厌氧氨氧化微生物研究进展

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0,NH4+NO2-,。,NH4+,NO2-,(N2)。(Anammox)。。[1]。,。,,。、。,。11.1,,。,,1μm。,。1.2。1(cellwall),(cytoplasmicmembrane),pp(paryphoplasm)、(intracytoplasmicmembrande),Anammox(anammoxosome)、(riboplasm)、(nucleoid)、。a.b.1,,,(,215011):。。,。,,。。:;;;:X2:A:1674-4829(2009)02-0058-04:2008-11-17:(BK2002043),(Z964),(A0030702).:(1984-),,,,.ResearchProcessofAnammoxBacteriaLIXiang,YUANYi,HUANGYong,WANGYongAbstract:Anaerobicammoniumoxidation(ANAMMOX)isanovelprocessofnitrogenremovalthathasbeingdevelopedforafewyears.Anammoxbacteriaplaythemajorroleintheprocessofnitrogenremoval.Currently,agrowingnumberofscholarsbegantostudytheanammoxbacteria,whichhaveagreatsignificancefortheanammoxtechnologydevelopmentandextension.FromtheviewofcurrentANAMMOXresearch,thepaperintroducedtheanammoxbacteria'stype,structureandfunction.Keywords:Anammox;Anammoxbacteria;Anammoxosome;StructureandfunctionofbacteriaPPAnammoxAnammox22220094EnvironmentalScienceandTechnologyVol.22No.2Apr.20092221.3,,,“”[2]。,。,。2。RNase-goldRNA。。2pp,。,。,。33。,planctomycetes()。16Srnaplanctomy-cetes。planctomy-cetes,。[3],,,。,JAYNE[4],2,。,,。。。。,。,,,。,。TEM-EDX(Fe),,2。KstuttgartiensisFe,2Kuste3640kuste4480。Ecoli32%~48%Fe,Fe。Fe[5]。,。,Kstuttgartiensis10kDC[6],Fe。STROUS[7]ρ[Fe2(SO4)3]6.25mg/LFe2(SO4)3,Fe;(20%),Fe。,,。vanNIFTRIK[8]Fe0.83,Fe。2、,pH7.5~8.5,30~40℃。,STROUS98mg/L。TSUSHIMA[9]NO2-710mg/L,。NO2-。,NaClKCl。LIUST,10mg/L.KARTAL[10]SBR。,30g/L,WINDEY[11]。FISHKstuttgartiensis。33.1,、4。。。,1:5920094K.stuttgartiensisB.fulgidaScalinduaspanammoxoglobuspropionicus800nm800nm950nm1100nm61%±5%61%±5%56%±8%66%±6%[10][10][10][10]*CandidatusBrocadiaanammoxidansCandidatusKueneniastuttgartiensisCandidatusanammoxglobuspropionicusCandidatusBrocadiafulgidaanaerobicammonium-oxidizingPlanctomycetecquenviron-1CandidatusScalinduasorokiniiCandidatusScalinduawagneriCandidatusScalinduabrodaeNitrosomonaseuropaeaNitrosomonaseutropha[9][12][13][9][14][15][16]1:“*”O2,N2,。3.2Kstuttgartiensis,Banammoxidans,Bfulgida,anaerobicammonium-oxidizingPlanctomycetecquenviron-1anammoxoglobuspropionicus。5anammoxoglobuspropionicusBfulgida,,、。ScalinduasppO2[21]。Kstuttgartiensis,anammoxoglobuspropionicus,Bfulgida,Scalinduaspp4,Fe。42:222Scalinduaspp2,KueneniastuttgartiensisBfulgida,56%±8%。Anammoxoglobuspropionicus。4。tem455nm,。Kstuttgartiensis,40%。4,,,。NO2-NH2OH5[7,9,18],NH3NH2OH(HH)N2H4,(HZO)N2H4N2。N2H44CQ,bc1(NR),,1。(HZO)NH3N2。,NO2-。O2,AMO()NH2OH,,。,,。,△P,ATP。5。(11d),fbedac2:aKstuttgartiensis;banammoxoglobuspropionicus;cdBfulgida;efScalinduaspp。