白血病免疫分型

免疫学标志分析在白血病中的应用血液免疫科葛繁梅一、免疫学分析的优点及局限性提高恶性血液病诊断与分型的准确性,检测残留病灶，判断预后区分淋巴细胞与髓性，区分T细胞与B细胞确定白血病细胞的阶段覆盖率高于基因及染色体，尤其对淋巴系统敏感性高于形态及染色体比染色体及基因分析快捷，但特异性比之差解释结果的医师要有丰富的知识对M4、M5、M6、M7的鉴别不太敏感二、骨髓细胞在流式细胞参数图中的定位三、Abnomalcell四、正常血细胞发育过程中抗原的表达五、白血病具体类型分析R2R3R4AML-M1File:wangqingming.007AcquisitionDate:22-Nov-04Region%GatedR1100.00R20.99R394.63R40.25R2R3R4灧瑎撓崳浪湹碧栒埃R3捄94.63%,碧ピCD34,HLA-DR,CD13,CD38,cMPO,婙忳碧ピCD117,CD33.寵撓崳浪湹敵鲄湊佫埃罕枒浪枒伋棛姲尌忳栒．AML-M1)鴧儖枒ホ埃AML-M1File:baixiurong.001AcquisitionDate:22-Apr-05Region%GatedR1100.00R232.40R324.90R423.91R513.59R61.86R2R3R4R5R6AML-M2File:wangxing.001AcquisitionDate:05-Jan-05Region%GatedR1100.00R28.91R376.38R41.06R512.67R2R3R4R5AML-M3R2R3R4R5R6AML-M4File:wangyushu.001AcquisitionDate:13-Dec-04Region%GatedR1100.00R214.67R310.11R417.55R536.03R64.20R73.24R2R3R4R5R6R7灧瑎撓崳浪湹碧栒R3捄26.55%,碧ピCD34,CD117,HLA-DR,CD38,CD33dim婙彌湊佫碧ピCD64,CD13,寵撓崳浪湹敵鲄湊佫埃R％捄36.88%,碧ピCD64,CD38,HLA-DR,CD14,CD33,CD13寵泊崊犗禃澙湊佫埃R7捄8.1%,SSC灉ホ（碧ピCD33,CD13寵牷婪枒。湊佫埃罕枒浪湹湊佫伋棛姲M4E0鴧儖枒ホ埃AML-M4E0File:huguangqing.001AcquisitionDate:16-Jun-05Region%GatedR1100.00R24.08R365.72R417.70R55.36R63.47R2R3R4R5R6AML-M5File:fangchanghua.004AcquisitionDate:08-Nov-05Gate:G1Region%GatedXMeanYMeanR1100.0074.5593.45R29.9243.53621.59R370.0845.896.55R47.2055.5969.99R53.20236.75268.43R65.49329.4183.33R2R3R4R5R6撓崳浪湹碧栒埃R4捄7.2%．彨澫湹湊佫24%),碧ピCD34,CD117,CD13,CD33,HLA-DR,姫碧ピCD22,CD19,cCD3,MPO,GlyA,CD56,CD7,寵浪湹敵鲄湊佫埃R3捄70.08%（碧ピGlyA,寵敄澙澫湊佫埃罕枒澫伋棛姲(AML-M6)鴧儖枒ホ埃AML-M6File:chenjianhui.001AcquisitionDate:17-Feb-05Region%GatedR1100.00R217.81R328.50R436.75R53.08R67.66R2R3R4R5R6B-ALLFile:baiyanyu.001AcquisitionDate:03-Mar-05Region%GatedR1100.00R27.02R375.60R411.13R2R3R4B-ALLFile:sunhongwei.001AcquisitionDate:08-Dec-05Gate:G1Region%GatedXMeanYMeanR1100.00130.88118.47R26.5368.18492.38R378.1486.4088.92R49.64376.6090.53R51.67208.22393.93R2R3R4R5撓崳湹碧栒埃R3捄78.14%,碧ピCD34,CD22,CD19,CD10,cCD79a,CD20（婙忳湊佫碧ピCD13.姫碧ピCD33,CD117,cCD3,MPO,寵撓崳B湊佫（鬟嶗罕枒B佌湊佫伋棛姲．ALL-B）捲帨埃B-ALLFile:gaoyanlai.001AcquisitionDate:15-Jul-05Gate:G1Region%GatedXMeanR1100.00378.54R23.9074.38R326.9371.22R464.34532.80R51.82254.28R61.5257.10R79.4178.04R81.6459.98R2R3R4R5R6T-ALLFile:yangxingxin.003AcquisitionDate:23-Nov-05Gate:G1Region%GatedXMeanYMeanR1100.0035.17297.72R242.3831.17324.59R346.5432.76151.63R48.6752.23893.72R2R3R4撓崳湹碧栒埃R3捄46.54%（碧ピCD34,cCD3,CD5,CD7,TdT,恍碧ピCD3,姫碧ピCD4,CD8,CD117,CD13,CD22,MPO,cCD79a,CD19,CD16,CD56,CD25,CD2,寵敵鲄T湊佫埃R2捄42.38%（碧ピcCD3,CD25,CD5,CD7,恍碧ピCD3,姫碧ピCD4,CD8,CD117,CD13,CD34,TdT,CD22,MPO,cCD79a,CD19,CD16,CD56,CD2,寵敵鲄T湊佫埃罕枒T佌湊佫伋棛姲(ALL-T)鴧儖枒ホ埃T-ALLHAL