淋巴母细胞性T细胞淋巴瘤与EB病毒的关系-何妙侠

淋巴母细胞性T细胞淋巴瘤与EB病毒的关系何妙侠　梁国桢　郭瑞珍　邓　飞　肖庆帮　目的:TEB。方法:9TEB,ABC;EBDNA(EBVDNA);RNAEBRNA(EBER1/2)。结果:9T,EBVDNA7(77.8%),EBER1/26(66.7%)。结论:TEB。　T;;;;RNA　R737.33ThestudyofrelationshipbetweenT-lymphoblasticlymphomasandEpstein-BarrvirusHeMiaoxia,LiangGuozhen,GuoRuizhen,etal(DeptofPathol,ZunyiMedicalCollege,Zunyi　563003)ABSTRACT　Purpose　ToexploretherelationshipbetweenT-lymphoblasticlymphoma(T-LBL)andEp-stein-Barrvirus(EBV).Methods　NinecasesofT-LBLweredetectedforEBV.ThenatureofneoplasticcellswasconfirmedbyimmunohistochemistryEBVspecificDNA(EBVDAN)andEBVencodedRNA(E-BER1/2)weredetectedwithpolymerasechainreaction(PCR)andRNAinsituhybridization(ISH-RNA).Results　SevencaseswereEBVDNA-positive(77.8%)andsixcaseswereEBER1/2-positive(66.7%).Conclusion　ItindicatesthatEBVisassociatedwithT-LBL.KEYWORDS　T-lymphoblasticlymphoma;immunohistochemistry;polymerasechainreaction;RNAinsituhybridization:,　563003:,,28,,。:　　T(T-LBL)T(T),Sternberg,,T〔1〕。EB(EBV),、Burkitt、T,T-LBLEBV,〔2～5〕。EBVEB,T-LBLEB。1　9T-LBL1991～1996。10%,,HE。1.1　　DakoUCHL1、L26MT-1。ABC。1.2　(PCR)　(1)DNA:5～7μm6～8Eppendorf,,200μl(10mmol·L-1Tris-HClpH8.0;Tween0.5%;K200mg·L-1)。56℃3h,95℃10min。(2):EBVMishima〔6〕,,DNA123bp。(3)PCR:25μl(50mmol·L-1KCl,10mmol·L-1Tris-HClpH8.0,1.5mmol·L-1MgCl2,0.01%,dATP、dGTP、dCTP、dTTP20mmol·L-1,0.5μl,Taq(Gi-bo)1U,25ngDNA)。99℃5min,95℃5min,61℃90s,72℃2min,90℃90s,61℃30s,72℃90s38,72℃10min,4℃,Perkin-ElmerGetus480,10μlPCR8%,,,EBV·256·临床与实验病理学杂志　JClinExpPathol　1998Jun;14(3)DOI:10.13315/j.cnki.cjcep.1998.03.026,DNA。1.3　RNA(RNA-ISH)　(1):EBV30Mer(GBI),EBVRNA(EBER1/2)。(2):4～6μm3-(APES),1,,DEPC5min,0.2%mol·L-1HCl(5min)5g·L-1(37℃,15min),54℃10min,37℃,TBS,3%H2O25min,-(S-P),37℃30min,DAB(),。EBV,。2　2.1　　9T-LBL,8,1,8∶1,7～65,36,5。1,6,1,1。,。,。,6～9μm,。,。,,,(1);。7,2。,,,,,5～10/HPF,,。,3“”。。,,,,。2.2　　9T-LBLUCHL1,MT-1,L26,L26B。2.3　　9T-LBL7EBVDNA(77.8%),123bp,1。2.4　　9T-LBL6E-BER1/2(66.7%),,,(2)。EBER1/2,150%(3),5(4)。,,HE,E-BER1/2。3　EBVT,,TEBVB〔7〕。9T-LBLEBV,EBVDNA77.8%,EBER1/266.7%,EBVT-LBL,〔5〕TEBV62%。,BC3dEBV,B,EBV。Watry〔8〕EBV,,EBV,,EBV,,EBVT。EBVEBV,T-LBLT(T),,EBVTT-LBL。,T,EBV,,EBVT,TEBV(C3d)EBV〔9〕。,EBVT,EBVC3dT,T。,,T-LBLEBVTEBV,。PCREBV,,RNA、、,,EBV,T-LBLEBV。RNA-ISH,E-BER1/2。150%,55%,E-BER1/2,T。·257·临床与实验病理学杂志　JClinExpPathol　1998Jun;14(3),T-LBLEBV,EBV,〔5〕,T-LBLEBV,,。T-LBLEBV,EBV。参考文献1　.淋巴结病理学.:,1989:2672　SuIJ,HsiehHC,LinKH,etal.AggressiveperipheralT-celllymphomscontainingEpstein-BarrviralDNA:aclinicopatho-logicandmolecularanalysis.Blood,1991;77:799～8083　OttG,OttMM,FellerAC,etal.PrevalenceofEpstein-BarrvirusDNAindifferentTcelllymphomaenitiesinEuropeanpop-ulation.IntJCancer,1992;51:562～5674　BruinPCD.DetectionofEpstein-Barrvirusnucleicacidse-quencesandproteininnodalTcelllymphomas:relationbetweenlatentmembraneprotein-1positivityandclinicalcourse.Histopathology,1993;23:509～5185　ZhouXG,Hamilton-DutoitSJ,YanQH,etal.HighfrequencyofEpstein-BarrvirusinChineseperipheralTcelllymphoma.Histopathol,1994;24:115～1226　MishimaK,HoriuchiK,KojyaS,etal.Epstein-Barrvirusinpatientswithpolymorphicreticulosis(lethalmidlinegranuloma)fromChinaandJapan.Cancer,1994;73:3041～30467　Hamilton-DutoitS,PallesenG.ASurveyofEpstein-Barrvirusgenesexpressioninsporadicnon-Hodgkinslyphomas:detectionofEpstein-BarrvirusinasubsetofperpipheralTcelllym-phomas.AmJPathol,1992;140:1315～13258　WatryD,HedickJA,SiervoS,etal.Infectionofhumanthymo-cytesbyEpstein-Barrvirus.JExpMed,1991;173:971～9809　RichelDJ,LepoutreJM,KapsenbergJG,etal.Epstein-BarrvirusinaCD8-positiveTcelllymphoma.AmJPathol,1990;136:1093～1099(1997-07-24,1997-10-24)(图片见插页第34页)、　　,、、、。,、,,、。。:()、()、()、()、()、()、()、()、()。1　　、。、、。。:19987302　　、、3000,3001。、、500～800。《》19981,,。3　　1998111～8,111,2～7,8。:,。,《》(),,。。:350002　568。:　。:0591-3720685　3732741　:191/192-3884568　,。·258·临床与实验病理学杂志　JClinExpPathol　1998Jun;14(3)