pmd-19t-simple说明书

TakaraCodeD104ApMD®19-TSimpleVector()11111pMD®19-TSimpleVector12ControlDNA2DNA2344Q&A5pMD®19-TSimpleVectorPCRTACloningpUC19pUC19pUC19EcoRVEcoRV3‘TDNAPCRPCR3’APCRLacZ-PCR-PCRPCRPCRDNATpUC19pUC19SolutionI30ControlInsert500bpControlpMD®18-TSimpleVector-pMD®19-TSimpleVector50ng/l20l1ControlInsert50ng/l10l1SolutionI*75l2**-20ControlInsert90%InsertDNATAPCRPCRBcaBESTTMSequencingPrimersM13PrimersDNApMD®19-TSimpleVectorpMD®19-TSimpleVectorCloningsite425BcaBESTSequencingPrimerM13-47bindingsite352~375BcaBESTSequencingPrimerRV-Mbindingsite484~507LacZoperator146~475ColE1ori873~1461Ampr1632~2492-1-ControlDNAA1DNA5lpMD®19-TSimpleVector*11lControlInsert*21ldH2O3l25lSolutionI31630255410l100lJM109305424516890lSOC37607X-GalIPTGAmpL-8PCRBControlInsert/1.52108cfu/gpUC19DNAControlInsert/cfu/gVector%%*2.410696.4902.110546.10*DNAInsert-2-pMD®19-TSimpleVector(2,692bp)DNA1DNA5lpMD®19-TSimpleVector*11lInsertDNA*30.1pmol0.3pmoldH2Oupto5l25lSolutionI31630255PCR2kbDNA410l100lJM109305424516890lSOC37607X-GalIPTGAmpL-8PCR*41DNA5lpMD®19-TSimpleVector*11lInsertDNA*30.1pmol0.3pmoldH2Oupto5l25lSolutionI31630255PCR2kbDNA42l10ngVectorDNAmicrocentrifugetubes2min550lE.coliJM109CompetentCellmicrocentrifugetubes5min6X-GalIPTGAmpL-3730*1pMD®19-TSimpleVector0.5lTpMD®19-TSimpleVector1l50ng0.03pmol*2ControlInsertControlInsert500bp3APCRControlInsert1l50ng0.15pmol*3InsertDNAVectorDNAInsertDNA1210*4DNA-3-1.1108cfu/gpUC19F’LacZM15FragmentDNALacZ--2.InsertDNAInsertDNATakaraTakaraCodeD301D823A3.InsertDNAVectorDNAInsertDNA1210VectorDNAInsertDNAInsertDNAInsertDNAng=nmol660InsertDNAbp1l50ng0.03pmol4.DNApMD®19-TSimpleVector-X-GalIPTGAmpL-DNALacZ2kbDNAPCRBcaBESTTMSequencingPrimersPCR5.ControlInsert106.0.1ngpUC19PlasmidDNA100l900lSOC0.1ngDNA/ml100.01ngDNA/ml100l0.001ngDNA/100l200=200cfu/0.001ng=2105cfu/ng=2108cfu/gpUC19DNA1.PCRPCRDNA2.SolutionI3.InsertDNAPCRPCRDNATA4.20lDNADNATakaraCodeD605ADNA5.2526DNA6.pUC19pUC19JM109-4-Q&AQ-1A-11.InsertDNA3’ADNATakaraPyrobestKODPfxPfuPCRTA2.PCRPCRPCRTakaraTakaraCodeD301D823A3.108cfu/gpUC19DNA4.DNADNADNA5.DNADNA6.SolutionI7.Q-2DNAA-2DNA500bpLacZQ-3DNAA-3pUC19pUC19Q-4DNAA-4PCRPCRDNAQ-5pMD®19-TSimpleVectorpMD®18-TSimpleVectorA-5pMD®19-TSimpleVectorpMD®18-TSimpleVector-pMD®18-TSimpleVector37pMD®19-TSimpleVector1037pMD®19-TSimpleVectorpMD®18-TSimpleVector-5-NoteThisproductisforresearchuseonly.Itisnotintendedforuseintherapeuticordiagnosticproceduresforhumansoranimals.Also,donotusethisproductasfood,cosmetic,orhouseholditem,etc.Takaraproductsmaynotberesoldortransfered,modifiedforresaleortransfer,orusedtomanufacturecommercialproductswithoutwrittenapprovalfromTakaraBiotechnology(Dalian)Co.,Ltd..0411-876416858764168640065187604006518769TakaraBiotechnology(Dalian)Co.,Ltd.19116600No.19Dongbei2ndStreet,DevelopmentZone,Dalian,[e-mail]