纤维素酶活力测定方法

,2010,14(4):151-153JournalofHebeiAgriculturalSciences康纪婷1,吴翔2,甘炳成2,张小平1*(1,625014;2,610000):关于纤维素酶活力测定方法很多,至今也没有统一国内许多单位根据各自的实验目的采用了不同的测定方法,并据各自的经验对其做了部分修改,使得测定方法更加多样化综述了纤维素酶活力的测定方法及其存在的问题,旨为不同目的纤维素酶活力测定方法的选择提供借鉴:纤维素酶;酶活力;测定方法:TQ9201:A:10081631(2010)04015103DeterminationMethodsofCellulaseActivityKANGJiting1,WUXiang2,GANBingcheng2,ZHANGXiaoping1*(1CollegeofResources&Environment,SichuanAgriculturalUniversity,Yaan625014,China;2MicrobiologyLaboratoryofSoilandFertilizerInstitute,SichuanAcademyofAgriculturalSciences,Chengdu610000,China)Abstract:Thereweremanydeterminationmethodsofcellulasactivity,whichwerenounifiednowDifferentdeterminationmethodswereusedbasedondifferentexpermientpurposesinmanyinstitutionsinChina,andsomeofthemweremodifiedaccordingtoexperienceThedeterminationmethodsofcelluloseactivityandproblemsweresummarized,whichwouldprovidereferencesfortheselectionofmethodsbasedondifferentexpermientpurposesKeywords:Cellulase;Activity;Determinationmethod:20100324:(2008VJJ018):(1984-),,,,Emai:[e-mail]:111,,[1]D14,1,,,,,,,,,,1,[2],12(Cellulase)1,C1CX2,3[3,4],,3[5]121内切葡聚糖苷酶CxCMCendo14Dglucanase(EG,Cne)Cx,14,,122外切葡聚糖苷酶C1exo14Dglucanase(CBH,Cex)C1,14D,1,(cellobiohydrolase)123葡萄糖苷酶14glucosidase(BG),,,,221[8],:2010211棉线切断法,,pH,212滤纸崩溃法,,,pH,,pH,(2),,,,213羧甲基纤维素钠为底物的粘度减少和还原糖增加的测定法[9]CMC,3min18min,,1/2,l0min1/21,214分光光度计法[10],500nm,,,,,,215快速测定纤维素酶CMC液化活力法[11],,,,216平板法,,,,217巴鲁阿斯温(Barnchandswain)测定法,D,22,DNS,35(DNS),,[12]221内切纤维素酶活(CMCaseactivity),CMCAmano,[14],Merz[15]222外切纤维素酶活(PNPCase)(pNPC),[15](Avicel),AvicelaseAvicel,,;,223纤维二糖酶活,,,,(PNPG),224滤纸酶活1984,,,,,,225差重法,,,,,,[16]331,,,[6]:(1),,,,,,(2),,,,(3),,,1524:,,[7]32,,,,,,,,,,,,,,33,,,,1,1,1:[1],,,[J].,2009,37(1):11-12,15[2],,箎纤维素酶测定方法的研究[J].,2000,23(2):45-48[3]BerndNidetzky,WalterZachariae,GntherGercken,etalHydrolysisofcellooligosaccharidesbyTresseicellobiohydrolases:Experimentaldataandkineticmodeling[J].EnzymeMicrobTechnol1994,16(2):43[4],,,[J].,2008,36(24):10303-10304[5],[J].,2004,21(4):72-76[6][J].,1994,24(4):27-32[7],,[M].:,1997[8],,,[J].,2006,(6):6-9[9][J].,2002,(8):38-39[10]B()()[M].:,1992:84-112[11]CMC[J].,1979,(5):11[12],[J].,2006,(11):67-69[13]WoodTMThecellulaseoffusariumsolanipurificationandspecificityofthe(14)glucanaseandtheDglucosidasecomponents[J].BiochemJ,1971,121:353[14]ChristalopousP(1994)PurificationandcharacterizationofanextracellularglucosidasewithtransglycosylationandexoglucosidaseactivitiesfromFusariumoxysporum[J].EurJBiochem,1994,224:378-385[15],,[J].,2007,44(5):663-666(150)[9],[J].,1999,21(12):644-645[10],[J].,2009,37(17):7981-7982[11],,,[J].,2006,34(13):3176-3177[12],,,[J].,1997,13(6):59[13],,[J].,2004,32(6):25-28[14],,,AB8[J].,2006,22(5):309-3l1[15],,,[J].,2004,35(4):361[16]PpeT,RenDM,ShiJStudyonpurificationandpropertiesofantibacterialsubstancefromantagonisticbacteriaP72againstwheatscab[J].AgriculturalScience&Technology,2008,9(6):124-126[17]ZhangYF,HanCH,LinJ,etalExpressionofporcinereproductiveandrespiratorysyndromevirusORF7geneandpurificationandimmunologicalactivityanalysisoftherecombinantprotein[J].AgriculturalScience&Technology,2009,10(2):62-67,72[18]ChenY,TianH,YinSH,etalPurificationandimmunocompetenceanalysisofGP5proteininproteinreproductiveandrespiratorysyndrome[J].AgriculturalScience&Technology,2009,10(3):97-100153