厌氧氨氧化菌的生物特性及CANON厌氧氨氧化工艺_王亚宜

34620146ActaScientiaeCircumstantiaeVol．34No．6Jun．2014No．5137837051178325No．12QH1402400SupportedbytheNationalNaturalScienceFoundationofChinaNSFCNo．5137837051178325theFundamentalＲesearchFundsfortheCentralUniversitiesandtheShanghaiScienceandTechnologyDevelopmentFundsNo．12QH14024001977—[e-mail]*BiographyWANGYayi1977—[e-mail]*CorrespondingauthorDOI10．13671/j．hjkxxb．2014．0565．2014．CANONJ．3461362-1374WangYYLiLMaXetal．2014．Bio-characteristicsofanammoxbacteriaandCANONanammoxprocessJ．ActaScientiaeCircumstantiae3461362-1374CANON1*111211．2000922．2000402013-09-182013-12-162014-01-01ANaerobicAMMoniumOXidationANAMMOX．、“”．．CANONCompletelyAutotrophicNitrogenremovalOverNitrite．CANON．CANON0253-2468201406-1362-13X703ABio-characteristicsofanammoxbacteriaandCANONanammoxprocessWANGYayi1*LILi1MAXiao1LINXimao1PANMianli2DAIXiaohu11．StatekeylabofpollutantcontrollingandresourcereuseCollegeofEnvironmentalScienceandEngineeringTongjiUniversityShanghai2000922．CenterforPharmacologicalEvaluationShanghaiInstituteofPharmaceuticalIndustryShanghai200040Ｒeceived18September2013receivedinrevisedform16December2013accepted1January2014AbstractANAMMOXANaerobicAMMoniumOXidationreferstooxidizingammoniausingnitriteaselectronacceptorunderanoxicconditions．Thisprocessiscatalyzedbyauniquegroupofobligatoryanaerobicmicrobescalled“anammoxbacteria”．AsmembersofthePlanctomycetesphylumofBacteriaanammoxbacteriahaveadistinctivecellstructurewhichdiffersgreatlyfromthecommonprokaryocytes．Mostimportantlyanammoxplaysanimportantroleinthenitrogencycleandpossessagreatpotentialinwastewatertreatment．Anammoxprocesscombinedwithpartialnitrificationisideallysuitabletotreathigh-strengthammoniastreamswithlowornoorganicmatter．Intheone-stageCompletelyAutotrophicNitrogenremovalOverNitriteCANONprocessaerobicandanaerobicammoniaoxidizingbacteriacooperatetoremoveammoniainoneoxygen-limitedreactorwhichsavesthelandoccupationbutbringsaboutachallengeinhowtocontrolthefunctionalbacteriacommunity．Inthiscontributionbio-characteristicsofanammoxbacteriaandCANONprocessarereviewed．KeywordsanammoxpartialnitrificationCANONwastewaternitrogenremoval1Introduction2060．-．NH+4NO－3①AOBNO－2②6CANONNOB．/．CO2．．short-cutnitrification-denitrificationPengandZhu2006．25%40%．2090MulderKuenen———AnAOBMulderetal．1995Strousetal．1999aKuenen2008．．AnAOBpartialnitrificationAOBAnAOBAOB．62．5%CO2CO2．2Bio-characteristicsofanammoxbacteria2．11986GistBrocadeMulder．Mulder“”ANaerobicAMMoniumOXidationANAMMOXMulderetal．1995．vandeGraafvandeGraafetal．