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©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(2342)+0.4%,254nm,0.7ml/min;,,,,0.999;,0.25%2.36%(n=6);93.37%100.6%;0.038mg/L,220.12mg/L,320.011mg/L,420.026mg/L,;;;:R115:B:1004-1257(2008)17-1777-02SubjectDetectionofHippuricAcidandMetylhippuricAcidinUrinebyHighPerformanceLiquidChromatographyAuthorsLULi2ming,HUANGCheng(ZhongshanCentersforDiseaseControlandPrevention,Guangdong,528403,China)Abstract[Objective]ToestablishaHighPerformanceLiquidChromatography(HPLC)methodfordetectinghippuricacid(HA)andmetylhippuricacid(MHA)inurine.[Methods]Methanol+aceticacidsolution(0.4%)wasidentifiedastheflowofmobilephase,thedetectionwavelengthwas254nm,velocitywas0.7ml/min;thesamplesweretreatedwithreversed2phaseHPLCSepara2tion,thequalitativeandthequantitativeanalyseswerecarriedoutbychromatographybasedontheretentiontimeandpeakareare2spectively.[Results]Undertheselectedchromatographyconditions,thelinearrelationshipofstandardcurvewasexcellent,thecorrelationcoefficientswasabove0.999;therelativestandarddeviationwas0.25%2.36%(n=6)intheprecisiontest;Thela2beledrecoveryratewas93.37%100.6%;thelowestlimitofdetectionconcentrationofHA,22MHA,32MHAand42MHAwas0.038,0.12,0.11and0.026mg/Lrespectively.[Conclusion]Themethodissimpleandtheprecisionandaccuracycanmeettherequirementofexperiment,theresultsarereliable.KeywordsHighperformanceliquidchromatography;Urine;HippruicAcid;MethylHippruicAcid(HA)(MHA),,,,HAMHA,WS/T53-1996[1],,HAMHA,[2],,,HAMHA(2342)1111Agilemt(DAD);PALLPURELABClassic;,3000r/minl(=16cm);;;5ml;20=1.19g/ml,20=1.05g/ml,:,,,:,528403;HA,22MHA,32MHA,42MHA,98.0%;;;1.2+0.4%=33+67;:254nm;:0.7ml/min;:351.3WS/T53-19961.4WS/T53-1996,00.10.20.51.0mg/mlHA22MHA,32MHA,42MHA,1.2,10.0l,Agilent11001.510l,1.2,,22.1,HAMHA,0.11.0mg/ml,r0.9998;7771200892417OccupandHealthVol.24No.17Sep2008©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(1.4)0.10.20.5ml,0.10.20.5mg/ml,6,21(min)(mg/L)HA4.67Y=955.92X-2.120.99990.03822MHA8.18Y=306.02X-1.050.99980.12032MHA8.95Y=3325.93X-7.890.99980.01142MHA9.27Y=1381.46X-3.560.99980.0262HAMHA(RSD)(n=6)(mg/ml)(mg/ml)(mg/ml)R3RSD(%)(%)HA0.1840.10.2750.0030.9410-31.0493.230.1630.20.3460.0031.0310-30.570.1020.50.5900.0031.2010-30.2522MHA0.0000.10.1010.0062.2010-32.17100.30.0000.20.2010.0134.3110-32.140.0000.50.4940.0155.8710-31.1932MHA0.0000.10.1020.0072.4110-32.3699.550.0000.20.1940.0031.3310-30.690.0000.50.4980.0270.8010-21.6042MHA0.0000.10.1020.0052.0110-31.97100.10.0000.20.1980.0041.5110-30.760.0000.50.4970.0271.0310-22.07:33pH,,,,,,[3],,=33670.4A,WS/T53-1996=20800.01B(3),AHA22MHA,32MHA,42MHA32HA22MHA32MHA42MHAHA(min)22MHA(min)32MHA(min)42MHA(min)A4.6706.1807.558.95010.109.2700.94B5.0607.3205.1012.2856.7312.2850.00,,,,,4[1]WS/T53-1996..[2],,,..,1995,23(4):449-452.[3]..:,2000:85-88.(:2008-03-11)(:)8771200892417OccupandHealthVol.24No.17Sep2008