第十章+B细胞介导的体液免疫应答

AntigenrecognitionTcellactivation,proliferationanddifferentiationImmunologicaleffectT细胞免疫应答的基本过程感染部位淋巴器官感染部位上次课IL-12IL-4TGF-β/IL-6IL-2THIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCES流感病毒感染恢复的过程早期阶段以流感病毒滴度的迅速降低为特点，是T细胞依赖的晚期阶段以流感病毒滴度缓慢下降并最终完全清除为特点的，是B细胞依赖的THIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESQ：初次感染流感病毒后第3-5天，即可在血清检测到抗流感病毒的IgM抗体，2周后可以检测到抗流感病毒的IgG，呼吸道黏膜部位可检测到分泌型IgA。请问怎么这些抗体是如何产生的呢？THIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCES第四篇B细胞介导的体液免疫应答Bcellmediatedimmuneresponse何海洋副教授E-mail:[e-mail]基础部全军免疫学研究所THIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESI.B细胞对TD抗原的识别与活化II.B细胞的增殖与分化阶段III.抗体效应IV.体液免疫应答的一般规律V.B细胞对TI抗原的应答(自学）重点难点主要内容本次教学内容THIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESB细胞CD5＋B1细胞CD5－B2细胞针对蛋白质抗原（TD-Ag）应答产生抗体、有免疫记忆针对多糖类抗原（TI-Ag）产生抗体、无免疫记忆B细胞亚群及功能知识回顾参与固有免疫参与适应性免疫THIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCES知识回顾Q:什么是TI-Ag？胸腺非依赖性抗原(thymusindependentantigen,TI-Ag)：其刺激机体产生抗体不需要T细胞辅助，具有丝裂原性质如脂多糖（LPS），或含有多个重复的B细胞表位如细菌荚膜多糖。多个重复的B细胞表位THIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCES知识回顾Q:什么是TD-Ag？胸腺依赖性抗原(thymusdependentantigen,TD-Ag)：其刺激B细胞产生抗体依赖于Th细胞辅助，绝大多数蛋白质抗原属TD-Ag，其既含有B细胞表位，又含有T细胞表位。THIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESQuestion初始B细胞如何遭遇并识别TD-Ag的呢？TD-AgTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESQ:粘膜上皮部位的流感病毒入侵，初始B细胞在何处遭遇流感病毒抗原？初始B细胞在淋巴再循环过程中随时可遭遇抗原粘膜M细胞的转运作用THIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESQ:初始B细胞如何识别抗原？知识回顾识别抗原表位传递抗原信号BCR共受体病原体通过硫酯残基与蛋白质抗原上的半胱氨酸共价结合C3d?知识回顾活化信号放大100倍BCR-Igα/Igβ复合体BCR共受体：CD21/CD19/CD81复合物CD21THIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCES重点内容第二信号(共刺激信号)第一信号(抗原信号):BCR-BcellepitopeQ：CD40L信号由谁提供？CD40-CD40L初始B细胞活化的双信号已活化的Th细胞已经识别抗原的初始B细胞如何活化？THIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESQ:遭遇抗原的初始B细胞在何处接收Th细胞的辅助信号呢？T细胞区初始B细胞遭遇抗原后进入T细胞区重点内容两者必须针对同样的敌人Q:遭遇抗原的B细胞会得到哪些Th细胞辅助并提供第二信号呢？联合识别（linkedrecognition）重要概念初始B细胞和已活化的Th细胞必须分别识别来自同一抗原性物质中的Bcellepitope和Tcellepitope相遇几率:10-8至10-12THIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOFBASICMEDICALSCIENCESTHIRDMILITARYMEDICALUNIVERSITYCOLLEGEOF