木质素的生物降解及其应用

206201012LEATHERSCIENCEANDENGINEERINGVol.20No.6Dec.20101004-7964201006-0027-05*[e-mail]。[e-mail]。、、、、。Q948.12ABiodegradationandApplicationsofLigninLIHai-taoYAOKai*HEQiangJIADong-yingCollegeofLightIndustryandFoodEngineeringSichuanUniversityChengdu610065ChinaAbstractInthispaperthetypesandthedegradationcharacteristicsofligninolyticmicroorganismsandlignin-degradingenzymesareintroduced．Inadditionthepracticalapplicationsofligninbiodegradationinbiochemicalpulpingbiologicalbleachingofpulpbiologicaltreatmentofpapermakingwastewaterfeedindustryandbio-fertilizeraresummarized．Andtech-nologytrendsinligninbiodegradationareoutlook．Keywordsligninbiodegradationmicrobesenzymesapplications1500t。。50%。12。。1—、、1。3。2。。28201Fig．1Chemicalstructureandbondingmodesoflignin2.11Table1Lignin-degradingmicroorganismsandtheirdegradationcharacteristicsPhlebiatremellosusPhlebiasubserialisPhellinuspiniDichomitussqualensAgaricusbisporusStrophariarugosoannulatePenicilliumchrysogenumFusariumoxysporumFusariumsolaniStreptomycescyaneusClostridumxylanoyticumPseudomonasAcinetobacterBacillus－。。1。。、27%。15%10%。4－6。2.2LiP、MnPLacc。CBQ、CDH、GLOXAAO。。。ABTS。、234。2Fig．2SimplifiedreactionofligninoxidizedbyLiP3Fig．3SimplifiedreactionofligninoxidizedbyMnPandLacc629。－15－。、、H2O24。3。。3.1、、。。3.1.1。7。Ceriporiopsissubvermispora27%8。Ceriporiopsissubver-mispora10%～15%10.2%～11.4%9。34%20%72%10。3.1.2、－Laccase-mediatorsystemLMS。22＇－－－3－ABTS、1－HBT、VA、N－－NHA7。－30%11。－1－totallychlorine-free40%85%12。3.1.3、13。5dpH8.91.0COD73%14。L028dCOD84%93%pH615。CoriolusversicolorHydraulicRetentionTimeHRT23h53%～73%16。pH6.0～9.090%17。3.2。。、、。4BacillussubtilisFS、Candidatropicalis、Geotrichcandidum、Lactobacillusbulgaricus29.3%0.37%18。Phanero-chaetechrysosporium10d30200.67%12.51%37.39%85.68%19。、20。3.3。。21。25℃50℃45d7.0%27%6.0%22。50℃35d70%23。Paecilomycesinflatus、14Cβ－6.5%15.5%24。3.4。Phanerochaetechrysosporium25。Ganodermasp．WR-1100mg/L8h96%26。27。Phanerochaetechrysosporium。4、、、。、、、。1LozovikPAKaflyukAE．ApplicationofdifferentialUVspectroscopytothedeterminationofligninsubstancesinPollutedwaterJ．JournalofAnalyticalChemistry2005609833837．2．J．200828359-63．2WangRen-youZengGuang-mingYuHong-yanetal．LignindegradationmechanismbyMicrobesJ．JournalofMicrobiology200828359-63．inchinese3HiguchitT．LigninbiochemistryBiosynthesisandbiodeg-radationJ．WoodscienceandTechnology19902423-63．4SteinbuchelA．Biopolymers-LigninHumicSubstancesandCoalM．BeijingChemicalIndustryPress．20041141-180．5TuomelaMVikmanMHatakkaAetal．BiodegradationoflignininacompostenvironmentareviewJ．Biore-sourceTechnology200072169-183．6．J．2005282104-106．6YuHong-yanZengGuang-mingNiuCheng-gangetal．AdvancesinbiodegradationofligninbyBacteriaJ．En-vironmentalScienceandTechnology2005282104-106．inchinese7．LMSJ．2002171107-112．7ZhanHuai-yuFuShi-yuWuJiao-ping．Rolesofenzyme-catalyzedmediatorsinpulpLMSbiobleachingJ．Trans-actionsofChinaPulpandPaper2002171107-112．inchinese8FerrazACordovaAMMachucaA．WoodbiodegradationandenzymeproductionbyCeriporiopsissubvermisporadur-ingsolid-statefermentationofEucalyptusgrandisJ．En-zymeandMicrobialTechnology20033259-65．9PratimaBajpai．BiochemicalpulpingofbagasseJ．Bio-631technologyProgress2004201270-1272．10ChandraRPLehtonenLKRagauskasAJ．Modificationofhighlignincontentkraftpulpswithlaccasetoimprovepaperstrengthproperties．1．laccasetreatmentinthepres-enceofgallicacidJ．BiotechnologyProgress200420255-261．11．LMSJ．20051074-76．11MinJiang-maFuShi-yuetal．ResearchofLMSbiob-leachinginKraftpulpJ．ChinaPulpandPaper20051074-76．inchinese12CamareroSGarciaOVidalTetal．Efficientbleachingofnon-woodhigh-qualitypaperpulpusinglaccase-media-torsystemJ．EnzymeandMicrobialTechnology2004352/3113-120．13．J．2007262198-203．13JiangLingWuHai-zhenWeiChao-haietal．Researchprogressofcharacterizationofwhite-rotfungusenzymesystemforlignindegradationanditsapplicationJ．Chemicalindustryandengineeringprogress2007262198-203．inchinese14．J．200532876-81．14LiuBoZhangXiao-yuWangHong-xunetal．Mecha-nismdiscussiononAlkalineblackliquordegradedbyAcid-producingWhite-rotfungusJ．EnvironmentalSci-enceandTechnology200532876-81．inchinese15．J．200512088-92．15LiXue-zhiZhaoJianYanFeietal．Bio-TreatmentofwastewaterfromPulpandPaperMillbyWhite-rotfungiJ．TransactionsofChinaPulpandPaper200512088-92．inchinese16DriesselBVChristovL．DecolorizationofbleachplanteffluentbyMucoraleanandwhite-rotfungiinarotatingbiologicalcontactorreactorJ．JournalofBioscienceandBioengineering2001923271-276．17SahooDKGuptaR．Evaluationofligninolyticmicroor-ganismsforefficientdecolorizationofasmallpulpandpa-permilleffluentJ．ProcessBiochemistry2005401573-1578．18．J．2008363214108-14110．18WeiZhi-wenZhaoShi-haoLiBao-ku．StudyonstrawfeedwithmultiplestrainsFermentationJ．JournalofAn-huiagriculturalscience2008363214108-14110．inchinese19PuniyaAKZadrazilFSinghK．InfluenceofgaseousphasesonlignocellulosedegradationbyPhanerochaetechrysosporiumJ．BioresourceTechnology1994472181-183．20．J．200231180-84．20LiangXue-wuYaoLiangLiuQing-huaetal．Effectsofwhite-