percoll分离液的配制

percoll()Percoll()Percoll(20mosmkgHO),1.3gml(2001000g)PercollPercoll()1.()Percoll:9Percoll18.5NaCl1.5MPBS(0.85NaCl0.15MPBS)Percoll()706050403020gml1.0901.0771.0671.0561.0431.0312.Percoll:PercollPercollPercoll3.4.400g2025minPercoll5.Percoll;Percoll,Percoll2()1.NK(LGL):5047.54542.540Percoll,10ml(),Percoll1.21.5mlPBMC110mlNKLGL42.545PercollPercoll2.5030PercollPHA()PBMC,PBMC()Percoll8030Percoll()1.09-1.111.052-1.077B1.062-1.0751.09-1.095T1.065-1.0771.080-1.0851.065-1.0771.050-1.0661.050-1.0701.030-1.060percoll1.1101.1701.03510PBS19percoll1PBS90%70%50%percoll1.5MNaClpercoll190.15MNaCl2*107/Pharmaciapercoll6030percollpercoll15%---75%percoll?80percoll9Percoll18.5NaCl1.5MPBS(0.85NaCl0.15MPBS)Percoll10PBS91100Percoll1.127290mOsmol/kgPercoll()706050403020gml1.0901.0771.0671.0561.0431.031