核酸电泳相关试剂、缓冲液的配制方法

技术官员村位于位于亚运城东部，主干道二以东石楼涌以西的地块，占地面积、m2，总建筑面积、m2，共包括地下室南区、地下室北区、地上部分1栋～12栋、服务中心、室外工程等多个单体工程。其中住宅面积m2，共12栋，1～7栋建筑层数为11层，8～12栋11层（局部复式顶层），首层局部架空，布置公建配套设施。integratedenergy,chemicalsandtextileYibincity,arethethreecorepillarsoftheindustry.In2014,thewuliangyebrandvalueto73.58billionyuan,thecity'sliquorindustrysliptostabilise.Promotingdeepdevelopmentofintegratedenergy,advancedequipmentmanufacturingindustry,changningdistrict,shalegasproductioncapacityreached277millioncubicmetres,builtthecountry'sfirstindependenthigh-yieldwellsandpipelinesinthefirstsection,theleadinfactoryproductionandsupplytothepopulation.2.1-3GDPgrowthfigure2.1-4Yibin,Yibincity,Yibincity,fiscalrevenuegrowth2.1.4topographyterrainoverallisSouthwest,North-EasternState.Lowmountainsandhillsinthecitylandscapeasthemainridge-and-Valley,pingbasmallfragmentednaturepictureforwaterandthesecondlandofthesevenhills.236metersto2000metersabovesealevelinthecity,lowmountain,46.6%hills45.3%,pingbaonly8.1%.2.1.5developmentofYibinlandscapesanddistinctivefeatureinthecenterofthecity,withlimitations,andspatialstructureoftypicalzonalgroup,2012-citiesinbuildingwithanareaofabout76.2km2.Fromcity-buildingsituation,oldtown-theSouthBankCenterconstructionislaggingbehind,disintegrationoftheoldcityisslow,optimizationandupgrading,qualitypublicserviceresourcesarestillheavilyconcentratedintheoldtowntogether.SouthbankCentrehasnotbeenformed,functionsoftheserviceareaspaceismissing.Meanwhile,peripheralgroupcentresservicewasweakandinadequateaccountingforcitydevelopment,suspiciouspatterncouldnotbeformed.Asregardstransport,withtheoutwardexpansionofcities,citieshavebeenexpanding,centripetalcitytrafficorganizationhasnotchanged,integratedenergy,chemicalsandtextileYibincity,arethethreecorepillarsoftheindustry.In2014,thewuliangyebrandvalueto73.58billionyuan,thecity'sliquorindustrysliptostabilise.Promotingdeepdevelopmentofintegratedenergy,advancedequipmentmanufacturingindustry,changningdistrict,shalegasproductioncapacityreached277millioncubicmetres,builtthecountry'sfirstindependenthigh-yieldwellsandpipelinesinthefirstsection,theleadinfactoryproductionandsupplytothepopulation.2.1-3GDPgrowthfigure2.1-4Yibin,Yibincity,Yibincity,fiscalrevenuegrowth2.1.4topographyterrainoverallisSouthwest,North-EasternState.Lowmountainsandhillsinthecitylandscapeasthemainridge-and-Valley,pingbasmallfragmentednaturepictureforwaterandthesecondlandofthesevenhills.236metersto2000metersabovesealevelinthecity,lowmountain,46.6%hills45.3%,pingbaonly8.1%.2.1.5developmentofYibinlandscapesanddistinctivefeatureinthecenterofthecity,withlimitations,andspatialstructureoftypicalzonalgroup,2012-citiesinbuildingwithanareaofabout76.2km2.Fromcity-buildingsituation,oldtown-theSouthBankCenterconstructionislaggingbehind,disintegrationoftheoldcityisslow,optimizationandupgrading,qualitypublicserviceresourcesarestillheavilyconcentratedintheoldtowntogether.SouthbankCentrehasnotbeenformed,functionsoftheserviceareaspaceismissing.Meanwhile,peripheralgroupcentresservicewasweakandinadequateaccountingforcitydevelopment,suspiciouspatterncouldnotbeformed.Asregardstransport,withtheoutwardexpansionofcities,citieshavebeenexpanding,centripetalcitytrafficorganizationhasnotchanged,三、核酸电泳相关试剂、缓冲液的配制方法50×TAEBuffer(pH8.5)组份浓度2MTris-醋酸，100mMEDTA配制量1L配制方法1.称量下列试剂，置于1L烧杯中。Tris242gNa2EDTA·2H2O37.2g2.向烧杯中加入约800ml的去离子水，充分搅拌溶解。3.加入57.1ml的乙酸，充分搅拌。4.加去离子水将溶液定容至lL后，室温保存。10×TBEBuffer(pH8.3)组份浓度890mMTris-硼酸，20mMEDTA配制量1L配制方法l.称量下列试剂，置于lL烧杯中。Tris108gNa2EDTA·2H2O7.44g硼酸55g2.向烧杯中加入约800ml的去离子水，充分搅拌溶解。3.加去离子水将溶液定容至lL后，室温保存。10×MOPS(3-吗啉基丙磺酸)Buffer组份浓度200rnMMOPS，20mMNaOAc，10mMEDTA配制量1L配制方法1.称41.8gMOPS，置于1L烧杯中。2.加约700mlDEPC处理水，搅拌溶解。3.使用2NNaOH调节pH值至7.0。4.再向溶液中加入下列试剂。1MNaOAc(DEPC处理)20ml0.5MEDTA(pH8.0)(DEPC处理)20ml5.用DEPC处理水将溶液定容至1L。6.用0.45m滤膜过滤除去杂质。7.室温避光保存。注：溶液见光或高温灭菌后会变黄。变黄时也可使用，但变黑时不要使用。溴乙锭(10mg/ml)组份浓度10mg/ml溴乙锭配制量100ml配制方法1.称量1g溴乙锭，加入到100ml容器中。2.加入去离子水100ml，充分搅拌数h完全溶解溴乙锭。3.将溶液转移至棕色瓶中，室温避光保存。4.溴乙锭的工作浓度为0.5g/ml。技术官员村位于位于亚运城东部，主干道二以东石楼涌以西的地块，占地面积、m2，总建筑面积、m2，共包括地下室南区、地下室北区、地上部分1栋～12栋、服务中心、室外工程等多个单体工程。其中住宅面积m2，共12栋，1～7栋建筑层数为11层，8～12栋11层（局部复式顶层），首层局部架空，布置公建配套设施。integratedenergy,chemicalsandtextileYibincity,arethethreecorepillarsoftheindustry.In2014,thewuliangyebrandvalueto73.58billionyuan,thecity'sliquorindustrysliptostabilise.Promotingdeepdevelopmentofintegratedenergy,advancedequipmentmanufacturingindustry,changningdistrict,shalegasproductioncapacityreached277millioncubicmetres,builtthecountry'sfirstindependenthigh-yieldwellsandpipelinesinthefirstsection,theleadinfactoryproductionandsupplytothepopulation.2.1-3GDPgrowthfigure2.1-4Yibin,Yibincity,Yibincity,fiscalrevenuegrowth2.1.4topographyterrainoverallisSouthwest,North-EasternState.Lowmountainsandhillsinthecitylandscapeasthemainridge-and-Valley,pingbasmallfragmentednaturepictureforwaterandthesecondlandofthesevenhills.236metersto2000metersabovesealevelinthecity,lowmountain,46.6%hills45.3%,pingbaonly8.1%.2.1.5developmentofYibinlandscapesanddistinctivefeatureinthecenterofthecity,withlimitations,andspatialstructureoftypicalzonalgroup,2012-c