(g)Fe。60222Dokhaven()Lichtenvoorde()Olburgen()()/(kg·d-1)4903251200220/(kg·d-1)75015070*220/421262。,,。。,、、、、、、[18-19]。34:1.“*”N2;2.N2。6,,。,。:(1),。(2),,。(3)。(4)。,。、。[][1]MULDERA,vandeGRAAFAA.Anaerobicammoniumoxidationdiscoveredinadenitrifyingfluidized-bedreactor[J].FemsMicrobiolEcol,1995(16):177-183.[2]JETTENMSM,SLICKERSO.Anaerobicammoniumoxidationbymarineandfreshwaterplanctomycete1ikebacteria[J].ApplMicrobiolBiotechnol,2003(63):107-1l4.[3],.[J].,2007,34(1):138-142.[4]JAYNEE,RATTRAYJ,vandeVOSSENBERG.Ladderanelipiddistributioninfourgeneraofanammoxbacteria[J].ArchMicrobiol,2008(190):51-66[5]ANDREWSSC,ROBINSONAK,RODRGQ,etal.Bacterialironhomeostasis[J].FemsMicrobiolRev,2003(27):215-237.[6]KUENENJG,JETTENMSM.Extraordinaryanaerobicammoniumoxidizingbacteria[J].ASMNews,2001(67):456-463.[7]STROUSM,PELLETIERE.Decipheringtheevolutionandmetabolismofananammoxbact[J].JournalofBacteriology,2008,190(2):708-717.[8]vanNIFTRIKL,GEERTSWJC.Combinedstructuralandchemicalanalysisoftheanammoxosome:amembraneboundedintracytoplasmiccompartmentinanammoxbacteria[J].StructBiol,2007(8):115-117.[9]TSUSHIMAI,KINDAICHIT,OKABES.Quantificationofanaerobicammonium-oxidizingbacteriainenrichmentculturesbyreal-timePCR[J].WaterRes,2007(41):785-794.[10]KARTALB,RATTRAYJ.Candidatus''anammoxoglobuspropionicus''anewpropionateoxidizingspeciesofanaerobicammoniumoxidizingbacteriasyst[J].ApplMicrobiol,2006(30):39-49.[11]WINDEYK,DEBOI,VERSTRAETEW.Oxygen-limitedautotrophicnitrificationdenitrification(OLAND)inarotatingbiologicalcontactortreatinghigh-salinitywastewater[J].WaterRes,2005(39):4512-4520.[12]SCHMIDT,WACHTMANNMU.Molecularevidenceforgenusleveldiversityofbacteriacapableofcatalyzinganaerobicammoniumoxidation[J].SystApplMicrobiol,2000(23):93-106.[13]KARTALBJ,RATTRAYLA.Candidatusanammoxoglobuspropionicusanewpropionateoxidizingspeciesofanaerobicammoniumoxidizingbacteria[J].SystApplMicrobiol,2007(30):39-49.[14,.16SrRNA[J].,2008,29(2):469-473.[15]KUYPERSMMM,SLIEKERSAO.AnaerobicammoniumoxidationbyanammoxbacteriaintheBlackSea[J].Nature,2003(422):608-611.[16]SCHMID,WALSHMK.Candidatus“Scalinduabrodae”spNov,Candidatus“Scalinduawagneri”spnov,twonewspeciesofanaerobicammoniumoxidizingbacteria[J].SystApplMicrobiol,2003(26):529-538.[17]SCHMIDMC,RISGAARDPN.Anaerobicammoniumoxidizingbacteriainmarineenvironments:widespreadoccurrencebutlowdiversity[J].EnvironMicrobiol,2007(9):1476-1484.[18],.ABR[J].,2008,21(4):33-35.[19],,,.ABR[J].,2004,17(4):1-2.()61

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