叶黄素酯和叶黄素稳定性的研究

27120072ChemistryandIndustryofForestProductsVol.27No.1Feb.2007:2005-07-13:(BG2006319):(1976-),,,,,;E2mail:[e-mail]3:,,LIDa2jing1,2,3,23,1(1.,150040;2.,210014;3.,150040):,,0.5h,0.35%,;,,,;,,,;,;Fe3+Fe2+Cu2+,,Zn2+Mg2+Ca2+K+Na+;H2O2;Na2SO3,VC,VC:;:TQ91;Q562;O629.4:A:0253-2417(2007)01-0112-05StabilityofLuteinandItsEstersLIDa2jing1,2,FANGGui2zhen3,LIUChun2quan2,YUJia2peng1(1.FoodSciencesandEngineeringDepartment,CollegeofForestry,NortheastForestryUniversity,Harbin150040,China;2.InstituteofApplicationinAtomicEnergy,JiangsuAcademyofAgriculturalSciences,Nanjing210014,China;3.CollegeofMaterialScienceandEngineering,NortheastForestryUniversity,Harbin150040,China)Abstract:Stabilitiesofluteinanditsestersagainstlight,heat,acid,alkali,metalions,oxidantandreductantwerestudied.Resultsshowedthatbothluteinandluteinesterswereunstableagainstlight,andstableindarkness.Theirpreservationratewas0.35%towardoutdoorsunlightfor0.5hwhiletheywerepreservedconstantlyindarkness.Theywereslightlystableagainstheatbuttheirdegradationcouldbeacceleratedwithdecreasedpreservationrateunderlongtimeandhightemperature.LuteinesterwasmorestabletowardlightandheatthanluteinunderthesameconditionandesterificationofOHgroupwithfattyacidsmightstabilizeluteinagainstheatandlight.TheywereunstablewithdecreasedpreservationratetowardFe3+,Fe2+,Cu2+andstrongacidwhilestableagainstZn2+,Mg2+,Ca2+,K+,Na+,alkaliandNa2SO3aswellasslightlystabletowardH2O2.ItwasnotedthatVCcoulddegradesignificantlyluteinestersandlutein,inwhichabsorbanceofluteinestersdecreasedmorequicklythanthatoflutein.Keywords:luteinesters;lutein[1],,,,[2],[3],,[4]Achmad1,:113[5],pH,,,11.1:CO2;(90%):,;,;,;,:,,,,,,,,,,,,;VC1.21.2.1,,[6],0.51369122472120240h(446nm)1.2.2,,25406080100,123456h,(431nm)1.2.3pH,(91,)[7],pH114,,24d,1.2.4,Mg2+()Ca2+()Fe3+()K+()Cu2+()Fe2+()Na+()Zn+(),0.5g/L,14d,1.2.5H2O2,0.3%0.6%0.9%1.2%13624h,1.2.6Na2SO3VC,0.61.22.44.8g/L,13624h,1.2.7=/100%22.111Table1Effectsoflightonthestabilityofluteinestersandluteinsamples1)conditionsabsorbanceatdifferenttime0h0.5h1h6h12h24h72h120h240hluteinestersA0.8530.8490.8570.8540.8530.8540.8640.8700.854B0.8530.8460.8380.7710.7470.7210.3320.0200.008C0.8530.003-------luteinA0.8550.8550.8540.8520.8500.8450.8510.8430.842B0.8550.8550.8420.7580.7210.7110.4420.1080.007C0.8550.003-------1)A.dark;B.indoordaylight;C.outdoorsunlight114271.2.7,240h,98.48%;24h84.52%,83.16%,240h0.94%,0.82%;0.5h0.352%,0.351%:,,,[7],,2.225406080100,22Table2Effectsoftemperatureonthestabilityofluteinesterandlutein/htimeabsorbanceofluteinesters25406080100absorbanceoflutein2540608010000.7130.7130.7130.7130.7130.7220.7220.7220.7020.70210.7130.7000.6960.6880.6790.7150.7230.7220.6750.66920.7060.6900.6880.6820.6680.7230.7120.7100.6670.65940.7130.6920.6840.6760.6700.7240.7040.6980.6440.64060.7160.6890.6690.6750.6570.7100.7050.6940.6310.6142,6h,98.34%;4096.63%,97.65%;6093.83%,96.12%;801h,96.49%,96.15%,6h94.67%,89.89%;1001h,95.23%,95.30%,6h92.14%,87.46%,,,Achmad[5],2,802.3pHpH11424d11pH(a)(b)Fig.1EffectsofpHvalueonthestabilityofluteinesters(a)andlutein(b)1,(pH14),4dpH248.42%,47.99%;pH380.70%,71.56%,,pH1112,,,,pH1,:115[6]2.4(0.5g/L)14d33Table3Effectsofmetalionsonthestabilityofluteinestersandluteinsamplesionsabsorbance0d1d4dluteinestersNa+0.7510.7420.745Cu2+0.8990.7260.713Zn2+0.7880.7740.756Ca2+0.7590.7510.754Fe3+0.7930.7950.669Fe2+0.8460.7350.695Mg2+0.7680.7590.758K+0.8340.7710.763luteinNa+0.7200.7090.697Cu2+0.7590.6760.610Zn2+0.8000.7230.789Ca2+0.7240.7180.712Fe3+0.7630.6530.619Fe2+0.8490.7240.690Mg2+0.7450.7390.742K+0.7780.7680.759Fe3+Cu2+Fe2+4d,,Ca2+K+Na+;Mg2+98.70%99.60%,Fe2+82.15%81.27%,Fe3+84.36%81.27%,Cu2+79.31%80.37%;Zn2+Ca2+K+Na+95.94%98.62%99.34%98.34%91.49%97.56%99.20%96.80%:Fe3+Fe2+Cu2+,;Zn2+Mg2+Ca2+K+Na+2.544,H2O20.3%,24h96.35%99.46%;0.6%96.08%97.45%;0.9%,95.10%96.62%;1.2%,94.91%95.82%,,,4H2O2Table4EffectsofH2O2onthestabilityofluteinestersandluteinH2O2/%H2O2volumepartluteinesters0h1h3h6h24hlutein0h1h3h6h24h00.7210.7230.7220.7240.7240.7460.7460.7390.7450.7450.30.7120.7080.7000.6980.6860.7400.7480.7430.7450.7360.60.7140.7110.7080.7030.6860.7440.7360.7290.7320.7250.60.7140.7100.7000.6970.6790.7390.7320.7230.7240.7141.20.7080.7070.6950.6930.6720.7410.7330.7250.7270.7102.65116275Na2SO3VCTable5EffectsofNa2SO3andVConthestabilityofluteinestersandluteinreductants/(gL-1)massconcn.luteinesters0h1h3h6h24hlutein0h1h3h6h24hNa2SO300.7120.7230.7230.7250.7160.7310.7400.7440.7440.7340.60.7070.7090.7090.7090.7030.7120.5940.5700.5690.5311.20.7060.7070.7090.7100.7000.7150.5780.5390.5320.4712.40.7090.7200.7200.7060.6850.6740.3580.1470.1290.0944.80.7030.7040.7070.7020.6960.6770.3500.1300.1110.084VC00.7650.7610.7640.7580.7640.7590.7550.7530.7560.7640.60.7660.7630.7690.7640.7660.7540.7490.7460.7500.7491.20.7710.7690.7730.7680.7710.7500.7440.7350.7320.7302.40.7840.7660.7670.7580.7430.7530.7400.7300.7220.6724.80.7930.7900.7980.7900.7720.7690.7540.7460.7360.6615,Na2SO32.4g/L,24h96.61%94.77%;Na2SO34.8g/L99.00%97.35%,Na2SO3,Na2SO35,VC2.4g/L,24h13.95%89.24%;VC4.8g/L12.41%85.95%,VC,,3:,,;,,;,,;,;Fe3+Fe2+Cu2+,Zn2+,Mg2+C