HACH-哈希-TP快速测定方法

Phosphorus,TotalPage1of8Phosphorus,Total,8190Howtouseinstrument-specificinformationTheInstrument-specificinformationtabledisplaysrequirementsthatmayvarybetweeninstruments.Tousethistable,selectaninstrumentthenreadacrosstofindthecorrespondinginformationrequiredtoperformthistest.Phosphorus,TotalDOC316.53.01121USEPA1PhosVer®3withAcidPersulfateDigestionMethodMethod81901USEPAAcceptedforreportingwastewateranalyses(StandardMethods4500P-E).0.06to3.50mg/LPO43–or0.02to1.10mg/LPTest‘NTube™VialsScopeandApplication:Forwater,wastewaterandseawaterTestpreparationTable1Instrument-specificinformationInstrumentLightshieldAdapterDR5000——DR2800LZV646—DR2700LZV646—DR/2500——DR/2400—5945700Beforestartingthetest:DR2800andDR2700only:InstallthelightshieldinCellCompartment#2beforeperformingthistest.Formoreaccurateresults,determineareagentblankvalueforeachnewlotofreagent.Followtheprocedureusingdeionizedwaterinplaceofthesample.Subtractthereagentblankvaluefromthefinalresultsorperformareagentblankadjust.Thetestrangefortotalphosphateislimitedto0.06to3.5mg/LPO43–.Valuesgreaterthan3.5mg/Lmaybeusedtoestimatedilutionratios,butshouldNOTbeusedforreportingpurposes.Ifthevalueisgreaterthan3.5mg/L,dilutethesampleandrepeatthedigestionandthecolorimetrictest.Finalsampleswillcontainmolybdenum.Inaddition,finalsampleswillhaveapHlessthan2andareconsideredcorrosive(D002)bytheFederalRCRA.RefertothecurrentMSDSforsafehandlinganddisposalinstructions.Phosphorus,TotalPage2of8Phosphorus,TotalCollectthefollowingitems:DescriptionQuantityTotalPhosphorusTest‘NTube™ReagentSet1DeionizedwatervariesDRB200Reactor1Funnel,micro1LightShieldorAdapter(seeInstrument-specificinformation)1Pipet,TenSette®,1to10mL,plustips1TestTubeRack1SeeConsumablesandreplacementitemsforreorderinformation.PhosVer3,acidpersulfatedigestion1.TurnontheDRB200Reactor.Preheatto150°C.2.Selectthetest.Insertanadapterifrequired(seeInstrument-specificinformation).3.UseaTenSette®Pipettoadd5.0mLofsampletoaTotalPhosphorusTestVial.4.UseafunneltoaddthecontentsofonePotassiumPersulfatePowderPillowforPhosphonatetothevial.5.Captightlyandshaketodissolve.6.InsertthevialintotheDRB200.Closetheprotectivecover.7.Settheinstrumenttimerto30minutesandstart.A30-minuteheatingperiodwillbegin.8.Whenthetimerexpires,carefullyremovethehotvialfromthereactor.Insertitinatesttuberackandcooltoroomtemperature.536PTotal/AHPVTNTStoredProgramsStartPhosphorus,TotalPhosphorus,TotalPage3of8Interferences9.UseaTenSettePipettoadd2mLof1.54NSodiumHydroxideStandardSolutiontothevial.Capandmix.10.Wipetheoutsideofthevialwithadampclothfollowedbyadryone.11.Insertthevialintothe16mmcellholder.12.ZEROtheinstrument.Thedisplaywillshow:0.00mg/LPO43–13.UseafunneltoaddthecontentsofonePhosVer3PowderPillowtothevial.14.Immediatelycaptightlyandshaketomixfor20–30seconds.Thepowderwillnotdissolvecompletely.15.Starttheinstrumenttimer.Atwo-minutereactionperiodwillbegin.Readthesamplewithin2–8minutesafterthetimerexpires.16.Afterthetimerexpires,wipetheoutsideofthevialwithawettowel,thenadryone.Insertthepreparedsamplevialintothe16mmcellholder.READtheresultsinmg/LPO43–.Table2InterferingsubstancesInterferingsubstanceInterferencelevelAluminumGreaterthan200mg/LArsenateInterferesatanylevelChromiumGreaterthan100mg/LCopperGreaterthan10mg/LIronGreaterthan100mg/LNickelGreaterthan300mg/LpH,excessbufferingHighlybufferedsamplesorextremesamplepHmayexceedthebufferingcapacityofthereagentsandrequiresamplepretreatment.SilicaGreaterthan50mg/LPhosVer3,acidpersulfatedigestion(continued)ZeroPhosphorus,TotalPage4of8Phosphorus,TotalSamplecollection,preservationandstorage•Collectsamplesinplasticorglassbottlesthathavebeenacidwashedwith1:1HydrochloricAcidSolution*andrinsedwithdeionizedwater.•Donotusecommercialdetergentscontainingphosphateforcleaningglasswareusedinthistest.•Analyzethesamplesimmediatelyforthemostreliableresults.•Ifpromptanalysisisnotpossible,samplesmaybepreservedupto28daysbyadjustingthepHto2orlesswithconcentratedSulfuricAcid*(about2mLperliter)andstoringat4°C.•Warmstoredsamplestoroomtemperatureandneutralizewith5.0NSodiumHydroxide*beforeanalysis.•Correcttestresultsforvolumeadditions.AccuracycheckStandardadditionsmethod(samplespike)Requiredforaccuracycheck:•Phosphate10-mLAmpuleStandard,50-mg/LasPO43–•Ampulebreaker•TenSettePipet•Mixingcylinders,(3)1.Cleanglasswarewith1:1HydrochloricAcidStandardSolution.Rinseagainwithdeionizedwater.Donotusephosphatedetergentstocleanglassware.2.Afterreadingtestresults,leavethesamplecell(unspikedsample)intheinstrument.3.Selectstandardadditionsfromtheinstrumentmenu:4.Acceptthedefaultvaluesforstandardconcentration,samplevolumeandspikevolumes.Afterthevaluesareaccepted,theunspikedsamplereadingwillappearinthetoprow.Seetheusermanualformoreinformation.SilicateGreaterthan10mg/LSulfideGreaterthan90mg/LTurbidityorcolorMaycauseinconsistentresultsbecausetheacidinthepowderpillowmaydissolvesomeofthesuspendedparticlesandbecauseofvariabledesorptionoforthophosphatefromtheparticles.ZincGreaterthan80mg/L*SeeOptionalreagentsandapparatus.InstrumentNavigateto:DR5000OPTIONSMORESTANDARDA