02-principle病毒学virus巴斯德研究所

PrincipalofViralStructureIPSTetsuyaToyodaFeb.23,2006SizeofvirusEukaryote20mmProkaryote0.2–1mmbacteriarickettsiachlamydiaVirus20–300nmRecenttechnologyformorphologyresolutionElectronmicroscopy50–70A(negativestaining)Cryoelectronmicroscopy10–20ACrystallography3A–NMRScanningtunnelmicroscopyAtomicforcemicroscopy(AFM)ViriongenomeUnitofvirusesMicrobiologyBiochemistry211and0210.5IcosahedralSymmetry20ThreefoldsymmetryIcosahedron60commas,distributedwithicosahedralsymmetryonthesurfaceofasphere.Quasi-equivalentarrangementof180commas,inaT=3icosahedralsurfacelatticeonasphere.20HexagonallatticeT=3latticeParvovirusjelly-rollb-barrelPicornavirusjelly-rollb-barrela-helicesComparisonofthepackingofthejelly-rollb-barreldomainstomatobushystuntvirusNorwalkvirusHIVCapsidModelforassemblyofaT=3virusshell(turnipcrinklevirus)PolyomavirusesAdenovirusadenovirushexonReovirusExpansionofTBSVCa2+TobaccoMosaicVirusRNAviruspackagingsignalsbacteriophageMS2thedual“zincknuckle”HIVTMVPackingofdouble-strandednucleicacidinvirionsBacteriophagelBuddingalphavirusInfluenzavirusendoplasmicreticulumAssemblyandbuddingofretrovirusesHIVMason-PfizermonkeyvirusRSVMuLVSindbisvirus,SemlikiForestvirus,VSVVSVSindbisvirusSemlikiForestvirusVirus–receptorinteractionsPolyomavirusVP1HAsialicacid–a-2,3-Gal-b-1,4-GlcNacInfluenza-virushaemagglutininHAmonomerHA2monomer:thefusion-activeformHAtrimerrearrangedHA2trimerMembranefusionFluCHEF&FluAHAThecorefusionmodules