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-1-31DNADNADNA1.12DNA3DNA2.DNA(1)DNAEcoliDNAT4-DNAEcoliDNAT4DNAT4DNA(2)DNA:DNADNADNA3.1DNADNA2,DNA3()1.2.__3.PCR1DNA29095DNA5560DNA7075DNA1.2.1DNARNAmRNA2DNA-2-3_1.2.Ca2+DNADNA3.1.DNADNA2.mRNAmRNA3.4.1.2.3.21.2.12-3-33.12PEG31.123436.50.5pH7.27.495%5%CO2O2CO2pH52.12()()()-4-3453.1234-5-4.1B23453121233245B-6-1ESEK23ESESES1.(1)(2)(3)(4)(4)2.(1)(2)(3)(4)-7-7()1963.(1)24(2)(3)(4)4(1)(2)(3)(1)(2)(3)(1)(2)(3)-8-(4)512[]3[][][][][]123456