PCR及western耗材订购

实验试剂数量货号品牌单价RNA提取提取RNA试剂盒（或者trizol，invotrigen）Trizol200ml装15596018Invitrogen2350Trizol100ml装155960261350RNA反转反转录cDNA试剂盒盒k1622Invitrogen13845*TBE1瓶荧光定量PCRpowersybrgreen瓶-5ml4367659ABI1680实验消耗无RNA酶1.5ml离心管1盒mct-150-cAxygen65无RNA酶1000ul枪头1包t-1000-bAxygen80无RNA酶200ul枪头2包T-200-YAxygen75无RNA酶10ul枪头2包t-300Axygen750.2mlPCR管1包PCR-02-CAxygen20096孔板封口膜1盒4360954ABI68596孔板（pcr用）1盒4346906ABI855操作步骤试剂数量货号品牌单价蛋白抽提、蛋白浓度测定、蛋白变性RIPA裂解液100mlC1053普利莱135蛋白酶抑制剂混合物1mlCW2200康维世纪142蛋白质定量试剂盒(BCA法)500assayp1511普利莱2975XSDS-PAGESample/LoadingBuffer(inhibitingdephosphorylation)5mlB1012普利莱75配分离胶和浓缩胶4×浓缩胶缓冲液200mlB1003普利莱1084×分离胶缓冲液500mlB1004普利莱10830%丙烯酰胺-双丙烯酰胺溶液200mlB1000普利莱162TEMED，四甲基乙二胺，NNNN-tetramethylethyl5mlA1006普利莱45过硫酸胺Ammoniumpersulfate100GA1004普利莱180电泳、电转、洗涤、封闭10×SDS-PAGE电泳缓冲液500mlB1005普利莱10810×蛋白电转移缓冲液(10×Transferbuffer)500mlB1006普利莱10810×封闭-洗涤缓冲液(TBST)500mlB1009普利莱108封闭专用脱脂奶粉250gP1622-2普利莱216蛋白转移膜PVDF膜0.45ummilipoerIPVH00010Millipore1650转膜专用滤纸（bia-rad）1703932Biorad435普通蛋白marker普通蛋白marker250ul*226616ThermoScientific688化学发光试剂ECL化学发光检测试剂盒100ml34077Pierce926D72显影粉包E1201普利莱12D72定影粉包E1202普利莱12膜再生液膜再生液瓶SW3020索莱宝90