马齿苋提取液

PortulacaExtract马齿苋提取液Naturalproduct天然产品PortulacaExtract目录CONTENTSⅠ.Desc1.PortulacaExtract马齿苋提取液1)Introduction简介2)Maineffect主要作用3)Application应用Ⅱ.Evaluation1.CellCytotoxity测试马齿苋提取液对细胞的毒性2.AllergyEstimation过敏性评估3.Anti-Inflammatory测试马齿苋提取液抗炎症、抗红肿的功效4.Anti-Irritation测试马齿苋提取液抗刺激的功效5.SafetyTest安全性评估PortulacaExtractPortulacaExtract马齿苋提取液INCIName:PortulacaOleraceaExtractINCI名称：马齿苋提取液Chemicalclasses:BiologicalProduct化学分类：生物学产品Technical/OtherName技术/其它名称ExtractofPortulacaOleracea马齿苋提取物PortulacaOleracea(EU)马齿苋（EU）ExtractMatter:WholePlant提取部位：整个植物Description描述PortulacaExtractisdevelopedasabasicingredientforcosmetics,toiletriesandpharmaceuticalswithanti-inflammatoryandanti-irritationeffect马齿苋提取液作为一种基础成分可用于化妆品，盥洗品和抗发炎，抗刺激的药物。PortulacaExtractMainEffect主要作用Anti-InflammationAnti-Irritation抗发炎抗刺激Dandruff头皮屑Dandruff头皮屑AtopicDermatitis过敏性皮炎AtopicDermatitis过敏性皮炎Erysipelas丹毒Erysipelas丹毒Hairloss脱发Hairloss脱发Acne粉刺Acne粉刺Insectbite昆虫叮咬Insectbite昆虫叮咬PortulacaExtract马齿苋提取液PortulacaExtractApplication应用•Cosmetics•化妆品1.SkinCare(Skin，Lotion,Essence,cleanseretc..)皮肤护理（皮肤，乳液，精华素，清洁剂等）2.BabyCare(Skin,soap,bathetc..)婴儿护理（皮肤，肥皂，洗澡等）3.FootCare(Footcreametc..)足部护理（足部膏霜）•Toiletries•盥洗用品1.HairCare(Shampoo,Rinse,Hairessenceetc…)头发护理（香波，嗽口水，护发素等）2.BodyCare(Soap,Bath&ShowerGel,Bodycreametc..身体护理（香皂，盆浴&沐浴露，体用膏霜等）•Pharmaceuticals药物1.Acne粉刺2.AtopicDermatitis过敏性皮炎3.Footcream足部膏霜RecommendedDosage:3.0~5.0%推荐用量：3.0~5.0%BodyFootAcneHair有效成分202%糖，氨基酸其他成分马齿苋黄酮，马齿苋生物碱主要有效成分1PortulacaExtract产品名称:马齿苋提取液分析测试规格INCI名称来源CASNo.EINECSNo.外观气味pH(1→10,20℃)比重(d20/20)重金属含量砷含量杀虫剂残留微生物含量马齿苋提取液马齿苋90083-07-1290-201-1黄色液体特征气味5.0±1.01.000~1.040.≤10ppmppm≤.2ppm100cfu/㎖Specification规格≤≤≤外观：黄色液体在正常的储存条件下产品稳定PortulacaExtractMaterialSafetyDataSheet物化数据1.2.气味：特征气味3.密度：1.020±0.040(20℃)4.自燃温度：400℃5.爆炸极限：低于2.4vol%高于13.5vol%(仅对于纯的1,3-丁烯二醇)6.水溶性：可溶7.有机溶剂中的溶解性：溶解于多种有机溶剂8.纯产品的pH值：5.0±1.0（1→10，20℃）稳定性PortulacaExtract1.CellCytotoxity测试马齿苋提取液对细胞的毒性2.AllergyEstimation过敏性评估3.Anti-Inflammatory测试马齿苋提取液抗炎症、抗红肿的功效4.Anti-Irritation测试马齿苋提取液抗刺激的功效5.SafetyTest安全性测试Evaluation功效性评估PortulacaExtract01020304050607080901E-061E-051E-041E-030.010.1110(%,W/V)MTT–AssayP.Extract(IC50:5%)S.L.S(IC50:0.0025%)CellCytotoxicity对细胞的毒性Degreeofviability(%)ㄹFig1.CellcytotoxicityofPortulacaExtractagainstV79-4derivedfromlungtissuefibroblastofChinesehamster.图1.马齿苋提取液对老鼠肺部组织纤维原细胞V79-4的细胞毒性PortulacaExtractAllergyEstimation过敏性评估050010001500200025003000350040004500500060%EtOHP.E(50%)P.E(100%)CPMFig2.Radioactivityof3H-methylthymidineincorporatedintolymphocytetreatedwithP.E.