搜索
12120043JournalofCelluloseScienceandTechnologyVol.12No.1Mar.20041004-8405(2004)01-0037-081*310027Q814.9ALaccase,EC1.10.3.2Phenolase1883YoshidaRhusvernicifern1894Bertrand[1]CoriolusversicolorPanusconchatusLiPMnPH2O2[2]H2O22003-09-08*1980~381211.159~390kDa10%~80%441Cu2+600nmCu2+Cu4+Cu4+[3]1.21.2.12501α[4~7]234S[8]5Fc-HFcRR=CH2OHCOOCH2Fc[9]61-2-(3,4-)1391.2.2Cu2+Cys-His[10]O2[11]2LMS2MM+PMDMPD[12]4012N-OHN=ON-2.12,2’---(3--6-)ABTSABTSABTSABTSdicationABTS3ABTSABTS[13]1412.21-HBT/HBTHBTHBTHBTABTSHBT/HBTHBTHBT/HBT(BT)[14]2.3N-N-NHANHANHANHANONHANHA[15]33.1/1990BourbonnaisPaice[16]ABTS5Kappa12.19.2ABTS1998Sealey[17]/ABTS/1-5%/ABTS35%/1-55%Panusconchatus39ABTSLQPKappa17.442124.4[18]3.2ABTSHBTCampos[19]TrameteshirsutaSclerotiumrolfsiiSclerotiumrolfsii30%Graca[20]/20min100%NovozymeDeniliteSDeniliteS[21]3.3BODCODKazuhito[22]2,4,5-2,4,6-2,4-Johannes[23]/ABTS/1-35%ABTS75%1-45%ReyesPilar[24]1-HiraiHirofumi[25]143/HBT66LMS242,00028,3006679,30014,7004LMSLMS[1].[J].,1992,11(3):26-27.[2]LawrenceY,JianY.Ligninase-catalyseddecolorizationofsyntheticdyes[J].WaterRes,1997,31(5):1187-1193.[3]NelsonD,MariaAR,AlessandroD,etal.Applicationsoflaccasesandtyrosinases(phenoloxidases)immobilizedondifferentsupports:areview[J].EnzymeandMicrobialTechnology,2002,31:907-931.[4]AktasN,CicekH.Reactionkineticsforlaccase-catalyzedpolymerizationof1-naphthol[J].BioresourceTechnology,2001,80(1):29-36.[5]LeontievskyAA,MyasoedovaNM,BaskunovBP,etal.Transformationof2,4,6-trichlorophenolbythewhiterotfungiPanustigrinusandCoriolusversicolor[J].Biodegradation,2000,11:331-340.[6],,.2,6-[J].,1992,7:940-944.[7]TetsuyaF,HiroyukiU,YoshikoT.DegradationofbisphenolAbypurifiedlaccasefromTrametesvillosa[J].BiochemicalandBiophysicalResearchCommunications,2001,284:704-706.[8],.[J].,1992,12(1):37.[9],.Fc-R[J].(),1996,42(4):443-448.[10],,,./O2[J].,2001,17(2):153-159.[11]ChristopherF.Thestructureandfunctionoffungallaccase[J].Microbiology,1994,140(1):19.[12]YuxingW,JianY.Laccase-catalyzeddecolorizationofsyntheticdyes[J].WaterRes,1999,33(16):3512-3520.[13]MajcherczykA,JohannesC,HuttermannA.OxidationofaromaticalcoholsbylaccasefromTrametesversicolormediatedbythe2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulphonicacid)cationradicalanddication[J].ApplMicrobiolBiotechnol,1999,51:267-276.[14]PotthastA,RosenauT,FischerK.Oxidationofbenzylalcoholsbythelaccasemediatorsystem4412(LMS)Acomprehensivekineticdescription[J].Holzforschung,2001,55(1):47-56.[15],,./[J].,2001,5:1-4.[16]BourbonnaisR,PaiceMG.DemethylationanddelignigicationofkraftpulpbyTrametesversicolorlaccaseinthepresenceof2,2’-azinobis-(3-ethylbenzthiazoline-6-sulpHonate)[J].AppliedMicrobiologyBiotech,1992,36:823-827.[17]SealeyJ,RagauskasAJ.Investigationoflaccase/N-hydroxybenzotriazoledelignificationofkraftpulp[J].WoodChemTechnol,1998,18(4):403-416.[18]YuHuisheng,WangCaihua,etal.ISWPCMontrealQue,Canada,1997.[19]CamposR,KandelbauerA,RobraKH.IndigodegradationwithpurifiedlaccasesfromTrameteshirsutaandSclerotiumrolfsi[J].JournalofBiotechnology,2001,89:131-139.[20]GracaMB,SoaresMT,PessoadeAmorim,etal.UseoflaccasetogetherwithredoxmediatorstodecolourizeRemazolBrilliantBlueR[J].JournalofBiotechnology,2001,89:123-129.[21].[M]..:2002.284-286.[22]KazuhitoI,MihoF,KazuoK,etal.PhenolicacidsaffecttransformationofchlorophenolsbyaCoriolusversicolorlaccase[J].SoilBiology&Biochemistry,2000,32:85-91.[23]JohannesC,MajcherczykA,HuttermannAH.DegradationofanthracenebylaccaseofTrametesversicolorinthepresenceofdifferentmediatorcompounds[J].AppliedMicrobiologyandBio-technology,1996,46(3):313-317.[24]ReyesPilar,PickardMichaelA,Vazquez-DuhaltRafael.Hydroxybenzotriazoleincreasestherangeoftextiledyesdecolorizedbyimmobilizedlaccase[J].BiotechnologyLetters,1999,21(10):875-880.[25]HiraiHirofumi,NishidaTomoaki,FujisawaMikihito.DegradationofpolyethyleneandNylon-66bythelaccase-mediatorsystem[J].JournalofPolymersandtheEnvironment,2001,9(3):103-108.AdvancesinResearchandApplicationofLaccaseMediatorSystemLURong,XIALi-ming(DepartmentofChemicalEngineeringandBioengineering,ZhejiangUniversity,Hangzhou310027,China)Abstract:Recentadvancesinstudiesonactionmechanismandapplicationoflaccasemediatorsystemarereviewedinthispaper.Maincatalyticreactionsoflaccaseoccurinactivecenterformedbycoppercations.Therateandefficiencyofcatalyticreactionareincreasedandtherangeofsubstratesisexpandedwhenredoxmediatorisincombinationwithlaccase.Thislaccasemediatorsystemhasbeenwidelyusedinpulpbleaching,textilebiofinishingandenvironmentalprotectionprocesses.Keywords:laccase;redoxmediator;application