04-病毒学virus巴斯德研究所

ReplicationofRNAVirusesIPSTetsuyaToyodaMar.4,2006DNARNAProteinDNApolymerasesDNAdepedentRNApolymerasesCentralDogmaDNARNAProteinPrionRNAvirusesViroidsSiRNARNAdependentRNApolymerasePrPscReversetranscriptaseRetrovirusesHBVRibozymeRibozymeRNAWorld:UniqueGeneExpressionStreamofgeneticinformationofDNAviruses＜Group1viruses＞＜Group2viruses＞＜Group3viruses＞＜Group4viruses＞＜Group5viruses＞＜Group7viruses＞dsDNA(+)mRNAdsDNA(+)ssDNA(+)dsRNA(+)ssRNA(+)ssRNA(-)ssRNA(-)ssDNA(-)ssRNA(+)dsDNA(+)mRNAStreamofgeneticinformationofRNAviruses＜Group6viruses＞ssRNA(+)ssRNAmRNAmRNA（±）ByBaltimoreTranscription,Replicationenzymecis-factortrans-factor(cis-element)Transcription:mRNAsynthesistranscriptasepromotertranscriptionfactorterminatorenhancer,silencerReplication:genomereplicaseoriginreplicationfactorcreCascadeofgeneexpressionofRNAvirusesEarlytranscription(negativestrand)LatetranscriptionRNApolymerase(replicase)GenomereplicationStructuralproteins(transcriptase)mRNAgenome(positivestrand)?(CellularRdRp?)InitiationofRNAdependentRNApolymerasevanDijketal.JGenVirol85(2004),1077-1093positive-strandRNAvirusreplicationFerrer-Ortaetal.TheEMBOJournal(2006),1–9ProteinprimingofFMDVpositive-strandRNAvirusreplicationaninternalRNAsequencetermedacis-actingreplicationelement(cre)Yangetal.J.Biol.Chem.,Vol.279,Issue13,12659-12667,2004negative-strandRNAvirusreplicationPan-handlestructurenegativestrandvirusCrowetal.JVirol.2004Jun;78(12):6263-70.Influenzavirus3’5’dsRNAvirusreplicationRetrovirusreplicationHBVlifecycleRTDNAHBVpromoterHBVstem-loopstructureofHBVHBVRT(dimer)retrovirionstructureretroviralRNAgenomereversetranscriptionretrovirusUnintegratedDNAstructuresretrovirusIntegrationoftheviralDNAtoformtheprovirusretrovirusIntegrationoftheviralDNAretrovirusDIgenome3'5'vRNA(-)5'3'DIcRNA(+)DIvRNA(-)3'5'3'5'5'vRNA(-)cRNA(+)cRNA(+)5'3'5'5'5'DIRNA(-)3'5'vRNA(-)3'cRNA(+)5'5'cRNA(+)5'3'5'vRNA(-)cRNA(+)5'ジャンピングポリメラーゼロールオーバー、ループアウトコピーバック図１１　ＤＩゲノムの形成Rollover,LoopoutCopybackPoljumping