ELISA缓冲液配制1.包被缓冲液:0.05mol/L、PH9.6碳酸盐缓冲液试剂剂量无水Na2CO31.59gNaHCO32.93gDH2O1000mlpH9.6,4℃保存。2.洗涤液:0.01mol/L、PH7.4PBS-Tween20试剂剂量NaCl8gKH2PO40.5gNa2HPO42.9gTween200.5mlDH2O加至1000ml3.底物溶液(OPD):PH5.0磷酸-柠檬酸缓冲液试剂剂量0.2mol/LNa2HPO425.7ml0.1mol/L柠檬酸24.3mlDH2O50mlOPD4mg30%H2O20.015ml4.终止液:2mol/LH2SO4