超声波法从葡萄籽中提取原花青素

22120051精细化工FINECHEMICALSVol.22,No.1Jan.2005钟振声,冯　焱,孙立杰(,　510640):,,。,0.6～1mm,1g3mL,48h;1g20mL,〔w(C2H5OH)=95%〕、〔w(CH3COCH3)=99%〕2h,,4.63%、4.59%2.55%(),11%、66%48%。:;;;:TS201.1　　:A　　:1003-5214(2005)01-0041-03ExtractionofProanthocyanidinsfromGrapeSeedswithUltrasonicWaveZHONGZhen-sheng,FENGYan,SUNLi-jie(SchoolofChemicalScience,SouthChinaUniversityofTechnology,Guangzhou510640,Guangdong,China)Abstract:Ultrasonicwavewasappliedtoextractionofproanthocyanidinsfromgrapeseeds.Comparedwithtraditionalsolventextractionmethod,theeffectsofultrasonicwaveonextractionefficiencyandproductconcentrationwerestudied.Thedriedgrapeseedswerecrusheddownto0.6～1mmandsoakedinfirstsolvent,intheratioof1ggrapeseedsto3mLpetroleumether,atroomtemperaturefor48hourstoremovethelipid,thensoakedwithultrasonicwaveatroomtemperaturefor2hoursinthesecondsolvent,intheratioof1ggrapeseedsto20mLsecondsolvent.Thesecondsolventswereethanol〔w(C2H5OH)=95%〕,acetone〔w(CH3COCH3)=99%〕andpurewater.Theresultsshowedthattheyieldswererespectively4.63%,4.59%and2.55%basedontheweightofgrapeseedsused,andtheextractionefficiencieswererespectively11%,66%and48%higherthanthatoftheprocesswithoutultrasonicwave.Keywords:proanthocyanidins;grapeseeds;extracting;ultrasonicwave　　、,,,、。,w()≥5%[1]。,,,。,,,。,,,、。,,、、,。,、、、,,,,[2]。:2004-06-02;:2004-07-16:(1955-),,,,、、,:020-87112906,E-mail:[e-mail]。,。,、、、,。1　1.1　〔w(C2H5OH)=95%〕,〔w(CH3COCH3)=99%〕,(30～60℃)〔w()=99%〕AR。,,,,20～25℃,。1.2　JB-2;180L(240W);ZFQ31;SHZ-D;ZK-82A;754。1.3　,,,,。、、,、、、[3]。,,,、、。,。1.4　18,30,18～30(0.6～1mm)。10g,30mL,48h,。,。500mL,200mL、,20～25℃2h,。,3,500mL,,,。[4],。2　2.1　,。,。,3,。11。:/%=(/)×100w()/%=(/)×100表1　室温下超声波法与溶剂法提取原花青素的提取结果比较Table1　Comparisonoftheyieldsofproanthocyanidinextractionwithorwithoutultrasonicwaveat25℃/%w()/%4.634.592.5552.7459.8729.684.162.761.7250.0356.4626.951,,、,11%、66%48%(1)。,,。图1　两种方法提取原花青素的提取率比较Fig.1　Comparisonoftheyieldsofproanthocyanidinextractionwithorwithoutultrasonicwave2.2　50℃,50℃2.1。2。表2　50℃下超声波法与溶剂法原花青素提取率比较表Table2　Comparisonoftheyieldsofproanthocyanidinextractionwithorwithoutultrasonicwaveat50℃/%w()/%4.704.682.5652.8660.1329.804.934.813.3956.4765.7130.94,50℃,,。。1、2,,,。2.3　、。、、、,·42·精细化工　FINECHEMICALS　　　　　　　　　　　　　　22　,。,,,。,,。,。50℃。,,,,。,,。,,。2.4　3,,,,,。。[5]2。图2　原花青素结构式Fig.2　Structureofproanthocyanidins2,;,。,,。,,,,。,,,,。。3　(1),4.6%,11%66%。2.5%,48%。。(2),25℃50℃,。,。(3)。,,,。(4),,,,,。:[1]　Shrikhande,AnilJ.Wineby-productswithhealthbenefits[J].FoodResearchInternational,2000,33(6):469-474.[2]　,,.[J].,2001,23(5):289-291.[3]　,　,.[J].,2001,18(7):394-395,400.[4]　.[J].,2002,23(6):90-92.[5]　,　.[J].,2001,20(2):208-210.———4.5%,、、、。,4.5%,,700kg,,,,,,“”。,,,,,。:,;:300071;:022-23508838·43·1,: