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275Vol127,No15199910CHINADAIRYINDUSTRYOct.,1999EffectofHighPressureSterilizationontheQualityofDairyProductsWangJiaqiHouLihong(150030)(430070)(InstituteofAnimalProductProcessing,NortheastAgri.Univ.)(Middle-ChinaAgri.Univ.)LuoChengxiang(150030)(InstituteofAnimalProductProcessing,NortheastAgri.Univ.):,:;Abstract:Thehighpressuretreatmentisacoldsterilizationtechnique.Itisappliedinfoodindustryinrecentyears.Thisarticlereviewsforhighpressuretechnologyanditseffectonmicroorganismandcom2ponentinmilk.Keywords:highpressuresterilization;milkquality:TS252.41:A:1001-2230(1999)05-0020-06090,,100MPa,,,:(1)MPa,,,,,(2),,(3),,(4)1,,,(100200MPa),;,,,1999-08-10(1972-),,,.©1995-2003TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.100MPa,,,300400MPa;;,;,1,[1],2025,200300MPa,60min;340400MPa,50min,(LPLT)(HPST)(UHP)(1993):LPLT400MPa1020min;HPST600MPa12min;UHP600MPa1min,(),,,4560600MPa()47,400MPa,(1991)13,-2020,200MPa,2012,-20;(-205),,-205,,-205,;;,,,,1[1]/MPa/min/20020-2040010253003040600102529010254001025()3002060()3002060()4502060()600406020020-2020020-2020020-2020020-2040010253001025300102530020-2060010252006040200180405003035600840-3404086020252043066020255786805-204306602025()971025()2001440402LeChatelier,,,,,pH(0.05%0.2%),(10%),12199910©1995-2003TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.,2.120,(0600MPa),(Hydrodynamicdia2materdistribution)1(a),1(a),0200MPa,:200450MPa,,,,;450MPa,,GauchcronF[2](4,20,40),(0450MPa),,(2(b))2(b),4,250MPa,;40,0250MPa,,:250nm50nm;450600MPa,,20,(a)(b)(c)(d)1[3](a);(b);(c);(d)2.2GaucheronF[2]ANS(8-aniliao-1-na2phyalenesulphonate),2(a)2(a),ANS,Fmax,Fmax(),HayakawaI[4](BSA),ANS,BSA,BSA,(a)22275©1995-2003TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.(b)2a.;b.2.3(Skimmilkul2tracentifugedpellet,SMUP)SMUP77000g2h,SMUP33,SMUP2.0L2.4g/g0250MPa;420,250600MPa,SMUP(2.42.5g/g);40,250600MPa,SMUP(2.452.30g/g),,,;,,,AnenaSGCreamerLK[5],3SMUP4;20;402.4-(-Lactoglobulin,-Lg)-,-Lg(450MPa)()DumayEM[6],-Lg(2.5%)(5%),-Lg,;5%-Lg,,-LgpH7.0,[7]:,pH7.58.0,;,pH6.0,-LgI(StrandsI)450MPa(25),15min-Lg(pH=7.0),pH6.0,-Lg,,-LgI,-Lg[8]-Lg,pH(2.0)-Lg(0300MPa)-Lg150MPa-Lg,(300MPa),(4)4--;-;-32199910©1995-2003TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.2.5(No-CasceinProtein,NCP),-Lg(5),-(-Lactobumin,Lb)(5%)NakamuraT[9]10%-LgLb2S2S2:Lb42S2S2,-Lg,Lb-Lg,(BSA)BSA2SH172S2S2,,,,BSA5NCP-LgNCP;-Lg2.6[10]:10%,IgG500MPa,pH7.0,400MPaIgGIgG,IgG2.7Ca,PCa,P:0250MPa,Ca,P(2025mg/kg);250600MPa,Ca,P,Ca,P,,GaucheronF[2]Ca,P,2.8,,,20%50%317;DeWitKlarenbeek(1981)(5%)10%-Lg(pH=6.7)Tmax()12DumayEM[6]5%2.5%-Lg(H44%27%)[10]IgG,50%IgG,6,:,,,,6IgG200MPa;400MPa;500MPa;700MPa42275©1995-2003TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.33.1,Rayleigh(6),(Eilcr),,,,11,3.2Desobry-BanonS[3]ShibauchiY[12],,,150MPa;230MPa,145s122s;150MPa;230670MPa,122s112s,,7,Desobry-BanonS[3](200MPa240MPa)pH5.05.3,7351.[J].,1995(4):69792GaucheronF.Combinedeffectsoftemperatureandhigh-pressuretreatmentonphysicochemicalcharacteristicsofskimmilk[J].FoodChemistry,1997,59(3):4394473Desogry-BanonS,RichardF,HardyJ.Studyofacidandrennetcoagulationofhighpressuredmilk[J].JDairySci,1994,77:326732744HayakawaI,etal.Denaturationofbovineserumalbumin(BSA)andovalbuminbyhighpressure,heatandchemicals[J].JFoodSci,1992,57:2882925AnenaSG,CreamerLK.EffectsoftheAandBvariantsofboths1-and-caseinmicellesolvationand-caseincontent[J].JDairyRes,1993,60:5055166DumayEM,KalichevskyMT,ChefelJC.High-pressureunfoldingandaggregationof-lactoglobulinandthebaro2protectiveeffectsofsucrose[J].JAgricFoodChem,1994,42:186118687SilvaJL.Pressuredissociationandconformationdriftofthe2-dimertryptophansynthase[J].JBiolChem,1989,264:15863158688HenrikStapelfeldt,etal.Effectofhighhydrostaticpressureonthehydrolysisof-lactoglobulinBbytrypsin,ther2molysinandpepsin[J].JDairyRes,1996,63:1111189NakamuraT,SadoH,SyukunobeY.Productionoflowanti2genicwheyproteinhydrolysatesbyenzymatichydrolysisanddenaturationwithhighpressure[J].Milchwissenschaft,1993,48:14114510.IgG.[C],1998,212311Ferron-Baumy.Coagulationpressuredulaitetdesreten2tatsdultreefiltretion.EffectdeDiversTraitementsTher2miques[J].Lait,1991,71:42343412ShibauchiY.Conformationalchangeofcaseinmicellesbyhighpressuretreament.In:BalnyCeds.HighPressureandBiotechnology[M].INSERM.JohnLibbeeyEurotextLtd.,1992.23924252199910©1995-2003TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.