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##*a,b(a.;b.,030801):,,,,,,,,:;;:Q813.2:A:100727146(2006)0220209205CellFusionTechnique:itsDevelopmentandApplicationsHUONai2ruia,HANKe2guangb(ShanxiAgriculturalUniversitya.CollegeofFoodScienceandEngineering;b.CollegeofAnimalScienceandTechnology,Taigu030801,Shanx,iChina)Abstract:Cellularengineeringisoneofthefourtechniquesofbiotechnology,andasthecoreofcellularengineering,thecellfusiontechniquehasacquiredoutstandingachievementsinmanyfieldssuchasagriculture,medicineandenvi2ronmentalprotection.Itsapplicationisstillincreasinginnumber.Theimprovementofthecellfusiontechniqueinvolvesthefusionagent,newmethodsandthecellsusedinfusion.Nownewcellfusionmethodsareresortingtothecombinedusageofphysicalandchemicalmethodstodevelopasimpleandconvenient,easyquantificationandatthesametimecanincreasethefusionrate.Alltheseaspectswerediscussedinthispapercenteredaroundthehistoryofthecellfusiontech2nique.Keywords:cellfusiontechnique;development;application,207080,,,,,,15220064ACTALASERBIOLOGYSINICAVo.l15No.2Apr.2006*:2005203210;:2005209222:(04105523);(20041094):(1972)),,,,.()sxndkgh@163.com[122],11.1(),(),(cellfusion)(cellhybridization)(somatichybridization)[1],,1.2,,,,,,,,,[1],2,,,,,,,,,PEG,,,/0PEG(50%~55%),PEG,,,,,,,2.119,/0Muller(1838),[3],,1875Lange(),2.2Harrison(1907),Lambert(1912)1960,Barski,2.3195821960(Okada)(ETC),ETC,,HVJETCETC,37e5min,pH21015,,,2.4,,,1965,Harris,,,,Littlefield(1964),1967WeiseGreen,,WatkinsKo2prowski1970Ruddle1970Ladda,[4],,HVJ,2.51937,Mi2chel0.5mol/L,,1960Cocking,1972Carlson2070,2.61975,FerenczyPEG,,,2.7PEG,PEG(1974),(PEG)Ca2+,,,,/0,Pontecorvo(1975)PEG1976Davidson,PEG,,,1975AhkonoPEG1976DudusDavey(1972)Vasil(1977)[425]1975,,))),PEGDavidson1976PEG,,,,2.82070,,Carter1967B(cytochalasinB,CB)LPrescott1972CB,2112:,,;,;,1982,Ferenczy,,[1,4,6]2.9,PEG,PEG,PEG(50%~55%),PEG2.9.12080HalfmanZmimermann(19821983),,:,PEG100;,;;,[728]2.9.22080,(),,,,,,,[1,3,4]19871989,,,,[9]PEG,,,,,,,,,,,,,,,(poticaltweezers),,,,[10]2.9.3,,[11212],,B2080,Zmimermann,,,,2.9.4,2080,,,,,,,,,,,,,,,,,[13215]2.9.5(C,C,gamma2fusion)21215,6G,,,,,[1,9]CX,,[16],,,,,,,,,PEG;,,,,,,,3,,,,,,References[1].[M].:,2003:3221.LIZhi2yong.CellularEngineering[M].Beijing:SciencePress,2003:3221.[2].[M].:,2003:1212.LUOLi2xin.CellFusionTechniqueandApplication[M].Bei2jing:ChemicalIndustryPress,2003:1212.[3]JENABP.InsightsonMembraneFusion[J].CellBiologyIn2ternationa,l2000,24(11):7692771.[4],,.[M].:,2003:5219.LUOLi2xin,PanL,iZHENGSui2ping.CellEngineering[M].Guan2gzhou:SouthChinaUniversityofTechnologyPress,2003:5219.[5]JOINERMC,BARRYDM,OLEGVB,eta.lGrayLaborato2ryResearchAnnualReport[M].CellandMolecularBiophysics,1998:34240.[6]GROSOVSKYAJ.Radiation2induceMutationsinUnirradiatedDNA[J].ProcNatlAcadSciUSA,1999,96:534625347.[7]HAYASHIT,TANAKAH,TANAKAJ,eta.lImmunogenicityandTherapeuticEfficacyofDendritic2TumorHybridCellsGener2atedbyElectrofusion[J].ClinicalImmunology,2002,104(1):14220.[8]RAMOSC,BONENFANTD,TEISSIEJ,eta.lCellHybridiza2tionbyElectrofusiononFilters[J].AnalyticalBiochemistry.2002,302(2):2132219.[9]QIPEIMIN,TODOROVAN,TODOROVAT,eta.lActionPotentialsPassSeveredEarthwormMGASegmentsReconnectedbyLaserandElectricFieldPulses[J].BrainResearchBulletin,1995,37(2):1892192.[10]STEUBINGRW,CHENGS,NUMAJIRIY,eta.lLaserIn2ducedCellFusioninCombinationWithOpticalTweezers[J].TheLaserCellFusionTrapCytometry,1991,12:5052510.[11]ZIMMERMANNU,HANNIGK,etal.BiotechnologyinSpace:PotentialsandPerspectives[J].Biotechnology,1988,6b:6392672.[12]JONGKINDJF,VISSERP,VERKERKA,eta.lCellFusioninSpace:PlasmaMembraneFusioninHumanFibroblastsdur2ingShortTermMicrogravity[J].AdvanceinSpaceResearch,1996,17(6~7):21225.[13]SCHETTINOG.LowDoseHypersensitivityinChineseHamsterV79CellsTargetedwithCountedProtonsusingaCharged2parti2cleMicrobeam[J].RadiationResearch,2001,156(5Pt1):5262534.[14]LIUFeng,WANGYu2gang,XUEJian2ming,eta.lTransmis2sionMeasurementBasedonSTMObservationtoDetectthePen2etrationDepthofLow2energyHeavyIonsinBotanicSamples[J].RadiationMeasurements,2003,37(1):9214.[15],.[J].,2003,23(4):57261.YUANCheng2ling,YUZeng2liang.TheGrandProgressofLowEnergyIonBeaminLifeSciences[J].ChinaBiotechnology,2003,23(4):57261.[16]HEITK,WULJ,LIUSX,eta.lMutagenicEffectsofaSin2gleandanExactNumberofAlphaParticlesinMammalianCells[J].ProcNatlAcadSciUSA,1997,94:376523770.2132: