HPLC法测定阿奇霉素在水溶液中的降解动力学

:2007207213:(19832),(),,,[e-mail];(19652),(),,,,,Tel.[phone],[e-mail]:100622858(2008)0620475205HPLC,,,,,(,110016):,,HPLCpH,pH(pHm)6141;,;pH;pH:;;;:R917;R94:A(azithromycin,1),,,[1-2],,pH,Fig11Structureofazithromycin1(JascoUV22075JascoPU22080ChromPass,Jasco),PHS23CpH(),AdventurerAR1140(),HH22()(0504077,),(130352200304,),(,),(,)2211HPLC21111:DiamonsilC18(416mm250mm,5m);:45mmolL-1(pH712)2(5545);:210nm;:40;:110mLmin-1,(2),2000211121g,,100mL,25620086JournalofShenyangPharmaceuticalUniversityVol125No16Jun12008p1475©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(),(A),,:A=118698+111510-2,r=019999,015510gL-1ANot2degradated;BAfterdegradationFig12HPLCchromatogramsofazithromycin2111380100120mg,,100mL,,21112,110gL-120L,,,9916%,RSD0184%21114110mL10mL,,20L,6,,RSD0179%21221211pH,100mL,pH(011molL-1),,,pH21663124417851646103614171117151,10gL-12mL60,,1mL10mL,,HPLC,k[3],(ln(/0))(t)34,ln(/0)2t(r0199)pHk55,pH6141,k,pH6141pH,kFig13DecayplotsofazithromycinatpH4178,5164,6103,6141,7151and7111inaqueoussolutions67425©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(011molL-1),,,10gL-1;2mL,4050607021211,4k,6,,klnk1/T,7(Arrhenius)6Ea=-R(R),4070,86186kJmoL-1254,254k316710-5h-1219510-6h-1,t019=011054/k,70605040254t019112921588178Fig16EffectsofdifferenttemperaturesondegradationconstantsofazithromycinFig17Arrheniusplotfordegradationofazithromycininaqueoussolution211961191701490183d21213,100mL,pH610(011molL-1),,,10gL-1,NaCl,012015110,2mL,602121160,k,8,,21214,100mL,pH610011molL-17746:HPLC©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[4],,,,,(2),b1,6,,,4701154119,,c1,,8,,,d1,,3,4,HPLC,,,pH6141;,407086186kJmoL-1;3,,pH,pH,,,87425©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[1]JARURATANASIRIKULS,HORTIWAKULR,TAN2TISARASARTT,etal.Distributionofazithromycinin2tobraintissue,cerebrospinalfluid,andaqueoushumoroftheeye[J].AntimicrobAgentsChemother,1996,40:825-826.[2]RETSEMAJ,GIRARDA,SCHELKLYW,etal.Spec2trumandmodeofactionofazithromycin(CP262,993),anew152membered2ringmacrolidewithimprovedpo2tencyagainstgramnegativeorganisms[J].AntimicrobAgentsChemother,1987,31:1939-1947.[3].[M].5.:,2003:7-215.[4],.HPLC[J].,2006,11(31):672-674.DegradationkineticsdeterminationofazithromycininaqueoussolutionbyHPLCXUShi2zhao,QIANYi2xin,XUShu2ying,LIHe,SUNYing2hua,HEZhong2gui(SchoolofPharmacy,ShenyangPharmaceuticalUniversity,Shenyang110016,China)Abstract:ObjectiveTostudythedegradationkineticsofazithromycininaqueoussolution.MethodsTheHPLCmethodandconstanttemperaturemethodwereusedtodeterminethedegradationkineticparametersofazithromycinatdifferentpH,temperatures,ionicstrengths,andbuffers.ResultsDegradationofazithromycininaqueoussolutionfollowedfirst2orderkinetics,andthepHmforazithromycinwas6.41.Thedegradationrateincreasedwithionicstrengthandtemperature.AzithromycinwasrelativelystableinPBSthantheotherbuffers.ConclusionsDegradationrateofazithromycinisrelatedwithpH,specieofthebuffer,ionicstrengthandtemperature.Keywords:azithromycin;kinetics;stability;degradation9746:HPLC©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.