1995AnAOBvandeGraafetal．1996．AnAOB．1999Strous1999a《Nature》density-gradientcentrifugationAnAOB16SrＲNAAnAOBPlanctomycetesAnAOB．AnAOB3①AnAOB．AnAOBFuerstandSagulenko2011．AnAOBKartaletal．20112013②AnAOB1．AnAOBArrigo2005LamandKuypers2011．③AnAOB．．、．1Fig．1Thenitrogencycle2．2AnAOB363134AnAOBvandeGraafetal．1996．AnAOBAnAOB．SBＲAnAOBStrousetal．1998．SBＲUASBImajoetal．2004Nietal．2010Schmidtetal．2004、ＲotatingbiologicalcontactorＲBCEglietal．2001AnAOB．Strous1998SBＲAnAOBNH+4+1．32NO－2+0．066HCO－3+0．13H+→1．02H2+0.26NO－3+2．03H2O+0．066CH2O0．5N0．15biomass12～3①1∶1ATP②CO2ATP．NH+4+NO－2=N2+2H2OΔG0'=－357kJ·mol－120．28NO－2+0．066HCO－3=0．26NO－3+0．066CH2O0．5N0．253N2NH+4NO－2．15NNH+4NO－2N214N15N．AnAOBN2NNO－3NO－2．massspectrometryvandeGraafetal．1997N214N14N14N15N15N15NAnAOB．2．35genera10speciesAnAOBBacteriaPlanctomycetesBrocadiales．5①BrocadiaStrousetal．1999aKartaletal．2008Oshikietal．2011AnAOB———Gist－Brocades3B．anammoxidansB．fulgidaB．sinicaB．anammoxidansAnAOB②KueneniaStrousetal．2006JohannesGijsbrechtKuenenK．stuttgartiensis③AnammoxoglobusKartaletal．2007bA．propionicus④JetteniaQuanetal．2008Huetal．2011MikeS．M．JettenJ．asiatica⑤ScalinduaSchmidetal．2003Woebkenetal．2008vandeVossenbergetal．20134S．brodaeS．sorokiniiS．wagneriS．profunda．2．4AnAOB800nm1100nm．AnAOB．AnAOB7～22dKartaletal．2013densitygradientcentrifugationStrousetal．1999aAnAOB99．6%2001．AnAOB．outermembrane．2aLindsayetal．2001．AnAOB32b①paryphoplasm②riboplasm③anammoxosome．AnAOB．AnAOB2b．K．stuttgartiensisAnAOBStrousetal．46316CANON2006AnAOB．．2aPirellulastaleyibcFig．2athecellstructureofPirellulastaleyioftypicalPlanctomycetesbthecellstructureofanammoxbacteriaremaintobeverifiedcladderanelipidandanammoxosomemembrane．AnAOB．AnAOB“CandidatusBrocadiafulgida”“CandidatusAnammoxoglobuspropionicus”PHApoly-PvanNiftriketal．2008．．2b．NH+4+NO－2→N2+2H2OAnAOBNiftriketal．2004．AnAOBladderanelipidDamstéetal．20022cN2H4H+．2．5、．NH3→NO－2NH2OHNO－3→N2NO－2、NON2O．．VandeGraaf1997NH2OHAnAOB14NH+415NH2OHNO－21415N2N2H43NH2OHN2H4．3①NO－2NH2OH②NH2OHNH+4N2H4③N2H4N2．NO－2NH2OHNH2OH→NO－2．HAONH2OHN2H4N2Hooperetal．19973HAO．2N2H4、NorN—N．3NH2OHN2H4Kartaletal．2008Fig．3ProductionofhydrazinebyanammoxbacteriaaftertheadditionofhydroxylamineKartaletal．2008563134K．stuttgartiensisNO－2NH2OHcd1NirSStrousetal．2006NONONH2OH．Strous2006AnAOB4．NON2H4NO－2+2H++e－=NO+H2OE'0=+0．38V4NO+NH+4+2H++3e－=N2H4+H2OE'0=+0．66V5N2H4=N2+4H++4e－E'0=－0．75V6NH+4+NO－2=N2+2H2OΔG0'=－357kJ·mol－174NirHZSHDHccbc1bc1QQarFig．4ThecatabolismmodelofanammoxbacteriaNirnitritereductaseHZShydrazinesynthaseHDHhydrazinedehydrogenase