western-blot-配胶表

5%分离胶6%分离胶7%分离胶8%分离胶超纯水5.6ml5.3ml5.0ml4.6ml30%丙烯酰胺1.7ml2.0ml2.3ml2.7ml1.5mol/LTrisHcl(PH8.8)2.5ml2.5ml2.5ml2.5ml0.5mol/LTrisHcl(PH6.8)10%SDS100ul100ul100ul100ul10%过硫酸铵100ul100ul100ul100ulTEMED10ul10ul10ul10ul最佳分离蛋白的分子量（kDa)60-21250-15030-12030-9010%分离胶12%分离胶112%分离胶15%分离胶4%浓缩胶5浓缩胶4.0ml3.3ml2.3ml4.3ml3.4ml3.3ml4.0ml5.0ml0.7ml0.83ml2.5ml2.5ml2.5ml0.63ml0.63ml100ul100ul100ul50ul50ul100ul100ul100ul50ul50ul5ul5ul5ul10ul10ul20-8012-6010-40