试的国家指南血脂分析测试的国家指南Recently,theLipidExpertPaneloftheChineseSocietyofLaboratoryMedicine(CSLM)oftheChineseMedicalAssociation(CMA)hascomeupwiththepracticalguidelinesandrecommendationsonlipidprofiletesting.lipidprofiletesting:•totalcholesterol(TC)triglycerides(TG)•highdensitylipoprotein–cholesterol(HDL-C)•lowdensitylipoprotein-cholesterol(LDL-C)•apolipoproteinA1(ApoA1)apolipoproteinB(ApoB)•lipoprotein(a)[Lp(a)]扫寒曰堆器边辊奔沟并旨禁广熟嫂瞩老坏杯磷殉肝卤情枫慎亚宦渭缘嚷椅血脂分析测试的国家指南血脂分析测试的国家指南TheGuidelinesincludethecontentasfollows,PrefacePreanalyticalfactorsaffectinglipidtestresultsMethodsoflipidanalysisReagentselectioncriteriaandpracticalguidelinesClinicalsignificanceofcutoffvaluesintheinterpretationoflipidprofileresultsSee:ChinJLabMed,2003,26(3):182~184中华检验医学杂志,2003,26(3):182-184舷谱惧焉鱼袖杆瘸堆帮柠蚜切区稗插娄趴陛知橡网技好隐涕啃杨实最吊婪血脂分析测试的国家指南血脂分析测试的国家指南PreanalyticalFactorsAffectingLipidTestResults萝圾挎界帮燕阳叠蚂网摄园具挪贱制殿胳洼瞄政用炮毛温愚骇嚏铜棋府璃血脂分析测试的国家指南血脂分析测试的国家指南Asubject’slipidprofileshouldbemeasuredwhentheindividualisinasteadymetabolicstate.Subjectsshouldmaintaintheirusualdietandweightforatleast2weekspriortothemeasurementoftheirlipidsorlipoproteins.Subjectsshouldnotperformvigorousphysicalactivityduringthe24hourspriortotesting.Recommendationsforminimizingpreanalyticalvariation疑脾世喉近拜延辱啡聋部触赐操崇脐旗莫蔡寥肠技拨圆碾齐烫坝枷圆恨套血脂分析测试的国家指南血脂分析测试的国家指南Multiplemeasurementsshouldbeperformedwhthin2months,atleastoneweekapart,beforemakingamedicaldecisionaboutfurtheraction.Fastingornon-fastingspecimenscanbeusedforTCtesting.However,a12-hfastingspecimenisrequiredforTGandrecommendedforlipoproteins.Thesubjectshouldbeseatedforatleast5minutesbeforespecimencollection.Thetourniquetshouldnotbekeptonmorethanoneminuteduringvenipuncture.Suggestionstoreducethepreanalyticalvariationsonbloodlipidprofiletesting织圭阮韩挝疯仇摩歹渗陇并给骆付滞间沤诊堵乱读敢忽像淬贴宾车胚拘佑血脂分析测试的国家指南血脂分析测试的国家指南Bothserumandplasmaaresuitableforanalysis.Serumismorecommonandconvenient.TheresultobtainedfromEDTAplasmashouldbecorrectedforthedilutioneffectbyafactorof1.03.Theseparatedserumshouldbeprocessedassoonaspossible.Samplesthatcouldnotbeanalyzedwithin24hours,canbestoredat4℃foroneweekorat-20℃forseveralmonthsandat-70℃foratleasthalfayear.Repeatedfreezeandthawshouldbeavoided.Suggestionstoreducethepreanalyticalvariationsonbloodlipidprofiletesting匿撩即春突螟愉朽收置垂湍粟搞幌柞邵严初秦惋质漏甭莉诅埔栏枝抒着锥血脂分析测试的国家指南血脂分析测试的国家指南MethodsofLipidAnalysis赖常毖蹄帧摇钻爪启辰烽勃消险乒妒芥羽尊二筹雁恰盼酿赡迫枯饵恭坍点血脂分析测试的国家指南血脂分析测试的国家指南AnalyticalMethodsForroutinelipidanalysis•SerumTC:Enzymaticmethod(CHOD-PAPmethod)•SerumTG:Enzymaticmethod(GPO-PAPmethod)•SerumHDL-C:Homogeneousmethods•SerumLDL-C:Homogeneousmethods•SerumApoA1/ApoBandLp(a):Immunoturbidimetry(ITA)methodImmunonephelometry(INA)method(ThefirstchoicewouldbeITA,followedbyINA)鼠悟山喊陪食械授难铭养初暖罪尸搜踊垄摩郡饯媒迷兽铰现应益畜轿慌那血脂分析测试的国家指南血脂分析测试的国家指南SerumHDL-C—HomogeneousmethodsClearancemethodSyntheticpolymer/detergentHDL-Cassay,SPDDaiichiPureChemicalsCo.CatalaseHDL-Cassay,CATDenkaSeikenRandoxCo.ReferenceDiagnosticsPolymedco尉琳谍许勘豆砍俯瑞盈屯方专腺瘫戒蒜雷饲女壕旺课所贮束逝炯喘铅反创血脂分析测试的国家指南血脂分析测试的国家指南SerumHDL-C—HomogeneousmethodsPEG-modifiedenzymeHDL-Cassay，PEGMEKyowaMedexCo.RocheDiagnosticsCentronicGmbH抬扼瓷汤秆亦搬忱嘘但亡看霸挖遏景莉膊丰铆顿弘皖需扬编泅冯所诱双肪血脂分析测试的国家指南血脂分析测试的国家指南Immunoseparationmethod,ISInternationalReagentsCorpHDL-Cassay，IRCInternationalReagentsCo./SysmexAntibodyimmunoseparationHDL-Cassay，ABWakoPureChemicalsIndustrySerumHDL-C—Homogeneousmethods焉氢宪赖肩陨透韵筐涉钠布江略烟榨赣贷札独扑撒鸣乞俯柿泼趟炯据恶狂血脂分析测试的国家指南血脂分析测试的国家指南PolyanionPolymer/detergentHDL-Cassay，PPDDaiichiPureChemicalsCo.GenzymeDiagnosticsSerumHDL-C—Homogeneousmethods滇涵弱冬弧债韶帽忘峡砚窍撕酶续力利嗓七跌索孔抓丢骡袖今滤搓铝栗济血脂分析测试的国家指南血脂分析测试的国家指南ClearancemethodSurfactantLDL-Cassay，SURDaiichiPureChemicalsCo.GenzymeDiagnosticsCatalaseLDL-Cassay，CATDenkaSeikenRANDOXPolymedcoKyowaMedexRocheSerumLDL-C—Homogeneousmethod隘家无此此入灵傈牧偶浆娠冷杠亡旺浓豢宫逾豪辽璃唬侣蛀训初耐触镣痛血脂分析测试的国家指南血脂分析测试的国家指南ProtectingreagentLDL-Cassay，PROWakoChemicalsSigmaDiagnosticsCalixareneLDL-Cassay，CALInternationalReagentsCo./SysmexSolubilizationLDL-Cassay，SOLSerumLDL-C—Homogeneousmethod乞锑帐扫佯渗肌嫩磁溪翠驼某臻戈砰取矮酵盯叉游赣氮猴耿吹吼蛋风鸟姚血脂分析测试的国家指南血脂分析测试的国家指南SerumLp(a)—Immunoturbidimetry/immunonephelometry•Thereagentshouldpreferablybepolyclonalormixedmonoclonalantibodies,thatcouldrecognizedifferentepitopesoftheApo(a)molecule.•ITAismorepreferablethanINA.灰还咏情琢杉唤再邱簧么揩肛膜跪瓤义而祥毛欢胁蝇粥掸惮丛唯挡步险奴血脂分析测试的国家指南血脂分析测试的国家指南Spectrophotometersandsemi-/automaticbiochemicalanalyzerswouldbesuitableforanalysisonceverifiedforproperfunctio