ACL9000ClotCurveRevC5-XXXX

RevisionC5/2004PN9000PPT09/26/031ACL8000,9000,and10000™ClotCurvesandD-DimerReactionCurvesHemostasisTechnicalMarketingRevisionC5/2004PN9000PPT09/26/032ObjectivesReviewNephelometryDiscusshowclotcurvesaredevelopedReviewnormalclotcurvesUnderstandwhyabnormalclotcurvesoccurReviewabnormalclotcurvesRevisionC5/2004PN9000PPT09/26/033Whatisaclotcurve?RevisionC5/2004PN9000PPT09/26/034TiltTubeMethodvs.Instrumentation•OperatoraddsreagentsandincubatesVisuallydeterminesendpointorclotTiltTubeInstrumentation•TestisautomatedInstrumenttakesopticalreadingsUsesformulaoralgorithmtoconvertopticalreadingstoendpointRevisionC5/2004PN9000PPT09/26/035NephelometricOpticDetectionNephelometryisthemeasurementoflightscatteredbyaparticulatesolution.Photo-sensorLightscatteredto90degreesLightscatteredLEDat660nmRevisionC5/2004PN9000PPT09/26/036Algorithm:AformulaorprocessusedtoconverttherawopticalmeasurementsintoaclottimeDefinedintheTestDefinitionScreenSmoothesthedatatodisplayavisiblelineChecksforclotintegrityRawdatathatdoesnotpassallcriteriawillbedisplayedwithanerrorRevisionC5/2004PN9000PPT09/26/037ThresholdPlotLightScatterReading(S/R)*100BaselineTIME(Seconds)FirstThresholdSecondThresholdRevisionC5/2004PN9000PPT09/26/038LightScatterReadingTIME(Seconds)(S/R)*1002ndDerivativePlotMinimapeakMaximapeakRevisionC5/2004PN9000PPT09/26/039CentrifugalAnalysisOneLED19samplesmaybeprocessedinonerunSamplesandReagentsloadedintorotorsimultaneouslyBridgeallowsseparateincubationMixedbyCentrifugalforceRevisionC5/2004PN9000PPT09/26/0310ClotSignatureCurveCreationIncreasingLightScatterIncreasingTime(seconds)RevisionC5/2004PN9000PPT09/26/0311LightScatterReadingTIME(Seconds)AccelerationPlateau0(S/R)*100ThepartsofaClotCurveBaselineRevisionC5/2004PN9000PPT09/26/0312BaselineAccelerationPlateauRevisionC5/2004PN9000PPT09/26/0313AccelerationBaselinePlateauRevisionC5/2004PN9000PPT09/26/0314RevisionC5/2004PN9000PPT09/26/0315RevisionC5/2004PN9000PPT09/26/0316HowdoIknowwhenIhaveanabnormalcurve?RevisionC5/2004PN9000PPT09/26/0317FlaggingLimitsResultsthatarewithinthenormalrangeResultsthatareoutsideofnormalrangeResultsthatareoutsideoftestrangeResultsoutsidescalerangeNoflags*Bold***•BlackPrintoutComputer•Violet•Red•NoresultsRevisionC5/2004PN9000PPT09/26/0318ErrorCodesR–DataReductionErrorsE–InstrumentErrorsQ–QCErrorsQ–CalibrationErrorsQ–AnalyticalReferenceErrorsRevisionC5/2004PN9000PPT09/26/0319RevisionC5/2004PN9000PPT09/26/03RevisionC5/2004PN9000PPT09/26/0320RevisionC5/2004PN9000PPT09/26/0321ReactionCurveErrorsErrorcode5:Opticalfailure.Errorcode6:Firstthresholdnotpassed.Errorcode7:Secondthresholdnotpassed.Errorcode8:Deltatimebetweenthresholdsistoolong.Errorcode9:Initialslopeofreactionistoohigh.Errorcode10:Finalslopeofreactionistoohigh.Errorcode11:Finaldeltaofcurveistoohigh.Errorcode12:Maximumpeakof1stderivativeisbelowlimit.Errorcode13:Maximumpeakof2ndderivativeisbelowlimit.Errorcode14:Offseterror.RevisionC5/2004PN9000PPT09/26/0322RevisionC5/2004PN9000PPT09/26/03RevisionC5/2004PN9000PPT09/26/0323RevisionC5/2004PN9000PPT09/26/0324RevisionC5/2004PN9000PPT09/26/0325RevisionC5/2004PN9000PPT09/26/0326RevisionC5/2004PN9000PPT09/26/0327RevisionC5/2004PN9000PPT09/26/0328RevisionC5/2004PN9000PPT09/26/0329RevisionC5/2004PN9000PPT09/26/0330RevisionC5/2004PN9000PPT09/26/0331RevisionC5/2004PN9000PPT09/26/03RevisionC5/2004PN9000PPT09/26/0332RevisionAJune2003RevisionC5/2004PN9000PPT09/26/03RevisionC5/2004PN9000PPT09/26/0333RevisionC5/2004PN9000PPT09/26/0334RevisionC5/2004PN9000PPT09/26/0335RevisionC5/2004PN9000PPT09/26/0336D-DimerReactionCurveInterpretationRevisionC5/2004PN9000PPT09/26/0337InterpretingD-DimerReactionCurvesontheACL9000ImmunoAssay–reliesonformationofantigen-antibodycomplexestoaffectlighttransmissionPrinciple:LatexparticlescoatedwithamonoclonalantibodydirectedtowardstheD-DimerdomainAsantibodycomplexesagglutinate,theagglutinationwillaffecttheamountoflightabsorbedAnalyzedusingtheChromogenicChannel–measuresabsorbanceoflightwith405nmchannelRevisionC5/2004PN9000PPT09/26/0338RevisionC5/2004PN9000PPT09/26/0339RevisionC5/2004PN9000PPT09/26/0340RevisionC5/2004PN9000PPT09/26/0341RevisionC5/2004PN9000PPT09/26/0342RevisionC5/2004PN9000PPT09/26/0343RevisionC5/2004PN9000PPT09/26/0344RevisionC5/2004PN9000PPT09/26/0345RevisionC5/2004PN9000PPT09/26/03461.291.26RevisionC5/2004PN9000PPT09/26/0347AbnormalD-DimerReactionCurvesRevisionC5/2004PN9000PPT09/26/0348RevisionC5/2004PN9000PPT09/26/0349RevisionC5/2004PN9000PPT09/26/0350RevisionC5/2004PN9000PPT09/26/0351RevisionC5/2004PN9000PPT09/26/0352RevisionC5/2004PN9000PPT09/26/0353RevisionC5/2004PN9000PPT09/26/0354RevisionC5/2004PN9000PPT09/26/0355Y=mx+qY=3178(0)+133Y=133DDH=133(5)=665RevisionC5/2004PN9000PPT09/26/0356RevisionC5/2004PN9000PPT09/26/0357RevisionC5/2004PN9000PPT09/26/0358RevisionC5/2004PN9000PPT09/26/0359RevisionC5/2004PN9000PPT09/26/0360RevisionC5/2004PN9000PPT09/26/0361RevisionC5/2004PN9000PPT09/26/0362Anyquestions?RevisionC5/2004PN9000PPT09/26/0363ClotCurves(Reactioncurves)areagreattool.Thistechnolog