甲基汞检测研究进展

223220112ChemicalResearchandApplicationVol．23No．2Feb．20111004-1656201102-0129-08*1160122010-07-062010-10-282107603220070141052DUT10LK051976-。[e-mail]。。、GC-MS、GC-ICP-MSHPLC-ICP-MS、。、、。。O657AProgressindetectionofmethylmercuryWANGSongLIHong-linHUMing-mingPENGXiao-junFANJiang-li*StateKeyLaboratoryofFineChemicalsDalianUniversityofTechnologyDalian116012ChinaAbstractMercuryisacommonpollutantintheenvironmentunderthemethylationofmicrobeitcanbeturnedintomethylmercurywhichisoneofthemosttoxicformsofmercurycausingirreversibledamagetothecentralnervoussystemthroughtheaquaticfoodchain.Theseproblemshavepromptedthedevelopmentofmethodsforthedetectionandquantificationofmethylmercury.Recentlysensitiveandselectivemethodsformethylmercuryanalysishavebeendevelopedpropellingaremarkablegrowthindetectionofmethylmercury.SpectrometrychromatographyespeciallyhyphenatedinstrumentaltechniquessuchasGC-MS、GC-ICP-MSandHPLC-ICP-MShavebeenexploitedtodetectandquantifymethylmercury.Despitethegreatadvancesmadeinmethylmercuryanalysisthepaceofresearchremainslimitedbythelengthytimeandhighcostofmethylmercuryanalysisusingcurrentmethods.Becauseofthehighsensitivityandselectivityquickresponsetimeandeasysignaldetectionchemicalsensortechniqueshavebeendevelopedremarkablyinthefieldofelementanalysis.Thispaperhassummarizedthemajorinfluencesofmethylmercuryconventionalinstrumentaltechniquesandchemicalsensortechniquesindetectionofmethylmercuryprospectsinthisfieldarealsopresented.Keywordsmethylmercurychromatographyspectrometryhyphenatedinstrumentaltechniquessensor、1-3。。23、45678、。、202、426、5192、5449、236214.536。。、、、910。2、、。0.2mg/kg11。。GHZB1-1999CH3HgCl1.0μg/L0.5mg/kgGB2762-2005。、12、、、。。113、。。。。。、。。、。、、、。。。14。2。GB/T14204-9315。1L10mg/L。。0312。。80AES、AFS、ICP-MSHPLC。2.1。、、、。16。、17。、。。。、、。。。。SPE、、、18。SPMEWaterlooPawliszyn1990。、、、、、。SPME、、SPME。19。SDMESPME。SDME20。。2.2。、NaBEt4、NaBPr4、NaBPh4。2.32.3.1、。。21564nm。2.3.2。、。、、、13123。、、。。22、0.12ng/gCV=3.86%。。HPLC23。HPLCGCGC、、0.25ng。。24、、。HPLC、。25。50cm75μmL-。2.3.3。。2000GC-MS、GC-ICP-MSHPLC-ICP-MS。GC-MS。GC-MS、、。OviedoGiuseppeCentineoJ.IgnacioGarciaAlonsoGC-MS。201Hg。。ICP-MS、、、。GC-ICP-MSpg。Jitaru.PAdams.FC26。GC-MS。HPLCGCHPLC。HPLC-ICP-MS。27、。0.22mmHPLC-ICP-MS。、、。2.3.428-30。RibeiroF30。23123CH3Hg+、、、CH3Hg+。、、、。Hg2+、31-36。3738。CH3Hg+。CH3Hg+Hg2+S-/O-/S/NHg2+CH3Hg+Hg2+CH3Hg+39-45。Ahn39Hg2+CH3Hg+2Hg2+CH3Hg+11。2。11Fig.1Hydrolysismechanismofprobe1byHg2+andCH3Hg+inwater2aHg2+1bHg2+CH3Hg+1Fig.2aThetime-dependentfluorescencechangeacquiredfora2∶1mixtureofprobe1andHg2+bThefluorescenceintensitychangeofprobe1asafunctionofequiv.ofHg2+andCH3Hg+InjaeShinJinsungTae40RT13。RT1Hella。Hg2+CH3Hg+CH3Hg+Hg2+。331233RT1Fig.3Hg2+-induceddesulfurizationofRT1Torroba413/Hg2+Hg2+CH3Hg+4CH3Hg+5Hg2+443Hg2+Fig.4Methylation/demethylationofHg2+by33Hg2+CH3Hg+CH3Hg+。。CH3Hg+CH3Hg+4TorrobaCH3Hg+Hg2+CH3Hg+。4、。。。。1WangQRKimDDionysiouDDetal．Sourcesandremediationformercurycontaminationinaquaticsystems-aliteraturereviewJ．EnVironPollut2004131323-336．2ClarksonTWMagosLMyersGJ．Thetoxicologyofmercury-currentexposuresandclinicalmanifestationsJ．NEnglJMed20033491731-1737．3DavidsonPWMyersGJCoxCetal．Effectsofprena-talandpostnatalmethylmercuryexposurefromfishcon-sumptiononneurodevelopmentJ．JAmMedAssoc1998280701-707．4MalmO．MercurytoxicityintheAmazoncontrastsensitivityandcolordiscriminationofsubjectsexposedto4312mercuryJ．EnvironRes19987773-78．5PetkewichR．2003blackoutcleanedtheairJ．EnvironSciTechnol200135441A．6MasonRPSullivanKA．PublicationsLakemichiganmassbalancestudyJ．EnvironSciTechnol199731942-947．7Von-BurgR．InorganicmercuryJ．JApplToxicol199515483-493．8ClarksonTWMagosLMyersGJ．Thetoxicologyofmercury—currentexposuresandclinicalmanifestationsJ．NEnglJMed20033491731-1737．9LiuGCaiYPhilippiTetal．DistributionoftotalandmethylmercuryindifferentecosystemcompartmentsintheEvergladesImplicationsformercurybioaccumulationJ．Environ．Pollut2008153257-265．10LiuGCaiYKallaPetal．Mercurymassbudgetesti-matesandcyclingseasonalityintheFloridaevergladesJ．Environ．Sci．Technol2008421954-1960．11．J．200432117-22．12．J．2008353181-187．13．J．199226247．14．J．199327166-169．15GB/T14204-93．S．16WestG．Determinationofalkylmercuryinseawaterinthenanogramperliterlevelbygaschromatography/at-mosphericpressureheliummicrowave-inducedplasmae-missionspectrometryJ．ActaChemScand19662082131-2137．17．—J．199196350-352．18MunozJGallegoMValcarcelM．Simultaneousspeciationofmonomethylmercuryandmonoethylmercurybyaqueousphenylationandpurge-and-trappreconcentrationfollowedbyatomicspectrometrydetectionJ．JChromatogrA200410551-2185-190．19．J．200432121667-1672．20GilSFragueiroSLavillaI．Determinationofmethylmer-curybyelectrothermalatomicabsorptionspectrometryu-singheadspacesingle-dropJ．SpectrochimActaPartB2005601145-150．21．J．2000263295-290．22．J．19988291-93．23．-J．199523101168-1171．24BloomCJ．AlkylmercuryspeciesintheequatorialPacificJ．FishAquatSci1989461131-1140．25．J．20063343-45．26JitaruPAdamsFC．Speciationanalysisofmercurybysolid-phasemicroextractionandmulticapillarygaschromatographyhyphenatedtoinductivelycoupledplasmatime-of-flight-massspectrometryJ．JChromatogrA2