常用荧光染料的激发和发射波长

染料名称Excitation(nm)Emission(nm)分子量备注信息ReactiveandconjugatedprobesHydroxycoumarin325386331SuccinimidylesterAminocoumarin350445330SuccinimidylesterMethoxycoumarin360410317SuccinimidylesterCascadeBlue(375);401423596HydrazidePacificBlue403455406MaleimidePacificOrange403551Luciferyellow425528NBD466539294NBD-XR-Phycoerythrin(PE)480;565578240kPE-Cy5conjugates480;565;650670akaCychrome,R670,Tri-Color,QuantumRedPE-Cy7conjugates480;565;743767Red613480;565613PE-TexasRedPerCP490675PeridininchlorphyllproteinTruRed490,675695PerCP-Cy5.5conjugateFluorX494520587GEHealthcareFluorescein495519389FITC;pHsensitiveBODIPY-FL503512TRITC547572444TRITCX-Rhodamine570576548XRITCLissamineRhodamineB570590TexasRed589615625SulfonylchlorideAllophycocyanin(APC)650660104kAPC-Cy7conjugates650;755767PharRedAlexaFluor系列荧光染料AlexaFluor350343442410AlexaFluor4054014211028AlexaFluor430434540702AlexaFluor488499519643QY0.92AlexaFluor500503525700AlexaFluor514517542714AlexaFluor532530555724QY0.61AlexaFluor5465615721079QY0.79AlexaFluor5555535681250QY0.1AlexaFluor568579603792QY0.69AlexaFluor594591618820QY0.66AlexaFluor6106106291285AlexaFluor6336326481200AlexaFluor6476526681300QY0.33AlexaFluor6606636911100AlexaFluor6806807021150AlexaFluor7006967191400AlexaFluor7507527761300AlexaFluor7907828041750Cy系列荧光染料Cy2489506714QY0.12Cy3(512);550570;(615)767QY0.15Cy3B558572;(620)658QY0.67Cy3.5581594;(640)1102QY0.15Cy5(625);650670792QY0.28Cy5.56756941128QY0.23Cy7743767818QY0.28核酸相关探针染料Hoechst33342343483616AT-selectiveDAPI345455AT-selectiveHoechst33258345478624AT-selectiveSYTOXBlue431480~400DNAChromomycinA3445575CG-selectiveMithramycin445575YOYO-14915091271EthidiumBromide493620394AcridineOrange503530/640DNA/RNASYTOXGreen504523~600DNATOTO-1,TO-PRO-1509533Vitalstain,TOTO:CyanineDimerTO-PRO:CyanineMonomerThiazoleOrange510530PropidiumIodide(PI)536617668.4LDS751543;590712;607472DNA(543ex/712em),RNA(590ex/607em)7-AAD5466477-aminoactinomycinD,CG-selectiveSYTOXOrange547570~500DNATOTO-3,TO-PRO-3642661DRAQ5647681,697413usableexcitationdownto488细胞功能探针Indo-1361/330490/4051010AMester.Low/HighCa++,Fluo-3506526855AMester.pH6DCFH5055355292'7'Dichorodihydrofluorescein,oxidizedformDHR505534346Dihydrorhodamine123,oxidizedform,lightcatalyzesoxidationSNARF548/579587/635pH6/9荧光蛋白QYBRPSY66H360442Y66F360508EBFP3804400.180.27monomerEBFP238344820monomerAzurite38344715monomerGFPuv385508T-Sapphire3995110.602625weakdimerCerulean4334750.622736weakdimermCFP4334750.401364monomerECFP4344770.153CyPet4354770.511859weakdimerY66W436485mKeima-Red4406200.243monomerTagCFP45848029dimerAmCyan14584890.7529tetramermTFP146249254dimerS65A471504MidoriishiCyan4724950.925dimerWildTypeGFP396,47550826k0.77S65C479507TurboGFP48250226k0.5337dimerTagGFP48250534monomerS65L484510Emerald4875090.68390.69weakdimer;(Invitrogen)S65T488511EGFP48850726k0.6034174weakdimerAzamiGreen4925050.7441monomerZsGreen1493505105k0.9140tetramerTagYFP50852447monomerEYFP51452726k0.615160weakdimerTopaz51452757monomerVenus5155280.575315weakdimermCitrine5165290.765949monomerYPet5175300.778049weakdimerTurboYFP52553826k0.531.65dimerZsYellow15295390.6513tetramerKusabiraOrange5485590.6031monomermOrange5485620.69499monomermKO5485590.6031122monomerTurboRFP55357426k0.6762dimertdTomato5545810.699598tandemdimerTagRFP55558450monomerDsRedmonomer556586~28k0.13.516monomerDsRed2(RFP)563582~110k0.5524mStrawberry5745960.292615monomerTurboFP60257460226k0.3526dimerAsRed2576592~110k0.2113tetramermRFP1584607~30k0.25monomerJ-Red5846100.208.813dimermCherry5876100.221696monomerHcRed1588618~52k0.030.6dimerKatusha58863523dimermKate(TagFP635)58863515monomerTurboFP63558863526k0.3422dimermPlum5906490.104.153mRaspberry5986250.1513monomer;fasterphotobleachthanmPlum其他探针Monochlorobimane380461226GlutathioneprobeCalcein496517623pH5Ex:Peakexcitationwavelength(nm)Em:Peakemissionwavelength(nm)QY:QuantumyieldBR:Brightness;Extinctioncoefficient*Quantumyield/1000PS:Photostability;timeto50%brightness(sec)