三种麻醉药物对小鼠麻醉效果的比较

29420128LABORATORYANIMALSCIENCEVol．29No．4August2012櫴櫴櫴櫴櫴櫴櫴櫴櫴櫴毷毷毷毷116044、、C57BL/6J。C57BL/6J、、。1.2%0.60～0.65mL/20g4%0.20mL/20gC57BL/6J。0.60mL/20g0.20mL/20gC57BL/6J0。C57BL/6JQ95-33A1006－6179201204-0019-062012－07－121983－。。[e-mail]1964－。。[e-mail]1964－。。[e-mail]1。2。、、、。C57BL/6JC57BL。C57BL/6J。3、C57BL/6J。11.11.1.11.2%SIGMAT48402-25G10g10mL2h100%。1.2mL98.8mL1.2%4℃2。1.1.24%CNAB035-Q4g96mL15min。。1.1.320%Q/HGNK204-200020g80mL15min。。1.21。1mL/20g0.5000.5500.6000.6500.7000.1500.1750.2000.2250.2500.1000.1500.200——1.322～25℃。、。1.4C57BL/6J130SCXK2008-290002SYXK2008-000220～30g。22～25℃、50%～70%。1310。1.51.5.1。、、。1.5.2。。1.5.3。1.5.4。1.6ExcelSPSS11.5、、tP0.050.01。22.12.1.10.50mL/20g142s0.60mL/20g、0.65mL/20g0.70mL/20gP＜0.051。P＞0.0587～99s。0.60、0.650.70mL/20g。12.1.20.50mL/20g15min0.65mL/20g0.70mL/20gP＜0.01。0.55～0.65ml/20g40～77minP＞0.052。0.70mL/20g223min。0.55～0.65mL/20g。22.1.30.70mL/20g5h0.55～0.65mL/20g3hP＜0.010.50mL/20g5.5h0.55～0.65mL/20g3。0.55、0.60、0.65mL/20g。、0.60、0.65mL/20gC57BL/6J。0.60mL/20g。32.1.44～60.50mL/20gC57BL/6J、P＜0.05·02·40.70mL/20g、P＜0.05。C57BL/6J0.55、0.60、0.65mL/20g。4562.22.2.1400s、306s、305s、248s251s。0.175、0.200、0225、0.250mL/20gP＞0.05。0.150mL/20g0.225、0.250mL/20gP＜0.0170.150mL/20g。0.200、0.225、0.250mL/20g。2.2.210min、105min、37min、74min228min。0.150mL/20g0.200、0.2250.250mL/20gP＜0.0180.250mL/20g74P＜0.05P＜0.01。0.200mL/20g0.225mL/20g。82.2.30.250mL/20g5.9h0.150mL/20g1.6h、0.175mLL/20g2.5h0.200mL/20g2.1hP＜0.0190.225mL/20g2.2hP＜0.05。0.250mL/20g0.200ml/20g0.225mL/20g。9、·12·290.200～0.225mL/20gC57BL/6J。0.200mL/20g。2.2.410～120.175、0.2000.225mL/20gC57BL/6J、P＜0.050.250mL/20g、。。C57BL/6J0.175mL/20g。1011122.330min。C57BL/6J。2.42。0.7mL/20g0.225mL/20g0.250mL/20g。0.60mL/20g0.20mL/20g。2mL/20g0.5000.5500.6000.6500.700%2000010mL/20g0.1500.1750.2000.2250.250%00030302.5、0.60mL/20g、0.20mL/20g0C57BL/6J。40min37minP＞0.0513。P＜0.01P＜0.05。133、、3。·22·4。。C57BL/6JC57BL/6J。4。C57BL/6J。40min0.60mL/20g。56。4%30%。0.20mL/20gC57BL/6J。。。、C57BL/6J。30min。1500mg/kg。2000mg/kg5min10h90%。2578。C57BL/6J。4。。。。。100%。C57BL/6J15s5～10min。。。4910。C57BL/6J。1.2%0.60mL/20g4%0.20mL/20gC57BL/6J0C57BL/6JC57BL/6J。1．J2009292130～131．2．J2008143264～268．3．J．2007176348～350．4MargareteAPeterAAndreasRetal．OptimizationofIntraperitonealInjectionAnesthesiainMiceDrugsDosagesAdverseEffectsandAnesthesiaDepthJ．ComparativeMedicine2001515443～456．5．J．200825241～43．6．SDJ2008272299～302．7．J．201012111959～1962．8．J．2009279109～110．9SandraBThomasEMJoanneTWetal．SafetyandEfficacyofVariousCombinationsofInjectableAnestheticsinBALB/cMiceJ．JournaloftheAmericanforLaboratoryAnimalScience200847111～17．·32·2910SumikoKAWAIYasuhiroTAKAGIShiroKANEKOetal．EffectofThreeTypesofMixedAnestheticAgentsAlternatetoKetamineinMiceJ．Tokyoexperimentalanimals2011605481～487．AnestheticEffectsofThreeKindofNarcoticDrugsonMiceZHANHong-weiQIUZe-wenLIHui-lingDONGJian-yiWANGAi-guoWANGFu-jinWANGJing-yuLaboratoryAnimalCenterofDalianMedicalUniversityDalian116044ChinaAbstractObjectiveTofindasuitableanesthesiaforC57BL/6Jmicetheanesthesiaeffectsoftribromoethanolchloralhydrateandurethanewerecompared．MethodThethreeanaestheticwereprocessedintraperitonealinjectionwithdifferentdosegradientsrespectivelyandthentheanesthesiaonsettimedepthdurationandrecoverytimewerecompared．ResultTribromoethanol1.2%atdosageof0.60～0.65mL/20gandchloralhydrate4%atdosageof0.20mL/20gshowedthebestanesthesiaeffect．ButtheurethanewasnotsuitableforanaesthesiaofC57BL/6Jmice．ConclusionForanaesthesiaofC57BL/6Jmicetribromoethanol0.60mL/20gandchloralhydrate0.20mL/20ghaveshortestanesthesiaonsettimemoderateanesthesiadurationadequateanesthesiadepthandzeromortalityratebyintraperitonealinjection．KeywordsC57BL/6Janesthesiatribromoethanolchloralhydrate檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶urethane《》、《》201211journalx。“”。201211、、、。、《》·42·