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编号引物名称序列(5-3)S01M13RCAGGAAACAGCTATGACCS02M13FTGTAAAACGACGGCCAGTS03M13F(-47)CGCCAGGGTTTTCCCAGTCACGACS04M13R(-48)AGCGGATAACAATTTCACACAGGAS05M13(-96)CCCTCATAGTTAGCGTAACGS06SP6ATTTAGGTGACACTATAGS07T7(18mer)TAATACGACTCACTATAGS08T7terTGCTAGTTATTGCTCAGCGGS09S.tagGAACGCCAGCACATGGACS10T3ATTAACCCTCACTAAAGGGAS11T7(20mer)TAATACGACTCACTATAGGGS12BGHTAGAAGGCACAGTCGAGGS13CMV-ProforCGCAAATGGGCGGTAGGCGTGS14EBVrevGTGGTTTGTCCAAACTCATCS15pGEX-5GGGCTGGCAAGCCACGTTTGGTGS16pGEX-3CCGGGAGCTGCATGTGTCAGAGGS17GLp1TGTATCTTATGGTACTGTAACTGS18GLp2CTTTATGTTTTTGGCGTCTTCCAS19RVP3CTAGCAAAATAGGCTGTCCCS20RVP4GACGATAGTCATGCCCCGCGS21pCMV-FTCTAAAAGCTGCGGAATTGTS22pCMV-RTCCAAACTCATCAATGTATCS23pIRES2-EGFP.P5GTAGGCGTGTACGGTGGGAGS24pIRES2-EGFP.P3AACGCACACCGGCCTTATTCS25GAL4-AD5AATACCACTACAATGGATGATGS263ADAGATGGTGCACGATGCACAGS27S1CAACGTGAAAAAATTATTATTCGCS28S6GTAAATGAATTTTCTGTAGTAGGS295-AOXGACTGGTTCCAATTGACAAGCS303-AOXGCAAATGGCATTCTGACATCCS31α-FactorTACTATTGCCAGCATTGCTGCS32pEGFP-N-5TGGGAGGTCTATATAAGCAGAGS33pEGFP-N-3CGTCGCCGTCCAGCTCGACCAGS34pEGFP-C-5CATGGTCCTGCTGGAGTTCGTGS35pEGFP-C-3TATGGCTGATTATGATCAGTS36PBV220FAAGAAGGGCAGCATTCAAAGS37PBV220RCTGCGTTCTGATTTAATCTGS38pQE30-FTGAGCGGATAACAATTTCACS39pQE30-RGTTCTGAGGTCATTACTGGS40PDSRED-N-35'-GTACTGGAACTGGGGGGACAG-3'S41pDsRED-C1-5AGCTGGACATCACCTCCCACAACGS42pDsRed-C-3ATTCATTTTATGTTTCAGGTTCAS43pFastBac-5TATTCCGGATTATTCATACCS44pFastBac-3TTCAGGTTCAGGGGGAGGTGS45BAC1AACCATCTCGCAAATAAATAS46BAC2ACGCACAGAATCTAGCGCTTS47GAL4-BD5TCATCGGAAGAGAGTAGS483BDTAAGAGTCACTTTAAAATTTGTATACS49pShuttle-CMV-FGGTCTATATAAGCAGAGCTGS50pShuttle-CMV-RGTGGTATGGCTGATTATGATCAGS51MalEPrimerGGTCGTCAGACTGTCGATGAAGCCS52pEGU6a-1GCTTTCTTCCCTTCCTTTCTS53N-CMV-30ATAACCCCGCCCCGTTGS54C-CMV-24TTAGGACAAGGCTGGTGGS55pTRC99C-FTTGCGCCGACATCATAACS56pTRC99C-RCTGCGTTCTGATTTAATCTGS57pCEP-FAGAGCTCGTTTAGTGAACCGS58pB42ADFCCAGCCTCTTGCTGAGTGGAGATGS59pB42ADRAAGCCGACAACCTTGATTGGAGS60U6primerATGGACTATCATATGCTTACCGTAS612.0revAGGCGATTAAGTTGGGTAS623.0revGAGTTAGCTCACTCATTAGGCS635TriplEx2CTCCGAGATCTGGACGAGCS64pEF-FTCAAGCCTCAGACAGTGGTTCS64EF-1aForwardTCAAGCCTCAGACAGTGGTTCS65pCMV5RATTATAGAGGACACCTAGTCS66pCMV5FTTCCAAAATGTCGTAATAACS67pFlag-CMV-FGGTAGGCGTGTACGGTGGS68pFlag-CMV-RGCACTGGAGTGGCAACTTS69pLEXA-FCGTCAGCAGAGCTTCACCATS70pLEXA-RTAAAACCTAAGAGTCACTTTS71pLNCX-FAGCTCGTTTAGTGAACCGTCAGATCGS72pLNCX-RACCTACAGGTGGGGTCTTTCATTCCCS73pLXSN-FCTTGAACCTCCTCGTTCGACS74pLXSN-RGTTGCTGACTAATTGAGATGS75pIRES-FCTTACTGACATCCACTTTGCS76pIRES-RCACTGCATTCTAGTTGTGGTS77PinPointprimerCGTGACGCGGTGCAGGGCGS78P5TGCGTACTGCGGTGATCAACS79P3CTGCAAGGCGATTAAGTTGGS80pTarget.FCCAGGATTTTCCCAGTCACS81pTarget.RGGCTTTACACTTTATGCTTCS82pYes2.RTCGGTTAGAGCGGATGTGS83pAS2-1.RAAGAGTTACTCAAGAACAAGAAS84P1CCAGGCTTTACACTTTATGCS85P2GCGATTAAGTTGGGTAACGCS86pBAD-5CCATAGCATTTTTATCCATAAGS87pBAD-3ATCTGTATCAGGCTGAAAATCS88SV40pA-RGAAATTTGTGATGCTATTGCS89pAd-CMVGCTAGAGATCTGGTACCGTCS90pAdTrack-CMV-FGAAGTGAAATCTGAATAATTTTGTS91pAdTrack-CMV-RCAAAACTACATAAGACCCCCACS92pMX-S1811GACGGCATCGCAGCTTGGATACACS93pDC316FACGTGGGTATAAGAGGCGS94pDC316RCGATGCTAGACGATCCAGS95PBT-FTCCGTTGTGGGGAAAGTTATCPBT-RGGGTAGCCAGCAGCATCCS96PRB391上游AAAGCTTCCGGAGGTCCCGAGGGCGGCAGCS97PVL1393forwardAAATGATAACCATCTCGCS98PBABE-5ctttatccagccctcacS99PBABE-3accctaactgacacacattccPBABE-RCCTCACTACTTCTGGAATAS100PTRG-F(18bp)TGGCTGAACAACTGGAAGS101PMSCV-5CCCTTGAACCTCCTCGTTCGACCS102PMSCV-3GAGACGTGCTACTTCCATTTGTCS103ColidownTTCACTTCTGAGTTCGGCATGS104PTRG-R(GMM-R)ATTCGTCGCCCGCCATAAS105pTrcHis_fwdGAGGTATATATTAATGTATCGS106pTrcHis_revGATTTAATCTGTATCAGGS107pGCsi-U6FAGCTACAACAAGGCAAGGS108pGCsi-U6RTAGAAGGCACAGTCGAGGS109pMT2-FTTGCCTTTCTCTCCACAGGTS110PVL1393reverseGGTCCAAGTTTCCCTGTS111PGL4-RACTCGGCGTAGGTAATGTCCS11235S(PBI-121)GACGCACAATCCCACTATCCS113U6ForwardGGGCAGGAAGAGGGCCTATS114KSTCGAGGTCGACGGTATCS115SKCGCTCTAGAACTAGTGGATCS116Duetup1(pETupstream)ACGATGCGTCCGGCGTAGAS117Duetdown1GATTATGCGGCCGTGTACAAS118Duetup2TTGTACACGGCCGCATAATCS119pCAG-FGCAACGTGCTGGTTATTGTGS120pJET1.2ReverseAAGAACATCGATTTTCCATGGCAGS121AC5ForwardACACAAAGCCGCTCCATCAGS122MTForwardCATCTCAGTGCAACTAAAS123U6-pLL3.7cagtgcaggggaaagaatagtagacS124LucNrevCCTTATGCAGTTGCTCTCCS125pJET1.2ForwardCGACTCACTATAGGGAGAGCGGCS126V5CtermRGAGGAGAGGGTTAGGGATAGS127PQC-5primeracgccatccacgctgttttgacctS128PQC-3primerAAGCGGCTTCGGCCAGTAACGTTAS129pBMN5（PMXs1811）GACGGCATCGCAGCTTGGATACACS130WPRE-rACAAAGGCATTAAAGCAGCGS131UBforwardprimerTCAGTGTTAGACTAGTAAATTGS132PUAST-FTCAACTGCAACTACTGAPUAST-RGTCACACCACAGAAGTAAGGS133EMGFPForwardPrimerTACAAGGGCTCGAGCCCATCAAS134NOS5-3TGGCAAGACCGGCAACAGGATTCAAS135pCold-FPrimerCGCCATATCGCCGAAAGGCS136pCold-RPrimerGGCAGGGATCTTAGATTCTGS137hGH-PA-RCCAGCTTGGTTCCCAATAGAS138TK-pA-RTTGTCTCCTTCCGTGTTTCAS139PL451-SGTTTTCCCAGTCACGACGTTS140PL451-ASGCTTGGCTGGACGTAAACTCS141LKO.15 GACTATCATATGCTTACCGTS142ITS1TCCGTAGGTGAACCTGCGGS143ITS4TCCTCCGCTTATTGATATGCS144promoter-FAACGCAATTCCAGTTACTCS145pll3.7FccgctctagagatccgacgS146PLL3.7primerttaataactaatgcatggcggS147pSUPER_retro_puro-FGGAAGCCTTGGCTTTTGS148pSUPER_retro_puro-RGATGACGTCAGCGTTCGS149PHW2000RCCCGGGGGAGGTATATCTS150T7(19mer)TAATACGACTCACTATAGGS151pTRE-5CGCCTGGAGACGCCATCCS152pTRE2-3CATTCTAAACAACACCCTGAS153pTRE-3CCACACCTCCCC