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122520080617JournalofClinicalRehabilitativeTissueEngineeringResearchJune17,2008Vol.12,No.25ISSN1673-8225CN21-1539/RCODEN:ZLKHAH4927121ResearchprogressinhumanamnioticepithelialcellsSongXiu-jun1,ChenDai-xiong2,JiangQi-sheng1AbstractHumanamnioticepithelialcellsarederivedfromplacentalamnioticmembrane.Theyexpressstemcellmarkersandrelatedgene,butnottelomerasegene.HumanamnioticepithelialcellsalsoappeartoexpresslowlevelofMHC-IandnottoexpressMHC-IIsothatimmunologicalrejectiondoesnottakeplaceafterallograft.Inaddition,theycansecreteimmunosuppressionfactorsthatcanpreventinflammatoryreactionpostgraft.Humanamnioticepithelialcellshaveobviousplasticityandcapacityofmulti-directionaldifferentiation.Undertheregulationofdifferentgrowthfactors,theycandifferentiateintomanykindsofcells,suchashepatocyte-likecells,cardiomyocyte-likecells,neuroglialcells,nervecells,pancreaticislet-likecellsandsoon.Thushumanamnioticepithelialcellsarepromisingincellsubstitutetreatmentandtissueregenerationmedicine.However,wecannotcallit“stemcell”,becauseitlacksofthecapacityoflong-termself-renewalandproductionofsinglecellcolony.Additionally,theclassificationoffunctionalcellsdifferentiatedfromhumanamnioticepithelialcellsinvivoneedsfurthervalidation.SongXJ,ChenDX,JiangQS.Researchprogressinhumanamnioticepithelialcells.ZhongguoZuzhiGongchengYanjiuyuLinchuangKangfu2008;12(25):4927-4930(China)[(ps).pdf].[J].200812(25):4927-4930[(ps).pdf]32.11990~2007amnioticmembrane,humanamnioticepithelialcellPubmed://[1-12]20[13-32]1CentralLaboratoryofGeneralHospitalofSecondArtilleryForcesofChinesePLA,Beijing100088,China;2KeyLabora-toryofCellEngineer-ingofGuizhouProv-ince,Zunyi563003,GuizhouProvince,ChinaSongXiu-jun,Master,Technician,CentralLaboratoryofGeneralHospitalofSecondArtilleryForcesofChinesePLA,Beijing100088,Chinasongxiujun1979@163.comReceived:2007-12-01Accepted:2008-02-221100088256300319792007songxiujun1979@163.com:R329.2:A:1673-8225(2008)25-04927-042007-12-012008-02-22(07-50-12-6692/ZS·A),.P.O.Box1200,Shenyang110004kf23385083@sina.com4928~0.05mm5I2m22[1-2]4.1.2SSEA-3SSEA-4TRA-1-60TRA-1-81SOX-2FGF-4Rex-1c-kitThy-1[3-4]Oct-4NanogDNA[3]CD44CD73CD90(Thy1)CD105[5]4.1.3MHC-1[6]Adinolfi[7]HLA-ABCDR2-IIISakuragawa[8]MHC-IIMHC-IMHC-IMHC-II-(100U/mL)3dTerada[9-10]HLA-A/BCDR[11-12]4.24.2.1[13][14][15][16]Kakishita,.ISSN1673-8225CN21-1539/RCODEN:ZLKHAH4929[17]RT-PCR1ATCK18GSCPS-IPEPCKCYP2D6CYP3A42AFPTTRTATCYP2C9OTC-6-TDO[18]Miki[3]1-EGF4.2.2Sakuragawa[19-20]/3IF-1IF-4IF-6[21]2[20-21]Elwan[22](DA)D2mRNART-PCR(DA)D2mRNADAD2100%Miki[3]Ilancheran[23]Knofler[24]bHLHHandImRNAbHLHKakishita[25]6-SankarKoji[26-27][28][29][30]NSCs4.2.3Miki[3]RT-PCRPDX-1mRNA6(pairedboxhomeoticgene6,Pax-6)2(locus2,Nkx2.2)[31]Miki[3]14d2(atrialandventricularmyosinlightchain2,MLC-2A,MLC-2V)GATA-4Nkx2.5-Kakishita[25]7~1434~40[23,31]Wolbank[32]Ilancheran[23]1,.P.O.Box1200,Shenyang110004kf23385083@sina.com4930[3],1.[J].200610(1):79-812[J].200711(32):6450-64533MikiT,LehmannT,HongboC,etal.StemCellCharacteristicsofAmnioticEpithelialCells.StemCells200523(10):1549-15594MikiT,StromSC.Amnion-derivedpluripotent/multipotentstemcells.Stemcell200621331425HeegerPS.AmnionandChorionCellsasTherapeuticAgentsforTransplantationandTissueRegeneration:AFieldinItsInfancy.Transplantation200478(10):1411-14126AkleCA,AdinolfiM,WelshKI,etal.Immunogenicityofhumanamnioticepithelialcellsaftertransplantationintovolunteers.Lancet19812(8254):1003-10057AdinolfiM,AkleCA,MccollI,etal.ExpressionofHLAantigens,beta2-microglobulinandenzymesbyhumanamnioticepithelialcells.Nature1982295(5847):325-3278SakuragawaN,TohyamaJ,YamamotoH.Immunostainingofhumanamnioticepithelialcells:possibleuseasatransgenecarrieringenetherapyforinbornerrorsofmetabolism.CellTransplant19954(3):343-3469TeradaS,MatsuuraK,EnosawaS,etal.Inducingproliferationofhumanamnioticepithelial(HAE)cellsforcelltherapy.CellTransplant20009(5):701-70410BailoM,SonciniM,VertuaE,etal.Engraftmentpotentialofhumanamnionandchorioncellsderivedfromtermplacenta.Transplantation200478(10):1439-144811LiH,NiederkornJY,NeelamSetal.Immunosuppressive.factorssecretedbyhumanamnioticepithelialcells.OphthalmolVisSci200546(3):900-90712KamiyaK,WangM,UchidaS,etal.Topicalapplicationofculturesupernatantfromhumanamnioticepithelialcellssuppressesinflammatoryreactionsincornea.ExpEyeRes200580:671-67913RambhatlaL,ChiuCP,KunduP,etal.Generationofhepatocyte-likecellsfromhumanembryonicstemcells.CellTransplant200312(1):1-1114DanetGH,LuongoJL,ButlerG,etal.C1qRpdefinesanewhumanstemcellpopulationwithhematopoieticandhepaticpotential.ProcNatlAcadSciUSA200299(16):10441-1044515KakinumaS,TanakaY,ChinzeiR,etal.Humanumbilicalcordbloodasasourceoftransplantablehepaticprogenitorprogenitorcells.stemcell200321:217-22716EnosawaS,SakuragawaN,SuzukiS.Possibleuseofamnioticcellsforregenerativemedicine.Nip