IUE(胚胎电转)

IUEintroFigure1Anatomicalorganizationofthedevelopingforebrain.(A)SchemaofasagittalsectionthroughthebrainofanE12.5mouseshowingthemainsubdivisionsoftheforebrain,thediencephalonandthetelencephalon.Inthetelencephalon,thepalliumisdepictedinlightergraythanthesubpallium.(B)SchemaofatransversalsectionthroughthetelencephalonofanE12.5mouse,indicatingsomeofitsmainsubdivisions.LGE,lateralganglioniceminence;MGE,medialganglioniceminence;POA,anteriorpreopticarea.-----OscarMarin,2003Neurosci-----SusanK.McConnell,1995NeuronFigure2Mechanismsregulatingradialmigrationinthecerebralcortex.Shownisaschematicrepresentationofradiallymigratingneuronsinthedevelopingcortex,wheretheventricularzoneistothebottomandthemarginalzoneistothetop.Moleculesinvolvedinradialmigrationareindicatedinrelationtotheirfunction.-----OscarMarin,2003Neurosci-----AmitabhGupta,2002Nature-----EmiliePacary,2012JournalofVisualizedExperimentsSurgical-----RenpingZhou,2013NeuralDevelopment:MethodsandProtocols,MethodsinMolecularBiologyPreparationoftheskin(a–d)FourcontinuousstepstopreparetheskinforfurtherembryomanipulationExposureoftheembryos(a–d)FourcontinuousstepstoexposetheembryosPreparationofmicropipettetip(a–d)ThreecontinuousstepstopreparethemicropipettetipforinjectionInjectionofDNAandelectroporation(a–c)ThreecontinuousstepstoinjectDNAtoembryonicbrainventriclesfollowedbyelectroporation(d)Sendingbackofembryos(a–d)FourcontinuousstepstosendembryosbackSuturingofmuscleandskin(a,b)Twostepstoclosetheabdominalwall(A)SchematicsofexperimentalproceduresofpiggyBac(PB)–IUEinMyD88flox/floxmice(left)andrepresentativeimageofhippocampalslicesfromPB-IUEmice(right).Bar,200µm.-----YiShen2016J.CellBiol.thankyou!