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(),2003,25():213216CN53-1045/NISSN0258-7971JournalofYunnanUniversityΞ,(,650091):,1,DNA.:;;;:Q819:A:0258-7971(2003)S-0213-04,DNA,E.Sou2thern1989,,,.,,,,DNA[1,2].,,,[3,4].,[5]..1,,;,,,,[6].:,.;DNA,[7].(1),,2.Affymetrix,5,1,5,.WisconsinGasson,.,1.4cm22000000,40,50000,150000[8].,,4,,.(2),cDNAΞ33(M)(5),,,,:200-0-02:98C018.:197-..cDNA,cDNA,,.2,PCR,.,,,..,[9].,,,,.3,1,,DNA,.,2:,[1012].3.13.1.1DNA,DNA..DNA,,;,[13].663DN13652816.6kb,99%[14].3.1.2,[15].E.A.Winzeler2,3000[16].,,[17].3.1.3[18].,60%P53.AhrendtP53P53P53[19];Hacia96000BRCAL111,15,14[20].3.2,[21].DNA,cDNA[22,23].Girke[24].Derisi,1740[25];Schena245[26];Schena1046cDNAT,5,173[27].,cDNA[28].3.3.HelercDNA,IL-3,Gro-A[29].Buates(S63)[3],412()257108841A.Chee-2840..4(1),.,.(2)P53HIV.,8000cDNA,[31].Affymetrix40000.,,,.(3),,.[31,32].(4),.5,.,,,,..:[1].[J].,1999,19(4):4551.[2]WANGJ.FromDNAbiosensorstogenechips[J].Nu2cleicAcidsResearch,2000,28(16):30113016.[3],.[J].(),2002,(01):711.[][],,()[5]KINGHC,ANIMESHAS.GeneexpressionprofileanalysisbyDNAmicroarrays2promiseandpitfalls[J].JAMA,2001,286:22802288.[6]ZHANGY,PRICEBD,TETRADISS.Reproducibleandinexpensiveproblepreparationforoligonucleotidearrays[J].NuleicAcidsResearch,2001,29(13):66.[7],.[J].,2000,21(9):812814.[8]GASSONSS,GREENRD,YUEY,etal.Masklessfabricationoflight2directedoligonucleotidemicroarraysusingadigitalmicromirrorarray[J].NatureBiotech,1999,17(10):974.[9],.[J].,2000,35(7):57,(8):68.[10],,.[J].,2001,22(06):534537.[11],,.DNA[J].,2002,23(2):128129.[12],.[J].,2000,31(2):135139.[13],,.[J].,2000,5:9598.[14]CHEEM,YANGR,HUBBELE,etal.Accessoingge2neticinformationwithhighdensityarrays[J].Science,1996,274:610614.[15]JACCOUDD,PENGK,FEINSTEIND.Diversityar2rays:asolidstatetechnologyforsequenceinformationindependentgenotyping[J].NucleicAcidsResearch,2001,29(4):25.[16]WINZELEREA,RICHARDSDR,CONWAYAR.Directallelicvariationscanningofthe.yeastgenome[J].Science,1998,281:11941197.[17],.[J].(),2002(01):911.[18]SIHCLAIRA.Gentics101:detectingmutationsinhu2mangenes[J].CMAJ,2002,167(3):275279.[19]AHRENDTSA,HALACHMIS,CHOWJT.Rapidp53sequenceanalysisinprimarylungcancerusinganoligonucleotideprobearray[J].ProcNatlAcadSciUSA,1999,96(13):73827387.[20]HACIAJG,EDGEMONK,CASKEYCT.Twocolorhybridizationanalysisusinghighdensityoligonucleotideassayandenergytransferdyed[J].NucleicAcidRes,1998,26:38653866.[]ITMNTSDNy[]BM,,36655124.J.2000211:9194.21AA.AmicroarrasinmedicalpracticeJ.J200122:111.[22],JIANGYuan,CHENYi2dong.cDNA[J].,1999,44(5):449454.[23],,.MADS[J].,1997,39(11):10351041.[24]GIRKET,TODDJ,RUUSKAS.MicroarrayanalysiaofdevelopingArabidopsisseeds[J].Plantphysiology,2000,124(4):15701581.[25]DERISIJL,LASHKARIDA,MCCUSKERJH.Yeastmicroarraysforgenomewideparallelgeneticandgeneexpressionanalysis[J].ProcNatlAcadSciUSA,1997,94(24):1305713062.[26]SCHENAM,SHALOND,DAVISPO.Quanitativemonitoringofgeneexpressingpatternswithacomple2mentaryDNAmicroarray[J].Science,1995,270:467470.[27]SCHENAM,SHALOND,HELLERR,etal.Parallelhumangenomeanalysis:microarray2basedexpressionmonitoringof1000genes[J].ProcNatlAcadSciUSA,1996,93(20):1061410619.[28]LOCKHARTDH,DONGH,BYRNEMC,etal.Ef2fectsofenvironmentalenrichmentongeneexpressioninthebrain[J].NatureBiotech,1996,14,16751680.[29]HELLERRA,SCHENAM,CHAIA,etal.DiscoveryandanalysisofinflammatorydiseaserelatedgenecD2NAmicroarray[J].ProcNatlAcadSciUSA,1997,94:21502155.[30]BUATESS,MATLASHEWSKIG.Identificationofgenesinducedbyamcrophageactivator,S228453,us2ingexpressionarrayanalysis[J].AntimicrologyAgentsChemother,2001,45(4):11371142.[31]CHCENGLI,WINGHUNGWONG.Model2basedanaly2sisofoligonucleotidearrays:modelvalidation,designis2suesandstandarderrorapplication[J].GenomeBiolo2gy,2001,2(8):00321003211.[32]WORKMANG,JENSENL,JARMERH.AnewnonlinearnormalizationmethodforreducingvariabilityinDNAmicroarrayexpriment[J].GenomeBiology,2002,3(9):00481004816.ThedevelopmentofgenechiptechnologyanditsapplicationGUOZhong2xin,GAOZhi2yong(DepartmentofBiology,YunnanUniversity,Kunming650091,China)Abstract:GenechipisDNAmicroarraythatisfabricatedonsilicawafer,nylonmembrane.Afterhybrid2ingwiththelabeledsample,scanningandanalyzingthechipbycomputer,itcancheckmanytargetsampleatonetime.Genechipispotentiallyusefulforresearchinggeneexpression,discoveringnewgene,diagnosingdis2ease,sequencingDNAandscreeningdrug.Keywords:genechip;genomeproject;geneexpression;genemutation612()25