©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.:2009-06-22:(1981-),,2006:,,,,E-mail:songjinghui2002@yahoo.com,010050,3(,010050):,,;:;;:R378.992:A:1004-2113(2009)04-0431-05RESEARCHPROGRESSIONOFTHEMICROECOLOGYOFVAGINALACTOBACILLUSZHENGJing-jing,SONGJing-hui(DepartmentofGynecologyandObstetrics,AffiliatedHospital,InnerMongoliaMedicalCollege,Hohhot010050China)Abstract:VaginalLactobacillusisanimportantgroupofbeneficialbacteriawhichmaintainthebalanceofvaginalfloraanddefensegenitaltractinfections.StudyshowthatvaginalLactobacilluscanmaintainvaginalmicrobiotathroughthemechanismofcompetitionforadhesion,antimicrobialpropertiesandstimulationofimmunitydefense.Thispaperreviewsthemechanismofimmunitydefenseandapply2ingprospectioninLacticacidbacteriainvagina.Keywords:Lactobacillus;vaginalmicroflora;florabalance(Lacticacidbacteria,LAB)()[1],,[23],,,,,50,,[4],,,,,13420098314©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.:1857,LouisPasteur1873,JoserhListerBacteriumlactis1891,AlbertDoederlein,Doederlein,,,,(1928StanleyThoms),,,60,Morris219,,,,1973,,,80%90%,(107108CFU/mL)2001Kilic[5],Alexander[6]IL14032002Pavlova[7],2004,[8]6037,,,,,,,2007Witkin[4],,,,,,,,,16,H2O2[9]Savage,,,,,,,2,[10],,,,,,2.1,[11],,Boris,[12],,2.22.2.1pHpH318414,,[13]234ActaAcadMedNeiMongolAug.2009Vol.31No.4©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[14]2.2.2H2O2H2O2,H2O2,H2O2[15],EschanbachH2O2BVH2O2[16],H2O2,H2O2[17],H2O2H2O22.2.3,[18],[19]Valraeds15,1414140kpa,,-,75,,,2.2.4,,,,2.2.5,,,,,,,2.3,,[2021](IL-12IFN-TNF-,TGF-IL-10)TB,,,33.13.1.1,,,,[22]3.1.2,,,,,,,,APIEnterotubePhpMIS(MIDI)VITEKBiologAPI,15,20,550,,3.2-,PCR16SrDNA,16SrDNA33420098314©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.:16SrRNA(PCR);(DGGE)[23];(PF2GE)[24];16SrRNA(RFLP)[25];(RAPD);(FISH);(AFLP);DNA(PAPD)[26],,,,44.1,,50,?H2O2??????,,4.2,,,,,,,,,,,,,,4.3,,,,CIN,H2O2[27],HIV[28],LcrispatusL.jensenii[29],L.acidophilusL.crispatusL.delbrueckiiBV,,,,,,,[1],.[J].,2007;19(1):118-120[2]PollmanB.TransportersandtheirrolesinLABcellphysi2ology[J].AntonievanLeeuwenhoek,2002;82(8):147-164[3],.[J].,2006;18(4):328-330[4]WitkinSS,LinharesLM,GiraldoP.Bacteriafloraofthefe2malegenitaltract:functionandimmuneregulation[J].BestPractice&ResearchClinicalObstertricsandGynaecology,2006;21(3):347-354[5]KilicAO,PavlovaSI,AlpayS,etal.ComparativeStudyof434ActaAcadMedNeiMongolAug.2009Vol.31No.4©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[J].Clinicaianddiagnosticla2boratoryimmunology,2001;8(1):31-39[6]Alexander,BolotinPW.TheCompleteGenomeSequenceofthelacticacidbacteriumlactococcuslactisssp.lactisIL1403[J].GenomeResearch,2001;11(4):731-753[7]PavlovaSI,KillicAO,KilicSS,etal.Geneticdiversityofvaginallactobacilliformwomenindifferentcountriesbasedonthe16SRNAgenesequences[J].ApplMicrobiol,2002;92(3):451-459[8].[D].,2004:52-53[9]VallorAC,AntonioMA,HawesSE,etal.Factorsassociat2edwithacquisitionof,orpersistentcolonizationby,vaqinallactobacilli:roleofhydroqenperoxideproduction[J].JIn2fectDis,2001;184(10):1431-1436[10].[J].,2005;12(5):5-6[11]HeinM,PetersenAC,HelmigRB,etal.Immunoglobulinlevelsandphagocytesinthecervicalmucusplugattermofpregnancy[J].ActaObstetGynecolScand,2005;84(8):734-742[12]McGroartyJA,TomeczekL,PondDG,etal.Hydrogenperoxideproductionbylactobacillusspecies:correlationwithsusceptibititytothespermicidalcompoundnonoxynol-9[J].JInfectDis,2001;165(6):1142-1144[13]SchallerM,KortingHC,BorelliC.Candidaalbicans-se2cretedasparticproteinasesmodifytheepithelialcytokineresponseinaninvitromodelofvaginalcandidiasis[J].In2fectImmun,2005;73(5):2758-2765[14]VoravuthikunchaiSP,BilasoiS,SupamalaO.Antagonisticactivityagainstpathogenicbacteriabyhumanvaginallac2tobacilli[J].Anaerobe,2006;12(5-6):221-226[15]SethiS,DasA,SharmaM.InhibitionofGardnerellavagi2nalibylactobacilli[J].IntJGynaecolObstet,2006;93(2):158-159[16],.[J].,2007;8(2):150-152[17],,,.[J].,2006;35(3):310-313[18],,,.[J].,2004;26(2):145-147[19]McFarlandLV.Normalflora:diversityandfunctions[J].MicrobialEcolHealthDis,2000;12(4):193-207[20].[J].,2005;19(4):51-54[21],,,.[J].,2007;19(4):399-400[22].[J].,2008;37(4):508-510[23]TabascorR,PaarupT,JanerC,etal.Selectiveenumera2tionandidentificationofmixedculturesofStreptococcusthermophilus,Lactobacillusdelbrueckiisubsp.bulgari2cus,L.acidophilus,L.paracaseisubsp.paracaseiandBifidobacteriumlactisinfermentedmilk[J].IntDairyJ,2007;17(9):1107-1114[24]Vancanneytm,HuysG.Intraspecificgenotypiccharacter2izationoflactobacillusrhamnosusstrainsintendedforpro2bioticuseandisolatesofhumanorigin[J].ApplEnvironMicrobiol,2006;72(8):5376-5383[25]BlaiottaG,Fuscov,ErcoliniD,etal.Diversityoflactoba2cillusstrainsbasedonpartialHSP60genesequencesanddesignofPCR-RFLPassa