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3142004JOURNALOFBEIJINGUNIVERSITYOFCHEMICALTECHNOLOGYVol.31,No.420043(,100029):,;,pH,pH,pH710,015%1%,,58%:;;;;:Q93919:2003211220:,1974,3E2mail:sunzs0927@sina.com.cn,100[1](rHSA),rHSA[223],,[2],,,,pH[4][5]pH[6],pH(2031,pH310710),pH1111PichiapastorisGS115/rHSA,112BMGY/BMMYPTM1Invitrogen[7]PBM40g/L,H3PO4(85%)2617g/L,CaSO42H2O016g/L,K2SO4915g/L,MgSO47H2O718g/L,KOH216g/L,PTM12mL/L,Biotin0100004g/L,0100019g/L,pH11330,pH28%pH310,410,510,610,710,4hpHpH,21,24,2830,pH61012h015%180r/min,500mL(baffledflask),50mL114rHSASDS2PAGEGelpro3112211rHSABMGY/BMMYBMGY/BMMY,,rHSA(1),Gelpro311,rHSA31%,rHSABMGY/BMMY,PBM1,2,3,4GS115/rHSABMGY/BMMY24,48,72,96h;MHSA()1BMGY/BMMYrHSAFig.1SDS2PAGEresultofrHSAexpressedinBMGY/BMMYmedium212rHSArHSA,YP(Yeastex2tract,peptone12),(2),YP1,248,72h;3,40125%,Y,0150%P48,72h;5,60150%Y110%P48,72h;7,8110%Y210%P48,72h,;9,10YP7,8,2YPrHSAFig.2SDS2PAGEanalysistoinvestigatetheeffectofaddingYPondegradationofrHSAinPBMmediumrHSA,YP,YP015g/L110g/L,YPrHSA,YPYP,(rHSA27%29%),YPrHSAYPYP,rHSA,rHSA3,2g/L(NH4)2SO4YP,YP,,1,2,32g/L(NH)2SO424,48,72h;4,5,615g/L;7,8,9YP0150%110%3rHSAFig.3Effectofdifferentnitrogen2sourcesupplementsonrHSAdegradation213pHrHSApHrHSA,PBM015%1%YP4,pH37,rHSA,pHTo2moshipH[8]Mehmedalija[5]pHpH214rHSAMehmedalija,,,PBM015%1%YP,(0120041,2pH31048,72h;3,4pH510;5,6pH610;7,8pH710;9,10pH4104pHrHSAFig.4EffectofmediumpHonrHSAdegradation5),rHSA1,2,32124,48,72h;4,5,624;7,82848,72h;9,103048,72h5rHSAFig.5Effectofinduce2temperatureonrHSAdegradation3,PBMrHSA58%(6),Kaoru90%,,ABY,A,YB2B[9]Apep4,,1,2,3,424,48,72,96h6rHSApH710,YP015%110%Fig.6ExpressionofrHSAintheoptimalconditionswithpH710andYP(015%,110%)[1]JoanLinCereghino,JamesMCregg.Heterologouspro2teinexpressioninthemethylotrophicyeastpichiapas2toris[J].FEMSMicrobiologyReviews,2000,24:45-66[2]KaoruKorayashi,ShnobuKowar,TomoshiOhya.High2levelexpressionofrecombinanthumanserumalbuminfromthemethylotrophicyeastpichiapastoriswithmini2malproteaseproductionandactivation[J].JournalofBioscienceandBioengineering,2000,89(1):45-61[3],,,.PichiaPas2toris[J].,2001,28(3):6-11[4]WertenMWT,VandenBoschTJ,WindRD.High2yieldsecretionofrecombinantgelatinsbypichiapastoris[J].Yeast,1999,15(11):1087-1096[5]MehmedalijaJahic,MalinGustavsson,Ann2katrinJansen.Analysisandcontrolofproteolysisofafusionproteininpichiapastorisfed2batchprocess[J].JournalofBiotechnology,2003,102:45-53[6],.[M].:,1990[7],,,.Muts[J].,2003,22(1):12-15[8]Tomoshiohya,Masanorimorita,Masamimiura.High2levelproductionofprourokinase2annexinVchimerasinthemethyllotrophicYeastpichiapastoris[J].JournalofBioscienceandBioengineering,2002,94(5):467-473[9]HigginsDR,CreggJM.Methodsinmolecularbiology[M].Totowa:HumanapressInc,1997,81-94(15)114:lysts[J].IndEngChemRes,1993,32:1053-1060[7]Tops«eNY,DumesicJA,Tops«eH.Vanadia/titaniaforcatalystsforselectivecatalyticreductionofnitricoxidebyammonia:Studiesofactivesitesandformulationofcatalyticcycles[J].JCatal,1995,151:241-252[8]RonconiE,ForzattiP,GomezMartinJP,etal.Selec2tivecatalyticremovalofNOx:Amathematicalmodelfordesignofcatalystandreactor[J].ChemicalEngineeringScience,1992,47(9211):2401-2406[9]ReidRC,PrausnitzJM,PolingBE.Thepropertiesofgasesandliquids[M].FourthEdition.NewYouk:Mc2Graw2Hill,1987[10].[M]..:,2000,102-103[11]DwirediPN,Upadhyay.Particle2fluidmasstransferinfixedandfluidizedbeds[J].IndEngChemProcessDesDevelop,1977,16:157[12].[M].:,1994,284,294EffectofmasstransferbehaviouronselectivecatalyticreductionofNOxwithNH3overV2O5/TiO2palletcatalystQuHong2xiaZhongQin(SchoolofChemicalEngineering,NanjingUniversityofScienceandTechnology,Nanjing210094,China)Abstract:V2O5/TiO2catalysts,whichwereappliedtotheselectivecatalyticreductionofNOxwithNH3inafixedbedreactor,werepreparedbyimpregnating.ReactionofNH3reducedtoNOxisfirstorderforthepalletcatalyst,andtheinternaldiffusionsignificantlyinfluencesthereactionrateandNOxconversion.TheoverallNOxconversioncanbeexpressedasanexplicitfunctionofthespacevelocityandotherparameters,andasimpleanalyticsolutionwasgiven.Agoodagreementbetweentheexperimentandmodelresultswasobtained.Itisshownthatthemodelequationcansuccessfullyreflecttheconversiontrend,andprovidesatheoreticalbasisfordesigningacatalyticreactor.Keywords:denitrification;palletcatalystofV2O5/TiO2;selectivecatalyticreduction;masstransfermodel()(11)RepressionofrHSAdegradationduringfermentationwithtransgenicyeastpichiapastorisSunZhan2shengChenJin2chun(CollegeofLifeScienceandTechnology,BeijingUniversityofChemicalTechnology,Beijing100029,China)Abstract:Oneobviousproblemforoverexpressionrecombinanthumanserumalbumin(rHSA)withthetrans2genicyeastpichiapastorisisdegradationofthepurposeproteinsduringfermentation.Minimizationofdegrada2tionwasthereforeinvestigatedforimprovingtheyieldofintactrHSA.Theresultsshowedthatanadditionof015%yeastextractand1%peptone(YP)tothemediumwaseffectivetodecreasethedegradationoftherHSA.CultureconditionofhighpHwasalsosubstantiallyreducedthedegradation.Howevertherewaslittleef2fectbychangingthetemperatureduringthemethanolfeedingphase.BycombiningtheoptimalpHof710withtheadditionofYP(015%,110%),the58%inta