(PortulacaExtract)图2.用马齿苋处理过的甲基胸(腺嘧啶脱氧核)苷合成的淋巴细胞的放射性计数LocalLymphNodeAssay局部淋巴细胞化验226145483187CPM(CountingPerMinute)（以每分钟计数）;Quantityof［H3］-methylthymidineincorporatedIntolymphocyte.甲基胸(腺嘧啶脱氧核)苷合成的淋巴细胞的数量S.I(StimulationIndex)（刺激指数）;AverageCPMoftestmaterial/AverageCPMofcontrol测试材料组的CPM平均数/空白组的CPM平均数PortulacaExtractAnti-inflammatory抗发炎性(1)051015202530354045500.50%1.00%3.00%1.00%MouseEarEdemaAssay老鼠耳部水肿化验Inhibitionrateofedema(%)Fig3-1.InhibitionrateofedemabylocalapplicationofPortulacaExtract图3-1.局部使用马齿苋提取液后对水肿的抑制率15.5%20.2%26.6%45.6%PortulacaEx.马齿苋提取液PortulacaEx.马齿苋提取液PortulacaEx.马齿苋提取液Indomethacin茚甲新InhibitionRate(%)=(A-B)/Ax100抑制率A:AverageswellinginControlgroup空白组平均肿胀B:AverageswellinginTestgroup测试组平均肿胀PortulacaExtractAnti-inflammatory抗发炎性(2)Localapplicationofskinbase051015202530Skinbase-Portulaca1.0%Portulaca3.0%Portulaca5.0%Inhibitionrate(%)Fig3-2.InhibitionrateofedemabylocalapplicationofskinbasescontainingPortulacaExtract图3-2.皮肤局部用马齿苋提取液处理后对皮肤水肿的抑制率0%19.5%24.1%28.0%PortulacaExtractAnti-irritation抗刺激性(1)实验材料*5%的马齿苋提取液(BiolandLtd.,/LotNo.:NMP-L-122663)1.志愿者45个志愿者年龄在25~55岁之间男性16女性252.使用剂量0.01g3.方法•*将0.01g马齿苋提取液滴入chamber中，然后将此finnchamber粘贴到志愿者的上臂，贴足24小时后取下。•*在取下马齿苋提取液贴片24小时后,评估皮肤的状况。测试方法(人体斑贴测试)/由日本头发科学协会实验室测试结论48h24h––纯净水(空白组)5%马齿苋提取液(测试组)材料––取下贴片后“–“:根据国际接触性皮炎研究组的标准无过敏反应PortulacaExtractAnti-irritation抗刺激性(2)Fig4.AverageresponsedegreebylocalapplicationofPortulacaExtract图4.皮肤局部用马齿苋提取液处理后的平均敏感程度AverageResponseDegreeHumanpatchTest0.440.180.120.1200.050.10.150.20.250.30.350.40.45SLS(0.08%)P.E1%(SLS0.08%)P.E.3%(SLS0.08%)D/WPortulacaExtractSafetyTest*TestedbyTanabeR&DServiceCo.,Ltd/JAPAN*™TestMaterial测试材料Name:PortulacaExtract名称：马齿苋提取液LotNo.:NMP-L-010893Description:Yellow,fluid外观：黄色液体Specificgravity:1.000~1.040比重：1.000~1.040pH:5.0±1.0Stability:Stableatroomtemperature稳定性：在常温下稳定1.Acuteoraltoxicitystudyinrats对老鼠的口服毒性测试2.Primarycutaneousirritationtestinrats对老鼠皮肤的一次性刺激性测试3.Continuouscutaneousirritationtestinrabbits对兔子皮肤的连续性刺激性测试4.Eyeirritationtestinrabbits对兔子眼睛的刺激性测试5.Guineapigmaximizationtest对豚鼠的最大耐受性测试6.Reversemutationtestusingbacteria对细菌的细胞突变测试7.Chromosomeaberrationtestinculturedcells.对培养的细胞的染色体畸变测试PortulacaExtractSafetyTest*TestedbyTanabeR&DServiceCo.,Ltd/JAPAN*SexDose(mg/kg)GeneralConditionAfteradministrationMale(Control)0a)WaterforinjectionNormalNormalNormalNormalNochangeMale2000NochangeFemale(Control)0a)WaterforinjectionNochange2000AutopsyfindingFemaleNochange1.AcuteOralToxicityStudyinRats•Animalsused:Sprague-DawleyRats.•Aged:6weeks•Weight-Male:164.7g~177.7g-Female:135.0g~