SnapGene-User-Guide

SnapGeneUserGuideSnapGeneUserGuideLicenses1DownloadandRegisterSnapGene61.1GenerateanEmailwithaDownloadLink91.2UnregistertheComputerYouAreUsing111.3UnregisteraComputerRemotely131.4RetrieveaLicensefromaComputer151.5SetUpaLicenseServeronaMac171.6SetUpaLicenseServeronWindows241.7SetUpaLicenseServeronLinux311.8Enzymes2ShoworHideEnzymes362.1ChooseanEnzymeSet382.2ChooseEnzymesManually392.3ViewaRestrictionSiteOverview452.4SortEnzymes472.5ViewEnzymeInformation502.6ViewNoncutters532.7SaveorExportEnzymeSet552.8ManageEnzymeSets572.9SetEnzymePreferences622.10Features3CreateaSimpleFeature653.1CreateaTranslatedFeature683.2CreateaFeatureSegment723.3AnnotateanIntron743.4AddaCleavageArrow773.5EditMultipleFeatures793.6DeleteFeatures813.7ConfirmanIn-FrameGeneFusion823.8SettheGeneticCodeforaFeature843.9DesignateaNon-ATGStartCodon863.10HideorShowFeatures883.11SorttheFeatureList923.12ImportFeatureData943.13ExportFeatureData993.14SplicetoRemoveIntrons1013.15DetectCommonFeatures1033.16DetectCustomFeatures1073.17EditCommonFeatures1113.18ShareCustomFeatureList1153.19Primers4CreateaPrimer1214.1EditMultiplePrimers1244.2AdjustPrimerHybridizationParameters1264.3HideorShowPrimers1284.4SortthePrimersList1314.5ImportPrimers1334.6ExportPrimers1404.7Translations5ShoworHideTranslationsandORFs1435.1ChoosetheTranslationFrames1455.2ChoosetheTranslationFormat1475.3ChoosetheTranslationGeneticCode1495.4DisplayOpenReadingFrames1535.5ConvertanORFtoaFeature1555.6Colors6ShoworHideColors1586.1CustomizetheSequenceColors1616.2ChooseaColorMode1656.3ShowColorsinHistoryView1686.4Searching7SearchforaDNASequence1717.1SearchforaProteinSequence1757.2SearchforanEnzyme,Feature,orPrimer1807.3Zooming8ShoworHidetheZoomControls1858.1ZoomIn1878.2PanAcrossaSequence1908.3SnapGeneUserGuide-5LicensesSnapGeneUserGuide-6DownloadandRegisterSnapGeneAfterpurchasingSnapGene,howcanIdownloadandregisterthesoftware?ObtaintheDownloadLinkfromEmailAfterpurchase,theaccountmanagerwillreceiveanemail(shownabove)withadownloadlink.ThisemailcanbeforwardedtoothersinthegroupwhowillbeusingSnapGene.Atanytime,theaccountmanagercangenerateanewemailtobeforwarded(seelesson:GenerateanEmailwithaDownloadLink).DownloadtheAppropriateVersionofSnapGeneThelinkintheemailwilltakeyoutoawebpagewithtwodownloadbuttons.ClickonthebuttonforeithertheMacortheWindowsversionofSnapGene.SnapGeneUserGuide-7InstallandRunSnapGeneInstallthesoftware.Whenyourunitforthefirsttime,theSnapGeneRegistrationdialogwillappear.ObtaintheGroupNameandRegistrationCodeOnthesamewebpageasthedownloadbuttons,youwillfindthegroupnameandregistrationcode.SnapGeneUserGuide-8RegisterSnapGeneEntertheGroupNameandRegistrationCode.Youcancopytheseitemsfromthewebpage,andpastethemintheSnapGeneRegistrationdialog.ThenclickRegister.BeginUsingSnapGeneAfterafewmoments,SnapGenewillconfirmtheregistration.CickOK.Youmustbeconnectedtotheinternettoregister.Fromthenon,SnapGenewillrunwithoutrequiringaninternetconnection.SnapGeneUserGuide-9GenerateanEmailwithaDownloadLinkAstheaccountmanager,howcanIsendanemailwithadownloadlinktotheauthorizedSnapGeneusersinmygroup?GototheLoginPageForthisprocedure,youmustbetheaccountmanagerfortheSnapGenelicenses.Gotosnapgene.com,andclickontheManageMyAccountlinkintheupperrightcorner.LoginEntertheemailaddressandpasswordfortheaccountmanager.Theoriginalpasswordwassentinanemailimmediatelyafterpurchase.Ifyouhavelostthepassword,clickthetriangletoshowcontrolsforobtaininganewpasswordbyemail.EmailYourselfaDownloadLinkOntheaccountpage,clickSendEmailtosendyourselfanemailwithadownloadlink.ForwardthisemailtotheauthorizedSnapGeneusersinyourgroup.SnapGeneUserGuide-10LogoutWhenyouarefinished,clickontheLogoutlinkintheupperrightcorner.SnapGeneUserGuide-11UnregistertheComputerYouAreUsingHowcanIunregisterSnapGeneonthecomputerIamcurrentlyusing,freeingthelicenseforuseonanothercomputer?OpenandExpandtheRegistrationDialogToopentheRegistrationDialog,clickSnapGene→Registration(Mac)orHelp→Registration(Windows).ExpandtheRegistrationdialogbyclickingtheHelp!triangle.ChoosetheSecondOptionChooseIwouldliketounregisterthiscomputer.ClickUnregisterThisComputer.SnapGeneUserGuide-12CompletetheUnregistrationSnapGenewillnolongerrunonthesamecomputerunlessyouregisteragain.Thelicenseisavailableforuseonanothercomputer.SnapGeneUserGuide-13UnregisteraComputerRemotelyAstheaccountmanager,howcanIunregisteraSnapGenelicenseonacomputerthatisnotphysicallyaccessible?GototheLoginPageForthisprocedure,youmustbetheaccountmanagerfortheSnapGenelicenses.Gotosnapgene.com,andclickontheManageMyAccountlinkintheupperrightcorner.LoginEntertheemailaddressandpasswordfortheaccountmanager.Theoriginalpasswordwassentinanemailimmediatelyafterpurchase.Ifyouhavelostthepassword,clickthetriangletoshowcontrolsforobtaininganewpasswordbyemail.DisplaytheActiveLicensesOntheaccountpage,clicktheActiveLicensestriangletoshowtheSnapGenelicensesinusebyyourgroup.SnapGeneUserGuide-14DeactivatetheLicenseRemotelyTodeactivatealicense,presstheDeactivatebuttonnexttothenameoftherelevantcomputer.ThecomputerwillnolongerbelistedintheActiveLicens