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##*李莉,陈保锦,谭榜云,刘志武(兰州大学第一医院检验科,兰州730000):对BECKMANLX20和OLYMPUSAU2700全自动生化分析仪部分项目检测结果进行比对和偏差评估参考美国临床和实验室标准化协会文件EP9A,分别在2台仪器上测定新鲜临床混合血清和质控血清,以LX20作为参考仪器,AU2700作为比对仪器,用相关回归分析和配对t检验对相同项目的检测结果进行比对和偏差评估2台分析仪经过比对和校正,大部分项目检测结果有较好的一致性通过对不同生化分析仪测定结果进行比对和偏差评估,有助于验证不同其检测结果间的相关性,有助于仪器评价校正,以满足临床需要:比对研究;偏差;生化分析仪DOI:10.3969/j.issn.1673-4130.2011.12.044:A:1673-4130(2011)12-1356-03,,[1-2],(ClinicalandLaboratoryStandardsInstitute,CLSI)EP9A,2(alanineaminotrans-ferase,ALT)(aspartateaminotrans-ferase,AST)(totalbilirubin,TBIL)(d-irectbilirubin,DBIL)C-(C-glutamyltrans-ferase,GGT)(alkalinephosphatase,ALP)(lactatedehydrogenase,LDH)(creatinekinase,CK)A-(A-hydroxybutyratedehydrogenase,HB-DH)(totalcholesterol,TC)(triacylglyc-erol,TG),2,11.1,,1.2LX20(LX20)(BECKMAN,)AU2700(AU2700,OLYMPUS,)/0(,)Randox(500UN)Randox(518UN)(385UN)1.3(1),;,(2):2,2O(3):20,,LX20(Y),AU2700(X),EP9A,,(r)X:r\0.975r2\0.95X,(b)(a);r0.975()X,ba,[3]AU270020,(4):8,2,2,2h,5d;,(systemicerror%,SE%),CLIAc88,SE%1/2,2[4]1.4Excel,SPSS12.0[5];P0.0522.1220,(coefficientofvariation,CV)(4%),11LX20xsCV(%)AU2700xsCV(%)AST46.321.231.7130.720.721.37ALT46.590.891.2230.931.012.25TBIL48.511.552.2334.161.441.24DBIL26.701.991.3422.300.961.34GGT50.301.172.2132.360.872.68ALP139.071.541.22101.751.010.99LDH170.200.991.13188.182.031.61CK85.660.471.3979.381.081.36HBDH138.302.871.22147.932.701.83TC3.542.561.423.911.041.13TG1.560.690.781.331.221.08*:ASTALTGGTALPLDHCKHBDHU/L,TBILDBILLmol/L,TCTGmmol/L2.22,AST#1356#国际检验医学杂志2011年8月第32卷第12期IntJLabMed,August2011,Vol.32,No.12*:(1010RJZA120)ALTTBILDBILGGTALPLDHHBDH(),(P0.05)AU270032(n=20)rPASTY=0.932X+8.0210.9970.05ALTY=0.843X+6.0580.9920.05TBILY=1.208X-4.7230.9960.05DBILY=1.192X-7.6110.9380.05GGTY=0.954X-3.3600.9940.05ALPY=1.727X+5.8940.9990.05LDHY=1.109X-19.3810.9850.05CKY=0.959X-4.0730.9830.05HBDHY=0.844X+7.5620.9970.05TCY=0.992X+2.0120.9970.05TGY=1.025X+1.0670.9980.05320(n=20)rPASTY=1.021X+0.2100.9990.05ALTY=0.998X+2.5890.9980.05TBILY=1.008X-0.7230.9990.05DBILY=1.092X-0.3110.9980.05GGTY=1.038X-1.1360.9990.05ALPY=1.027X+1.8940.9950.05LDHY=1.009X-2.3810.9970.05CKY=0.930X-0.3730.9960.05HBDHY=0.944X+0.5620.9970.05CHY=0.981X+0.0120.9990.05TGY=0.975X+0.0670.9980.052.34SE%CLIAc881/210%,TBILDBIL4XYSESE%AST20211.005.060611.001.73003033.001.2ALT2017-3.005.260611.001.7300298-2.000.7TBIL23.922.3-1.609.242.743.30.601.4341.9396.554.617.24XYSESE%DBIL6.86.0-0.8011.329.828.61.203.3GGT6058-2.001.81501566.004.5ALP60666.009.240042525.006.4LDH1701766.004.13003088.003.25005066.06.3CK1001022.001.1240237-3.001.318001788-12.001.8HBDH60632.007.9300293-7.003.1500492-8.003.8TC2.322.12-0.204.56.466.29-0.173.310.3610.28-0.081.1TG0.420.41-0.014.82.072.200.136.24.384.920.541.23,2[6-7],(CV3%),,[8]AU2700,ASTALTTBILDBILGGTALPLDHHBDH(P0.05),[9],AU2700,1,,,0.995,,4,TBILDBILSE%1/2CLIAc88,;;;,,,,,,,,,,,[10]:,;;;;#1357#国际检验医学杂志2011年8月第32卷第12期IntJLabMed,August2011,Vol.32,No.12,,,P1/2CLIAc88,,[11],,,,[1]NationalCommitteeforClinicalLaboratoryStandards.EP9-A2MethodofComparisionandBaisEstimationUsingPatientSam-ple[S].Wayne,PA:NCCLS,2002.[2],,,.NCCLSEP9-A22[J].,2006,24(3):169-171.[3],,,.4[J].,2005,23(6):404-407.[4],.[M].:,2001:273.[5],,.[J].,2008,18(8):1666,1678.[6],.[J].,2008,26(3):284-286.[7],,,.276006[J].,2007,25(4):297.[8].t[J].,2001,19(5):303-305.[9],,,.[J].,2003,19(12):1374-1375.[10].[J].,1998,21(1):60-61.[11],,,.[J].,2007,28(9):858-859.(:2011-03-02)##潘莉,王域平,臧钦(河南省信阳市中心医院检验科464000):探讨UF-50尿沉渣分析仪检测尿液红细胞的常见误差原因,分析可减少误差的措施以尿沉渣分析仪与H-500干化学分析对529例尿液标本进行平行检测,分析和探讨尿沉渣分析仪检测红细胞呈假阳性的原因UF-50和H-500检测红细胞阳性例数为128例和107例,阴性为401例和422例;两种方法均为阳性97例,均为阴性391例;阳性符合率为90.65%,阴性符合率为92.65%尿沉渣分析仪与尿干化学分析仪联合应用可有效减少单独检查的局限性,提高尿液红细胞检测准确度,减少误差的发生:红细胞;尿沉渣分析仪;干化学分析DOI:10.3969/j.issn.1673-4130.2011.12.045:A:1673-4130(2011)12-1358-02,,,,11.12010410529,272,247,1~801.2UF-50(UF-50)(Sysmex,),H-500(H-500)H11(,)1.3,2,10mL,1UF-50,1H-500,,2h:400g5min,45b~90b,,0.2mL,50,UF-50,(10@40)10,[1]1.4UF-5025/H-500[2]2529UF-50H-500,90.65%,92.65%,11529UF-50H-500(n)H-500UF-509710107313914221284015293,/0,#1358#国际检验医学杂志2011年8月第32卷第12期IntJLabMed,August2011,Vol.32,No.12