重组人HER2蛋白的纯化及抗HER2人源性抗体的初步筛选

中南大学硕士学位论文重组人HER2蛋白的纯化及抗HER2人源性抗体的初步筛选姓名：刘孝荣申请学位级别：硕士专业：病理学与病理生理学指导教师：贺智敏20050501重组人HER2蛋白的纯化及抗HER2人源性抗体的初步筛选作者：刘孝荣学位授予单位：中南大学参考文献(79条)1.参考文献2.SidoniA.CavaliereA.BellezzaGBreastcancerinyoungwomen:clinicopathologicalfeaturesandbiologicalspecificity2003(04)3.SlamonDJ.ClarkGM.WongSGHumanbreastcancer:correlationofrelapseandsurvivalwithamplificationoftheHER-2/neuoncogene19874.MenardS.BalsariA.CasaliniPHER-2-positivebreastcarcinomasasaparticularsubsetwithpeculiarclinicalbehaviors2002(02)5.RevillionF.BonneterreJ.PeyratJPERBB2oncogeneinhumanbreastcanceranditsclinicalsignificance1998(06)6.ShouJ.MassarwehS.OsborneCKMechanismsoftamoxifenresistance:increasedestrogenreceptor-HER2/neucross-talkinER/HER2-positivebreastcancer2004(12)7.PietrasRJ.ArboledaJ.ReeseDMHer2tyrosinekinasepathwaytargetsestrogenreceptorsandpromoteshormone-independentgrowthinhumanbreastcancercells1995(12)8.AkiyamaT.SudoC.OgawaraHTheproductofthehumanc-erbB-2gene:a185-kilodaltonglycoproteinwithtyrosinekinaseactivity1986(4758)9.BargmannCI.HungMC.WeinbergRATheneuoncogeneencodesanepidermalgrowthfactorreceptor-relatedprotein1986(6050)10.CoussensL.Yang-FengTL.LiaoYCTyrosinekinasereceptorwithextensivehomologytoEGFreceptorshareschromosomallocationwithneuoncogene1985(4730)11.KlapperLN.GlatheS.VaismanNTheErbB-2/HER2oncoproteinofhumancarcinomasmayfunctionsolelyasasharedcoreceptorformultiplestroma-derivedgrowthfactors1999(09)12.Graus-PortaD.BeerliRR.DalyJMErbB-2,thepreferredheterodimerizationpartnerofallErbBreceptors,isamediatoroflateralsignaling1997(07)13.SlamonDJ.GodolphinW.JonesLAStudiesoftheHER-2/neuproto-oncogeneinhumanbreastandovariancancer1989(4905)14.BerchuckA.KamelA.WnitakerROverexpressionofHER-2/neuisassociatedwithpoorsurvivalinadvancedepithelialovariancancer15.KernJA.SchwartzDA.NordbergJEp185neuexpressioninhumanlungadenocarcinomaspredictsshortenedsurvival1990(16)16.ParkJB.RhimJS.ParkSCAmplification,overexpression,andrearrangementoftheerbB-2protooncogeneinprimaryhumanstomachcarcinomas1989(23)17.QuaglinoE.IezziM.MastiniCElectroporatedDNAvaccineclearsawaymultifocalmammarycarcinomasinHer-2/neutransgenicmice200418.MenardS.PupaSM.CampiglioMBiologicandtherapeuticroleofHER2incancer2003(42)19.SpencerKS.Graus-PortaD.LengJErbB2isnecessaryforinductionofcarcinomacellinvasionbyErbBfamilyreceptortyrosinekinases2000(02)20.MenardS.BalsariA.CasaliniPHER-2-positivebreastcarcinomasasaparticularsubsetwithpeculiarclinicalbehaviors200221.TanM.YaoJ.YuDoverexpressionoftheC-erbB-2geneenhancedintrinsicmetostasispotentialinhumanbreastcancercellswithoutincreasingtheirtransformationabilities1997(06)22.MiguelAM.Rosanasáez.ElizabethERNH2-terminaltruncatedHER2breastcancer200223.StreckfusC.BiglerL.DellingerTThepresenceofsolublec-erbB-2insalivaandserumamongwomenwithbreastcarcinoma:apreliminarystudy200024.AliSM.LeitzelK.ChinchilliVMRelationshipofserumHER-2/neuandserumCA15-3inpacientswithmetastaticbreastcancer2002(08)25.KonoK.TakahashiA.SugaiHDendriticcellspulsedwithHER-2/neu-derivedpeptidescaninducespecificT-cellresponsesinpatientswithgastriccancer2002(11)26.DisisML.SchiffmanK.GuthrieKEffectofdoseonimmuneresponseinpatientsvaccinatedwithanher-2/neuintracellulardomainprotein--basedvaccine2004(10)27.LeeJY.KimDH.ChungYComparisonoftheantitumorefficaciesofHer-2/neuDNAvaccinesinducingcontrastingIgGimmunitybutcomparableCTLactivityinmice2003(5-6)28.VogelCL.CobleighMA.TripathyDEfficacyandsafetyoftrastuzumabasasingleagentinfirst-linetreatmentofHER2-overexpressingmetastaticbreastcancer2002(03)29.SlamonDJ.Leyland-JonesB.ShakSUseofchemotherapyplusamonoclonalantibodyagainstHER2formetastaticbreastcancerthatoverexpressesHER22001(11)30.GuptaP.SahaiV.BhatmagarREnhancedexpressionoftherecombinantlethalfactorofBacillusanthracisbyfed-batchculture2001(04)31.YuL.DengKP.YuCACloning,genesequencing,andexpressionofthesmallmolecularmassubiquinone-bindingproteinofmitochondrialubiquinol-cytochromeCreductase1995(43)32.DisisML.GralowJR.BernhardHPeptide-based,butnotwholeprotein,vaccineselicitimmunitytoHER-2/neu,anoncogenicself-protein1996(09)33.EssermanLJ.LopezT.MontesRVaccinationwiththeextracellulardomainofp185neupreventsmammarytumordevelopmentinneutransgenicmice199934.LilieH.SchwarzE.RudolphRAdvancesinrefoldingofproteinsproducedinE1998(05)35.SijwaliPS.BrinenLS.RosenthalPJSystematicoptimizationofexpressionandrefoldingofthePlasmodiumfalciparumcysteineproteasefalcipain-22001(01)36.VarnerinJP.ChungCC.PatelSBExpression,refolding,andpurificationofrecombinanthumanphosphodiesterase3B:definitionoftheN-terminusofthecatalyticcore200437.ArakawaT.TsumotoKTheeffectsofarginineonrefoldingofaggregatedproteins:notfacilitaterefolding,butsuppressaggregation200338.KrainerM.BrodowiezT.ZeillengerRTissueexpressionandserumlevelsofHER-2/neuinpatientswithbreastcancer199739.ClarkGM.McguireWLFollow-upstudyofHER-2/neuamplificationinprimarybreastcancer199140.MolinaMA.Codony-ServatJ.AlbanellJTrastuzumab(herceptin),ahumanizedanti-Her2receptormonoclonalantibody,inhibitsbasalandactivatedHer2ectodomaincleavageinbreastcancercells200141.McCaffertyJ.Griffiths