大孔吸附树脂在中草药研究中的应用进展-米靖宇

[]米靖宇,宋纯清(上海中医药大学,上海200032):;;:,:R932:B:1001-1528(2000)12-0914-04AdvancesofApplicationofMacroporousResininStudyofTraditionalChineseHerbsMIJing-yu,SONGChun-qing(ShanghaiUniversityofTraditionalChineseMedicine,Shanghai200032,China)Keywords:macroporousresin;traditionalChineseherbs;separationandenrichmentAbstract:TousemacroporousresininstudyofeffectivecomponentsoftraditionalChinesemedicinemoreeff-iciently.AnreviewwascomposedofcharacterofmacroporousresinanditsapplicationintraditionalChineseherbs.:2001-01-20:(1974~),,,,::(021)5423171070,,,,,1[1,2],20~60,,,,,,,2[1]2.1树脂本身化学结构的影响,,,2.2溶剂的影响,,,9142001122312中成药ChineseTraditionalPatentMedicineDec.,2001Vol.23No.12,,,DDA-,,,2.3被吸附的化合物的结构的影响,,,,3[2],,,,,3.1上样溶液的pH值2.23.2树脂柱的清洗,,,3.3洗脱液的选择,,,,4,[3]:D-101DA-201MD-05271GDX-105CAD-40XAD-4SIPD-G[4]4.1在皂苷类化合物分离富集中的应用D-101,,,94.58%,,[5]Rg1,YPR-Ò,70%Rg1,Rg1,Rg1,Rg1[6],,101.38%,[7]D-101,,800mL/L,[8]D-101,,99.79%,[9]D-DA-2014.2在黄酮类化合物分离富集中的应用,70%,,70%TLC9.92%(84.58%),5.42%,2,,,[10]39152001122312中成药ChineseTraditionalPatentMedicineDec.,2001Vol.23No.12,,,,[11],AB-8,,[12]4.3在苷类化合物分离富集中的应用,GDX-104,,,,30%,98.9%,400,[13]70%,20%,,,5%,75%[14]DA-201,1.5%,[15],AmberliteXDA-4,6.14%,;D-101,8%,3%,;D-,,4.4在酚性和酸性化合物的分离富集中的应用,D-101,20%,,[16],,D-101,,[17],,,30%,,25%~29%,0.6%,[18]D-101,98%,86%[19](),,99.42%,[20]4.5在生物碱类化合物分离富集中的应用[21],,AB-8,50%,,,4.6在其他类化合物分离富集中的应用SOD,SIP-1300,-,9.8%,11.3[22],,CDA-40pH5~64g/100mL70%4h,85%,93%,[23],[24],LD605,:;,,,59162001122312中成药ChineseTraditionalPatentMedicineDec.,2001Vol.23No.12,,,,[21]:ADSADS-17,,-25%~40%,6.5%~10%,:\24%,\6%(-20%)ADS-7,,AB-8HP-20,,-90%,98%,,,(),,,,::[1].[J].,1980,11(3):138-141.[2],,.[J].,1990,21(8):42-44.[3].-[J].,1984,(3):47-48.[4].[J].,19(12):40-41.[5],,,.[J].,1998,21(3):165-166.[6],,,.Rg1[J].,1996,16(2):112-113.[7],,,.[J].,1995,26(5):282-285.[8],,,.[J].,1999,22(1):29-31.[9],,.[J].,1995,10(4):150-151.[10],,.[J].,1999,14(4):154-155.[11],,,.[J].,1996,27(1):80-81.[12],,,.[J].,1998,23(12):735-736.[13],.-[J].,1997,28(8):462-463.[14],.[J].,1998,29(10):664-667.[15],,.[J].,1994,19(1):31.[16],,,.[J].,1998,14(3):52.[17],,.[J].,1998,10(3):135-136.[18],.[J].,1999,24(4):215-217.[19],,,.[J].,1999,15(2):86-87.[20],,.[J].,1996,21(8):484-485.[21],,,.[J].,2000,35():99-100.[22],,.SOD[J].,1995,26(1):1-2.[23],,.CDA-40[J].,2000,22(3):187-190.[24],,,.[J].,1998,23(10):606-609.9172001122312中成药ChineseTraditionalPatentMedicineDec.,2001Vol.23No.12