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1.2.23.4.5.6.98/2k/xp/NT(021)64951899-232shanghai01051665161-222Pekin02085524840-1029Guangzhou11.1NanoDrop®ND-1000220nnm750nm1ulNanoDrop®ND-1000751.21ul1umCCD1.3UV/VISNanoDrop®ND-10001ulNanoDrop®ND-1000l3700ng/uldsDNAllA280100mg/mlBSAlBradfordlBCAll/22.12.1.1NanoDroplMicrosoftWindows98win2000WinXpWin95WinNTl233MHZlCDROMl32MBl40MBlUSBUSBlMicrosoftExcel2.1.2USB1.USB2.“install”3.USB4.“ok”WindowsXPWindows2K”Thesoftwareyouareinstallingforthishardware:ceanOpticsUSB2000-EEPROMloadhasnotpassedWindowslogotesting….””continueanyway”5.“”2.1.3NanoDrop“MSSansfont8point”1.“”2.“”“”“”3.“”“MicrosoftSansSerif”“8point”4.“”2.1.4NanoDrop:\nanodropdate“Archiving”“Duplicatedatestorage”“DuplicateDateFolder”“SavePreferences”3.4.2“AutoReporting”“log”“log”“Passwords.log”3.5“Troubleshooting”3.63.6.1Level101010“Administrator”“nanodrop”10Level55C:\nanodropdateDefaultlevel0C:\nanodropdate3.6.2ll99l103.6.33.6.4Password.logIDc:\nanodropdata\log“password.Log”“userpreferences.Log”c:\NanoDropData\LogFilesc:\programfiles\NanoDrop{version}c:\programfiles\NanoDropV3.3.03.7//1.2.260nm260nm‘OK’,“InitializingSpectrometer-pleasewait”4.2EscapeESCEscapeEscape4.3MeasureF1MeasureMeasure4.4BlankF34.512“Black”F3344.6ReblackF2ReblackBlackReblackReblack4.7F44.8“File”4.9JPG“File”JPG4.10/F6“”“Recording”“StartReport”&&c:\NanoDropDatasection3“”4.11F5“PrintReport”F54.12F7“ShowReport”4.13SampleID5.NanodropND-1000“NucleicAcid”5.11-2ul5.25.3SampleType“DNA-50”DNARNA-40RNAssDNA33DNA“other”DNA-50‘other’15150?andAbs/A260260nm10mm1mm100.2mm50A280280nm10mm1mm100.2mm50260/280260280DNARNADNA1.8RNA2.0280260/230260280260/230260/2801.8-2.2ng/ul260nmShowReport2005.4340nm005.56MicroArrayND-10000.2pmol/ml6.1MicroArray9‘Dye/ChromophoreEditor’Dye1Dye2260nm280nmNanoDropDye1Cy3Dye2Cy5‘None’‘None’260nm280nm0“DNA-50”DNARNA-40RNAssDNA33DNA“other”DNA-50‘other’15150?andAbs:/Dye1(or2):Abs.Norm:1mmpmol/ul:ng/ul260nm340nmShowReport200260/230260230260/230260/2801.8-2.26.5&750nm400750nmDye1Dye27.UV-VISUV-VISND-1000220nm750nm7.11ul1.5-2ul7.2ND-100010mm15AND-10005000.2mm10mm75A7.3?1/Abs1and?2/Abs21mmup/downHiAbs10mm75A“HiAbs”0.2mm0.1mmID“C:\NanoDropData\Username\HiAbs”ExcelNormalize400700nm2008.A280A280280nm280nm0.2mmA28010mm8.12ul8.215-208.38.4SampleType:6’SampleType’0.11mg/ml280nm1.0A10mm0.110mg/mlBSA280nm6.7IgG0.110mg/mlIgG280nm13.70.110mg/ml280nm26.4M-1cm-1MWe999X1000MW9999X1000?andAbs:/A28010-mmPath:10mm260/280260280200340nm00YY‘Blank’9.&A280nmMicroArray9‘Dye/ChromophoreEditor’Dye1Dye2260nm280nmNanoDropDye1Cy3Dye2Cy5‘None’‘None’260nm280nm0:NormAbs@?3:10mmDye1(or2):NormAbs@?1:10mmuMAbsratio?1/?3:1?3mg/ml280nm340nm200750nm400-750nm750nm10BCABCA(BicinchoninicAcid)280nmA280nmBCA562nmCuBCA750nmBCACuSO42ul15-20BCAKitsProtocols,andSamplePreparationBCAKit/20/14ul80ulBCA/1/110ul10ulBCAPCR60BCABSAprotocolND-1000ViewStandardCurve(F8):SampleType:ReferenceStandards1-7,andSamplereferenceReplicate#:referenceResetWindow(F11):ResetThisStd(F12):Absorbanceat562nm:CuBCA1mm562nm?andAbs:/mg/ml:200BCABCA7531BCA253‘StandardCurve’‘ViewStandardCurve’TheDeletePoint‘DeletePoint’‘Resetthisstd’‘ResetThisWindow’(F11)BCA11LowryLowryKitsProtocolsLowry/5120ul100ullowry1010ulFolin–Ciocalteu30BCA12BradfordBradfoldKitsProtocols/5014ul200ulBradford/1110ul10ulBradfordPCRBCA13ND-1000ND-10001mmvs1cm10250700nm600nm2ul600nmAbsorbance:?1600nm1mm?andAbsorb.:Baseline:MaxAbsorbance:20014Excel“NucleicAcid20051107.ndj”2005117.ndj1A2801.0cmUV-VisBCABradfordLowry1.0mmUV-Vis0.1mm2‘’‘’‘’‘Reblank’‘Blank’‘Reblank’C:\NanoDropDataUsernameApplicationModule(BCAProtein,Lowry,Bradford,CellCulture,MicroArray,NucleicAcid,ProteinA-280,Proteins&Labels,UV-VisorUV-VisHiAbs)ArchivingTab‘Duplicatedatastoragebox’On‘SavePreferences’NanoDropNanoDropND-100023,tabs3Configuration‘AutoScale’,‘Includegraphinprintout’‘Includestandardsinprintout’.DataReportFilePageSet-upPrintWindow:‘CTL+P’SaveWindow:.JPGsHelp/ContextHelp:Import“Data”‘ImportSample’Importfolder:Directorytree:HiAbsExcelArchiveFileConverter:NanodropND-1000c:\nanodropdata3.2.ndjor:200SearchIDSampleInformationandSpectrum:ImportandReturnshiftcontrolPlotsPlotsMethod:Date:SelectedPlot:‘SelectPlot’Plots/Sets:2020Legend:Sampleinformation:Movablexandyaxis:Reports‘ConfigureReport’‘OK’Sort:Savereportformat:ndf‘UsersPreferences’‘Report’“SelectDefaultReportFormat’LoadReportFormat:Printreport:configurationSaveReportandLoadReport:LoadReportUserPreferences.ndvExcelC:\NanoDropData\ReportsMethod:Dateandtime:ReportName:Buffermode:MaxBuffersize:200‘’‘’c:\Na