我国黄淮和南方主要大豆育成品种家族产量和品质优异等位变异在系谱

作物学报ACTAAGRONOMICASINICA2009,35(2):191−202:[e-mail](863)(2006AA100104),(973)(2004CB7206,2006CB101708,2009CB118404),(30490250,30671266),(B08025)*(Correspondingauthor):,E-mail:[e-mail];Tel:[phone]:E-mail:[e-mail]():2008-05-14;Accepted(:2008-09-16.DOI:10.3724/SP.J.1006.2009.00191我国黄淮和南方主要大豆育成品种家族产量和品质优异等位变异在系谱中遗传的研究张军1,2赵团结1盖钧镒1,*1//,210095;2,256603:,19085SSR,16358-16111493-11138-2,,9324,91%36%13%31%2,,;,5,5,97,2.23,2.36,4.17,,,,,,:;;;InheritanceofEliteAllelesofYieldandQualityTraitsinthePedigreesofMa-jorCultivarFamiliesReleasedinHuanghuaiValleysandSouthernChinaZHANGJun1,2,ZHAOTuan-Jie1,andGAIJun-Yi1,*1SoybeanResearchInstituteofNanjingAgriculturalUniversity/NationalCenterforSoybeanImprovement/NationalKeyLaboratoryforCropGeneticsandGermplasmEnhancement,Nanjing210095,China;2BinzhouVocationalCollege,Binzhou256603,ChinaAbstract:Itiscommonlyunderstandthatmolecularmarkershaveprovidedanopportunityforplantbreederstotracegeneticrelationshippreciselyamongreleasedcultivars,whileitcanbedoneonlyroughlythroughpedigreeanalysis.Inthepresentstudy,163releasedsoybeancultivarsofthefivemajorfamilypedigreesinHuanghuaiValleysandSouthernChina,with58-161,Xudou1,Qihuang1,Nannong493-1,andNannong1138-2astheirrespectivepedigreeancestor,wereanalyzedfortransitionofeliteallelesofyieldandqualitytraitsineachfamilypedigreebasedontheassociationanalysisbetween85SSRlociandagronomictraitsin190releasedcultivarsinthesameregion.Twobestallelesofeachofthenine,three,twoandfourmajorloci,explaining91%,36%,13%,and31%oftotalphenotypicvariationofthefourtraits,respectively,weretracedfortheirtransitioninthefivecultivarpedigrees.Itwasfoundthateachpedigreeancestorhaditsownelitealleleswhichtransitedtoitsprogenies,butsomeofthemmightbelostduringtheprocess.Thefivefamilypedigreestendedtosharealltheeliteallelesbutwithdifferentfrequencydistributionsduetodiverseparentalmaterialsusedinthepedigrees.Thecultivarsinthepedigreeshaddifferentnumberofeliteallelesforyield,wherethehighesteliteallelenumbercontainedinacultivarwassevenbutnotthefullof9andtheaveragewasonly2.33,indicatinggreatyieldpotentialinrecombinationandaccumulationofeliteallelesforfuturebreeding.Undertheex-19235perimentconditions,theaverageyieldofhigh-yieldingcultivarswas2.36timesofthatoflow-yieldingcultivarswhiletheaverageeliteallelenumberoftheformerwas4.17timesofthatofthelatter,butthecompositionofeliteallelesamongthehigh-yieldingcultivarswasquitedifferent.Ontheotherhand,unusualcasesofsomecultivarswithhighyieldbutlesseliteallelesandsomewithlowyieldbutmoreelitealleleswerealsoobserved.Itissuggestedforbreederstoconservecarefullytheoldcultivarsforfuturebreedingsincetheymighthavesomespecificeliteallelesintheirgenome.Keywords:Soybeanreleasedcultivar;Familypedigree;Eliteallele;Inheritance,,[1-5],QTL[6-10],[11]85SSR19011,7,45136()11QTL,22()QTL,43()2192320051300,618,,,,,5,(2;2),,1材料与方法1.158-16111493-11138-25(1),200516013015050305()()37.28(56)17.88(12)21.16(29)9.95(15)12.77(21)A29551-83,[6]8473452220(2)1.22005,400(),,2,3,2.0m,0.5m20062005400()190(),314×14,5,2.0m,0.5m,10cm3,18d,212.25m2,,(INFRATEC1255Food&FeedAnalyzer),20052006131.385SSR(190)2,2;,2[11]9,3(Sat_251,Satt365,Satt311)2,1(Satt365)QTL;6(Satt347,Satt443,Sat_299,Satt665,Sat_312,Satt436);2:193表1品种编号和名称Table1CodesandnamesofcultivarsusedinthestudyCodeCultivarCodeCultivarCodeCultivarCodeCultivar158-161421Qihuang1838Kexin81242Gongdou2212Yudou124310Puhai10846Kexin61254Gongdou4321Dishen21445Qihuang585Pixianruantiaozhi1265Gongdou5422Dishen22455Jidou5861099Shangqiu10991276Gongdou6516Yudou164610Ludou10877608Shangqiu76081282Hedou2619Yudou19476Mengqing6882Yudou21293Hedou3721Yudou21482Sidou2893Yudou31302Huaidou2822Yudou22493Lindou3908Yudou81315Nandou5984-5He84-5501Ludou19110Yudou101321138-2Nannong1138-21020Huadou20512Ludou2925Yuejin5133128Nannong1281123Yudou23521Wandou193Lichengxiaoliqing134242Nannong2421224Yudou24534Ludou4946Zaoshu6135493-1Nannong493-11330Youbian30541Luning19590007Zheng9000713673-935Nannong73-9351431Youbian31556huaidou69692116Zheng9211613786-4Nannong86-41524Wandou24566Wandou697107Zhengjiao10713887C-38Nannong87C-38161Fudou1579Wandou9989525Zhengjiao952513988-31Nannong88-311728Yudou28581Weimin199135Zhengzhou13514088-48Nannong88-481823Jindou23595Wenfeng510019Zhongdou1914199-10Nannong99-101923Juxuan23606Wenfeng61014Kexin414299-6Nannong99-62036Kefeng36617Wenfeng710213Zhonghuang131431Ningzheng12137Kefeng376210Xudou1010314Zhonghuang141442Qiandou22253Kefeng536319Zhonghuang1910410Qihuang101453Qiandou323135Xudou1356415Zhonghuang151052Qichadou21466Qiandou6248Kexi8656Kefeng610624Zhonghuang24147Shanghailiuyuebai2526Zhongdou26661Xudou110725Zhonghuang251487209Su72092626Yudou26672Xudou21084Zhonghuang41491Sudou12725Yudou25683Xudou31097Zhonghuang71503Sudou32818Zaoshu18697Xudou71107327-118Zhou7327-11815118-6Suxie18-62913Wandou13708Xudou811111Zhoudou1115219-15Suxie19-153029Yudou29719Xudou911212Zhoudou121531Suxie13112Ludou12724Yandou411371061544-1Suxie4-13216Wandou16734Huaidou4114Aiji