常规HE染色易出现的问题_原因及解决方法

©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(,100700)[]HE;;[]R36112[]B[]1007-8096(2008)06-0500-03HEHE,,11.1,,;,,,,;,,,,,:5%20%10s;,1.2,,,;;,;;,,,95%,,,,,,1.3;,,;,,,,pH715718,;HE,1%,,1%,,,,,1%,,,,,1%,;,;;,,22.1,,,,2.2,,,,,2.32.4,,,;,;,;,,3()3.1,,,,,,,3.2,,,;,,,,,,,,,,005JDiagPathol,December2008,Vol.15,No.6©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.:,,,,,,,,,:4%,(),,,,510min,95%80%,,3%5min3.3,,,3.4,3.5,,,,;,,3.6,3.7,;,,3.8,;(),,44.1,,4.2,,():;,1%,;,4.3,5,,,:,,;,,,,:12h;,12h;,1%,,;,5%30min,,;,,,3%,,;,6,,,:,,;6.1(),pH;,,,6.26.3HE,,,pH7158,,,,,,100ml12,,,,6.46.5,,,,105200812156©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.:,,,;;,,;,,;:,-(13),;,8HE,,,,,:;9;,;;,,HE(),,,,,,,,,:2008-07-07,,,(175,363000)[];;[]R36112[]B[]1007-8096(2008)06-0502-02,,1111175,p63c2erbB22ERPRMaxVision,11270,4%4min;1mm,2min;,46min,;(70)5min,,;,,,35m,,DAB,(512)205JDiagPathol,December2008,Vol.15,No.6