veriti PCR使用手册

AppliedBiosystemsVeriti™ThermalCyclerUserGuideAppliedBiosystemsVeriti™ThermalCyclerUserGuide©Copyright2008,2010AppliedBiosystems.Allrightsreserved.GeneralLaboratoryUse.Informationinthisdocumentissubjecttochangewithoutnotice.AppliedBiosystemsassumesnoresponsibilityforanyerrorsthatmayappearinthisdocument.APPLIEDBIOSYSTEMSDISCLAIMSALLWARRANTIESWITHRESPECTTOTHISDOCUMENT,EXPRESSEDORIMPLIED,IN-CLUDINGBUTNOTLIMITEDTOTHOSEOFMERCHANTABILITYORFITNESSFORAPARTICULARPURPOSE.INNOEVENTSHALLAPPLIEDBIOSYSTEMSBELIABLE,WHETHERINCONTRACT,TORT,WARRANTY,ORUNDERANYSTATUTEORONANYOTHERBASISFORSPECIAL,INCIDENTAL,INDIRECT,PUNITIVE,MULTIPLEORCONSEQUENTIALDAMAGESINCONNECTIONWITHORARISINGFROMTHISDOCUMENT,INCLUDINGBUTNOTLIMITEDTOTHEUSETHEREOF.NOTICETOPURCHASER:TheVeriti™ThermalCycleriscoveredbyUSpatentclaimsandcorrespondingclaimsintheirnon-UScounterparts.Norightisconveyedexpressly,byimplication,orbyestoppelunderanyotherpatentclaim,suchasclaimstoapparatus,reagents,kits,ormethodssuchas5’nucleasemethods.Furtherin-formationonpurchasinglicensesmaybeobtainedbycontactingtheDirectorofLicensing,AppliedBiosystems,850LincolnCentreDrive,FosterCity,California94404,USA.TRADEMARKS:Applera,AppliedBiosystems,AB(Design),ABIPRISM,BigDye,andGeneAmp,areregisteredtrademarksandMicroAmp,VeriFlex,andVeritiaretrademarksofAppliedBiosystemsoritssubsidiariesintheU.S.and/orcertainothercountries.AmpliCycle,AmpliTaq,andAmpliTaqGoldisaregisteredtrademarkofRocheMolecularSystems,Inc.Allothertrademarksarethesolepropertyoftheirrespectiveowners.PartNumber4375799Rev.E06/2010ContentsFrontMatteriiiVeriti™ThermalCyclerUserGuidePrefaceHowtoUseThisGuide.............................................viiHowtoObtainMoreInformation......................................viiiHowtoObtainSupport.............................................viiiSafetyandEMCComplianceInformationSafetyConventionsUsedinThisDocument.............................xiiSymbolsonInstruments............................................xiiiSafetyLabelsonInstruments........................................xivGeneralInstrumentSafety...........................................xvChemicalSafety..................................................xviChemicalWasteSafety............................................xviiElectricalSafety..................................................xviiPhysicalHazardSafety............................................xviiSafetyandElectromagneticCompatibility(EMC)Standards...............xviiChapter1SystemOverviewIntroducingtheVeriti™ThermalCycler................................1-2UsingtheTouchscreen............................................1-3MenuOverview..................................................1-6AboutRunMethods..............................................1-8AboutFolders..................................................1-15Chapter2GettingStartedInstallingtheVeriti™ThermalCycler..................................2-2BeforeYouStart..............................................2-2EnvironmentalRequirements....................................2-3Materials....................................................2-3UnpackingtheVeriti™System...................................2-3SettingUptheVeriti™System...................................2-4ConnectingtoaNetworkPrinter.................................2-5ConnectingTogetherMultipleInstruments.........................2-5ivVeriti™ThermalCyclerUserGuidePoweringOn.................................................2-6SettingUptheVeriti™ThermalCycler.................................2-7RecommendedInstrumentSettings...............................2-7SettingUpInstrumentSecurityandUserAccounts...................2-9AddingUsers................................................2-10SettingOtherOptions............................................2-14Chapter3PerformingaRunSelectingDisposables.............................................3-2Disposablesfor96-WellVeriti™ThermalCyclers.....................3-2Disposablesforthe384-WellVeriti™ThermalCycler..................3-4Disposablesforthe60-WellVeriti™ThermalCycler...................3-4LoadingSamplesintotheInstrument.................................3-5LoadingSamplesinVeriti™96-WellThermalCyclers..................3-5LoadingSamplesintheVeriti™384-WellThermalCycler...............3-6LoadingSamplesintheVeriti™60-WellThermalCycler................3-7CreatingaNewRunMethod........................................3-8EditingDetails..................................................3-10PerformingaRun................................................3-18MonitoringaRun................................................3-21ViewingandPrintingtheRunReport.................................3-25RemovingtheSamplesfromtheInstrument...........................3-25Chapter4TroubleshootingTroubleshooting..................................................4-2ReturninganInstrumentforService..................................4-3Chapter5UtilitiesUsingGeneAmp®PCRSystem9700RunMethods......................5-2UpgradingSystemFirmware........................................5-5Calculat