李一婧,马伟超,葛仲显(,741001):,,,,,:;;;:Q532+2:A:1005-8141(2011)10-0869-02StudyonXylitolFermentationbyUsingofEnzymeorAcidHydrolysisofCornStalkLIYi-jing,MAWei-chao,GEZhong-xian(CollegeofBioscienceandChemistry,TianshuiNormalUniversity,Tianshui741001,China)Abstract:Thecornstalkhydrolysateconsistofxylosewasobtainedbycellulaseordilutedsulfuricacidhydrolysis,andwasfermentedbyCandidatropicalis.Theresultshowedthattherewasahighercontentofxyloseinacidhydrolysate,andthexylitolproductivityandconversionrateofxylosewereslightlyhigherthanenzymehydrolysis,butnostatisticallysignificantdifference.Keywords:xylitol;cornstalk;cellulose;acidhydrolysis:2011-08-11;:2011-09-25:(:090NKCE081);(:TSA0918):(1981-),,,,1(xylitol)l,2,3,4,5,,,,,,,,,[1],,,,,[2],,22.1(Candidatropicalis,:CICC1779),,4[3](PDA):200g20g20g,1000mL;(PDA):200g20g,1000mL;:40mL(125g/L),5mL30g/L50g/L,pH5.5,15min;(247.6U/mL);(),2.2:,(),1020,36%,1%(110),1073h;,50,OD520,200g/L;pH6.0,CaS04,150g/L[4](110)30pH6.03h;50150g/L,500mL100mL,30210r/min24h,6.5g/L();250mL45mL(),5mL,180r/min,3028h[5](3000r/min20min,)5mL,20mL,30min,10%869ResourceDevelopment&Market201127(10)15mL,5min,0.1mol/LNa2S2O3,l%,,,1.0mL0.1mol/LNa2S2O31902mg3:1:y=0.9649x+0.0007,R2=0.98321:HPLC,,81.9%7.7%6.5%3.9%:1%,1,1(mg/mL)1%0.25330.2328:,,2,3df=3+6-2=7t0.05=2.365,tt0.05,P0.05,,,,,2(mL)12345624.625.124.225.525.830.526.326.326.0---3XS2nS2eSx1-x2t25.95004.516363.23161.52000.164726.20000.02003---:,22,1%,231%,,;,,,,,,;,,,:[1],,,.[J].,2002,33(3)117-119.[2],,,.[J].,2010,(12)104-108.[3],.[M].:,2005187-189.[4],.()[J].,2001,(21)295-299.[5],,,.[J].,2005,(39)546-548.870ResourceDevelopment&Market201127(10)