生物样品前处理的研究进展-冯健男

··冯健男，杜守颖*，白洁*，陆洋，刘慧敏(北京中医药大学中药学院，北京100102)、、、、。、、、、、。2014-07-10“”2014ZX093013060092011-CXTD-132014-QNTSZX009*[e-mail]*[e-mail][e-mail]。、、、。、。、。、。。、、、。、ng～pg。、1。。。。1。。。0.9%1r·min－110min2。1～3、、。。＞＞＞-3。6%pH。。424～150000μg·L－195.99%。、、。5。。6。。·3414·3921201411Vol.39Issue21November2014。pH。、。。。、、。2---、-、。-LLE。-、、。AＲ、、。。、、、7。80.3mL0.06moL·L－1HCl。80%。TaberneroMJ9250.1%0.01%4h。-0.5～25μg·L－10.1μg·L－1。-、pHbackextractionpH。-、pHcounterion10。Ｒ4N+CH2Cl2。-。。11。3solidphaseextractionSPEHPLCGC。SPE4SPE、、、12。、、。、。。。。3.1、、。-1∶5～1∶10-30～40。、。。、13。3。。。。。。3.2。pH14pH。3.3。。。。。·4414·3921201411Vol.39Issue21November2014Horspool157、、、、、72%～89%。、、、、、16。。。4molecularimprintedpolymerMIP17。templatemolecularfuncitionalmonomercrosslinker。MIP。-“”。、、、。MIP181。1Fig.1Preparationofmolecularlyimprintedpolymer、。MIP--。。19。“”。202MIPsMIPs、、。21。5SPME2080。SPME2Di-SPMEHs-SPME。、。polydimethylsiloxanePDMSPDMS/divinylben-zeneDVB、carbowaxCAＲ/PDMScarbonmolecularsieveCW/DVB、CW/-。。、。-、-。。invivoSPMEmulti-wellplatetechnologyhigh-throughputSPME22。SPME。SPME。SPME-。SPME23。24-、13。10～500μg·L－1＜10μg·L－1ＲSD＜10%。6206060、25-27、28。microdialysis·5414·3921201411Vol.39Issue21November2014、、、、。。SPELLEMD、、。。。、、、、、、、。、、、、、29-30。、、HPLC、CE、LC-MS。Ｒong-HuaMa31LC-MSA、、。32、CA1HPLC。82.08%。MarinaMCar-rozzo33LC-MS/MS、。7MSPEMSPE。。。。、、34。FeCoNi2。、、。。、。35Fe3O4MNPsMNPs/PSt2Fe3O4MNPs66.81emu/gMNPs/PSt38.82emu/g。MSPE。、、、36。8。、37-39。2。。。、、、。、、。98、、、SFE、、、。。、、、。、。1.J.2014430.2.D.2008.3MacekJKlimaJPtáekP.Ｒapiddeterminationofvalsartaninhumanplasmabyproteinprecipitationandhigh-performanceliq-uidchromatographyJ.JChromatogrB20068321169.4.C.2009.5.J.200831183.·6414·3921201411Vol.39Issue21November20146.D.2008.7.J.2013336484.8.16^#J.20062751.9TaberneroMJFelliMLBermejoAMetal.DeterminationofketamineandamphetaminesinhairbyLC/MS/MSJ.AnalBio-analChem20093592547.10.J.2000318.11.C.“”2013.12.-D.2013.13.J.201032439.14.J.201331290.15HorspoolLJIMcKellarQA.Determinationofshort-chainfattyacidsinequinecaecalliquorbyionexchangehighperformanceliquidchromatographyaftersolidphaseextractionJ.BiomedChromatogr199155202.16VuckovicD.Currenttrendsandchallengesinsamplepreparationforglobalmetabolomicsusingliquidchromatography-massspec-trometryJ.AnalBioanalChem201240361523.17.J.200671035．18.D.2013.19.D.2008.20.MIPsJ.200623435321.J.2013595119.22DajanaVuckovicSanjaＲisticevicJanuszPawliszyn.Invivosol-id-phasemicroextractioninmetabolomicsopportunitiesforthedi-rectinvestigationofbiologicalsystemsJ.2011505618.23LordHLZhangXMusteataFMetal.Invivosolid-phasemi-croextractionformonitoringintravenousconcentrationsofdrugsandmetabolitesJ.NatProto201166896.24.--J.20096729.25.-J.201129293.26SyslováKＲambousekLKuzmaMetal.Monitoringofdopa-mineanditsmetabolitesinbrainmicrodialysatesmethodcombi-ningfreeze-dryingwithliquidchromatography-tandemmassspec-trometryJ.JChromatogrA20111218213382.27NirogiＲAjjalaDＲKandikereVetal.LC-MS/MSmethodforthequantificationofalmotriptanindialysatesapplicationtoratbrainandbloodmicrodialysisstudyJ.JPharmBiomedAnal201381160.28GuoPWangXLiuLetal.Determinationofmethotrexateanditsmajormetabolite7-hydroxymethotrexateinmouseplasmaandbraintissuebyliquidchromatography-tandemmassspectrometryJ.JPharmaBiomedAnal20074351789.29.J.201319244506．30．J．20121812303.31MaＲong-huaYangJieQiLian-wenetal.Ｒong-HuaMainvi-vomicrodialysiswithLC-MSforanalysisofspinosinanditsinter-actionwithcyclosporinAinratbrainbloodandbileJ.JPharmBiomedAnal20126122.32.-J.2012176654.33CarrozzoMMTroisiLCannazzaGetal.AnimprovedLC-MS/MSmethodforthequantitationofadenosineconcentrationinmicebrainmicrodialysatesJ.JPharmBiomedAnal201270563.34.J.20123010979.35.D.2013.36.D.2012.37JeongJYLeeJHKimEYetal.Determinationofphthalatemetabolitesinhumanserumandurineasbiomarkersforphthalateexposureusingcolumn-switchingLC-MS/MSJ.SafHealthWork20112157.38BeheraDPattemＲKumarMSetal.Utilityofacolumn-switc-hingLC/MS/MSmethodincytochromeP450inhibitionassaysusinghumanlivermicrosomesJ.DrugMetabolDrugInteract2013283177.39ZhengQWangFLiHetal.QuantitativeanalysisofolanzapineinratbrainmicrodialysatesbyHPLC-MS/MScoupledwithcol-umn-switchingtechniqueJ.JChromatogrBAnalTechnolBi-omedLifeSci2012905127.·7414·3921201411Vol.39Issue21November2014ＲesearchprogressofpretreatmentofbiologicalsamplesFENGJian-nanDUShou-ying*BAIJie*LUYangLIUHui-minSchoolofChinesePharmacyBeijingUniversityofChineseMedicineBeijing100102ChinaAbstractSuitablepretreatmentofbiologicalsamplescantrulyreflecttheroleoflawofthemeasuredcomponentsplayedinthebodyandwillprovideexperimentalevidenceforthestudiesonmetabolicprocessmaterialbasisofefficacymechanismofactionpharmacologytoxicologyandtheothers.Biologicalsamplesincludebloodurinehairtearsetc.Therearealsomanysamplespro-cessingmethodssuchasthedirectproteinprecipitationliquid-liquidextractionandsolidphaseextractionandsoon.Thesemethodscouldbeusedaloneorcombined.Keywordspretreatmentofbiologicalsamplesadvancesinresearchmethodsprospectsforresearchmethodsdoi10．4268/cjcmm20142112·8414·3921201411Vol.39Issue21November2014