分光光度法测定滇池藻类生长的研究_段刚

2006,Vol.23No.4Chemistry&Bioengineering55:国家重点基础研究发展计划资助项目(2002CB412310):2005-12-09:段刚(1958-),男,吉林长春人,高级工程师,主要从事污染控制及环境影响评价研究电话:[phone],E-mail:[e-mail]1,1,2,2,2,3(1.云南省环境科学研究院,云南昆明650034;2.云南民族大学化学与生物工程学院,云南昆明650031;3.云南大学化学与材料科学学院,云南昆明650031):在滇池水域中取藻样,过滤接种在MDM培养液中,置于光照温室中培养,提取出叶绿素,然后测定在不同波长下的吸光度值,用叶绿素法计算公式计算出叶绿素a含量,可间接地测定滇池藻类的生长情况实验研究结果表明,滇池藻类的叶绿素吸收光谱有两个最强的吸收区:一个是在波长为400~460nm的蓝紫光部分,另一个是在波长为640~660nm的红光部分采用分光光度法在645nm波长处测定Zn2+对滇池藻类生长的影响,效果较好:滇池;叶绿素a;藻类;波长;吸光度值:Q9492:A:1672-5425(2006)04-0055-03[1~4],[5~9],,,:,,,,[10,11],,,,,,,[12]1997419985,5[13],2038,90%[13],,,,,a,645nmZn2+,111:,,PTFE;;(10000mgL-1),MDM11MDMTab.1ComponentofMDMculturalbaseliquorKNO3MgSO47H2OK2HPO4NaClCaCl22H2O/gL-11002502501001TDL-40B,;WFJ-7200,:/200645612121MDM100mL,1L,10molL-1NaOHpH,()pH(pH=63),50mL,10mL,PTFE,400050lx12h12h(251)122aa-,4000rmin-16min,,10mL24h1cm,,OD(),a(Chla)[14]Chla=(1164OD669-216OD645+01OD630)VaVc:VaVc,mL;Chla,gL-1123,,,Zn2+,121,Zn2+,,OD221,,,,300nm2600nm,390~770nm,400~700nm[15],,320nm340nm360nm380nm400nm420nm440nm460nm480nm500nm520nm540nm560nm580nm600nm620nm640nm660nm700nm720nm740nm780nm,:400~460nm;640~660nm,1,645nm,Zn2+1Fig.1Changecurveofabsorbancewithwavelength22Zn2+aZn2+,a,Zn2+a,aZn2+22aFig.2InfluenceofconcentrationofZn2+oncontentofchlorophylla2,001mgL-1,,a,,;001mgL-1,a,,23Zn2+Zn2+,:/2006457,645nm,33OD645Fig.3InfluenceofconcentrationofZn2+onOD6453,a,001mgL-1,,OD645,,;001mgL-1,OD645,;005mgL-1,OD645,,3,645nm,Zn2+,645nm,,,:,:[1],.[J].(),1985,7():1-8.[2],,.[J].(),2005,27(2):170-175.[3],,.[J].(),2005,27(3):272-276.[4],.[J].,1997,16(2):26-29.[5],,,.[J].,2002,15(2):16-18,33.[6],.[J].,2002,21(5):500-506.[7],.[J].,2002,23(2):108-111.[8],,,.[M].:,2003:105-136.[9],,,.[J].,2004,23(5):593-604.[10]GeofferyMichaelGadd.Biosorption[J].ChemistryIndustry,1990,(3):421-426.[11]KaplanD,TadlerT.AlgalBiotechnology[M].London:Elsev-ierAppliedScience,1988:179.[12],,.[M].:,2001:144.[13].[J].,2000,19(1):35-37.[14],.[J].,2001,21(4):27-29.[15].()[M].:,2003:61-62.StudyonSpectrophotometryMeasurementofAlgaeGrowthinDianchiLakeDUANGang1,LIUXiao-hai1,GAOYun-tao2,DUGang2,TIEJin-gang2,WANGWei3(1.YunnanInstituteofEnvironmentalScience,Kunming650034,China;2.SchoolofChemistryandBioengineering,YunnanNationalitiesUniversity,Kunming650031,China;3.SchoolofChemistryandMaterialScience,YunnanUniversity,Kunming650031,China)Abstract:ThewatersamplewastakenfromDianchiLake.Afterfiltered,itwasinoculatedinMDMinglasshouse,andthechlorophyllofalgaewasabstracted.Theabsorbancevaluesofchlorophyllaweremeasuredatvariouswavelength,soalgaegrowthcouldbemeasuredbythecontentofchlorophyllawhichcouldbecalcu-latedbasedonchlorophyllaformula.Theexperimentresultsshowedthatabsorbancevalueswerestronginthewavelengthscopeof400~460nmand640~660nm.Theresultsweregoodbyspectrophotometrymeasure-mentofeffectsofZn2+onalgaegrowthinDianchiLakeundertheconditionofwavelength645nm.Keywords:DianchiLake;chlorophylla;algae;wavelength;absorbancevalue