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doi10．3969/j．issn．1002－7386．2012．02．058··071000【】HPVDNA【】R713．4【】A【】1002－7386201202－0259－03。。205012。。1、1．1、、HPVⅡ。1．23cm。。、。。1．3HPV2004“”ⅠCINIⅡⅢ。。CINⅠCINⅡCINⅢ、。22．11928Papanicolaou。、。180%32、15%～30%。2．2。。ASCUS9．5%6．3%LSIL7．8%5．3%4。2001TheBethesdasys-temTBSTBS、atypicalsquamouscellsofundeterminedsignificanceASCUS、lowgradesquamousintraepitheliallesionLSIL、highgradesquamousin-traepitheliallesionHSIL、squamouscellcarcinomaSCCAtyp-icalglandularcellsofundeterminedsignificanceAGUSadenocarcinoma。TBS。3HPV-DNA3．1HPVHPV———HPV。20062007HPVHPV。、HPVHPVHPV95220121342HebeiMedicalJournal2012Vol34JanNo.2。。HPV80HPVHPV3520HPVHPV6、11、42、43、44HPV16、18、31、33、35、39、45、51、52、56、58、59、68。HPV1618。HPV1650%HPV1814%HPV458%HPV315%HPV23%。HPVHPV1651%56%39%HPV18。99．8%HPVHPV。48%HPV。HPVHPV5。3．2HPV-DNAHPVHPV。HPV。3HPV-DNA、DNA。PCRHPV-DNA。PCRHPV-DNAHC-ⅡHPV-DNA6。3．3HPV-DNAHPV-DNA。HC-2HPV-DNAASCUS7。TCTTCTASCUSHPV。4DNADNA2080～90、。、。。DNADNA。DNA8。DNADNA。DNADNADNADNA、。DNA。TBSDNADNA＞2．55c。ASCUSLSILDNAHPV。。DNA。1991DNA。。55．1argyrophilicnucleolarorganizerre-gionsAgNORsNORrRNADNA13-15p21p25910rRNArRNA。、、AgNOR11。AgNORrRNA、。AgNOR、、。AgNORsAgNORsAgNORs12。。5．2Ki67CD44v6Ki67。G0G1、S、G2M。95%G0Ki67。Ki67CINCINPCRHPVDNA。13Ki67、CINKi67CIN。DNAKi67。CD44v6CD44CD4414。CD44v6、、06220121342HebeiMedicalJournal2012Vol34JanNo.2、1516。CD44v6CD44v6。6→→HPV-DNA→→→HPV-DNAHPVHPV1718。HPV20～3025%～30%19。8～10355%～10%HPVHPV20。。HPV。HPV-DNA。。DNA。“”。、、。。5%～6%。。。、HPV-DNA、、DNA、。1ParkinDMBrayFFerlayJetal．Globalcancersstatistics．CACancerJClin20055574-108．2．．200516103-105．3．．200123309-312．4BiscottiCVBrienDLGeroMAetal．ThinlayerPaptestvsconvention-alPapsmearAnalysis100splitsamples．RepMed2002479-13．5．．200237129-131．6SandriMTLentatiPBeniniEetal．ComparisonoftheDigeneHC2As-sayandtheRocheAMPLICOR．JClinMicrobiol2006442141-2146．7SchiffmanMWheelerCMDasguptaAetal．AcomparisonofaprototypePCRassayandhybridcapture2fordetectionofcarcinogenichumanpap-illomavirusDNAinwomenwithequivocalormildlyabnormalPapanico-lausmears．AmClinPathol2005124722-732．8．HPV-DNATCT．20101028-30．9KoutskyLA．Epidemiologyofgenitalhumanpapillomavirusinfection．AmJMed19971023-8．10SmithRCrockerJ．Evaluationofnucleoarorganizerregion-associatedproteinsinbreastmalignancy．Histopathology198812113．11WilliamsMAKleinschmidtJAKrohneGetal．Argyrophilicnucleolarandnucleolarproteinsofxenopuslaevisoocytesidentifiedbygelelec-trophroresis．ExpcellRES1982137341．12EganMJRaafatFCrockerJ．Necleolarorganizerregioninsmallcelltumousofchildhood．JPathol1987153275．13．31．20057814．14．DNA．1．2006．40-43．15．E-cadherinCD44v6DNA．200728907-909．16．CD44v6p16．20062145-4648．17．、．1．2004．2-6．18GroteHJFriedrichsNPomjanskiNetal．PrognosticsignificanceofDNAcytometryincarcinomaoftheuterinecervixFIGOStageBandII．AnalvCellPathol20012397-105．19AnHJChoNHLeeSYetal．Correlationofcervicalcarcinomaandpre-cancerouslesionswithhumanpapillomavirusHPVgenotypesdetectedwiththeHPVDNAchipmicroarraymethod．Cancer2003971672-1680．20HwangTSJeongJKParKMetal．DetectionandtypingofHPVgeno-typesinvariouscervicallesionsbyHPVoligonucleotidemicroaray．Gy-necolOncol20039051-56．2011－10－1116220121342HebeiMedicalJournal2012Vol34JanNo.2