’sManualPEAKSEPARATIONANDANALYSISSOFTWAREPeakFit®FormoreinformationaboutSeaSolveSoftwareInc.products,pleasevisitourwebsiteat:508-872-5100Fax:508-872-5120TheSOFTWAREanddocumentationareprovidedwithRESTRICTEDRIGHTS.Use,duplication,ordisclosurebytheGovernmentissubjecttorestrictionsassetforthinsubdivision(c)(1)(ii)ofTheRightsinTechnicalDataandComputerSoftwareclauseat52.227-7013.Contractor/manufacturerisSeaSolveSoftwareInc.,235WalnutSt.,Suite7Framingham,MA01702.Generalnotice:Otherproductnamesmentionedhereinareusedforidentificationpurposesonlyandmaybetrademarksoftheirrespectivecompanies.PeakFit v4UsersGuideCopyright©2003bySeaSolveSoftwareInc.Allrightsreserved.PrintedintheUnitedStatesofAmerica.Nopartofthispublicationmaybereproduced,storedinaretrievalsystem,ortransmitted,inanyformorbyanymeans,electronic,mechanical,photocopying,recording,orotherwise,withoutthepriorwrittenpermissionofthepublisher.12345678900706050403ISBN81-88341-07-XContentsPeakFitConcepts.........................1HiddenPeaks.............................1-1ResidualsMethodForFindingHiddenPeaks.............1-2SecondDerivativeMethodForFindingHiddenPeaks.........1-3DeconvolutionMethodForFindingHiddenPeaks...........1-4Convolution,Deconvolution,andConvolutionModels........1-5DeconvolvingaSpectralInstrumentResponseFunction.......1-6DeconvolvinganExponentialDetectorResponseFunction......1-7DeconvolvingIntrinsicPeakSkew...................1-8Non-ParametricDigitalFilteringandEnhancement..........1-10DataSmoothingandFiltering....................1-11SectioningandUniformlySpacedX-Values.............1-12BaselineProcessing.........................1-13AutoScan...............................1-14LevelsofPeakPlacement......................1-14Non-LinearPeakFitting.......................1-15QuickStartTour..........................2LoadingPeakFit............................2-1HiddenPeakData...........................2-1ImportingSAMPLE.XLS........................2-1AutomatedFittingUsingResiduals..................2-2AutomatedFittingUsingtheSecondDerivative............2-6AutomatedFittingUsingDeconvolution................2-9ContentsiiiPeakFitCommonElements....................3PeakFitGraphs.............................3-1PeakFitTextListWindows......................3-20ASCIITextEditor...........................3-22FunctionInsertFeature........................3-24EvaluationProcedure.........................3-25DataImportandEntry.......................4FileMenu...............................4-1EditMenu...............................4-1PeakFitImportOptions........................4-2SupportedFileFormats........................4-2ImportingFileData..........................4-3ImportingWorksheetDataIntoPeakFit................4-4ImportingDataFromSigmaPlotFiles.................4-7ImportingAIAChromatographyFiles.................4-7ImportingDataFromdBaseFiles...................4-7ImportingDataFromASCIIFiles...................4-8SingleAndMulti-ColumnASCIIFiles.................4-10DataInterchangeFormat(DIF)Files.................4-11ImportingDataFromTheWindowsClipboard............4-12SavingX-YData...........................4-13ASCIIList..............................4-14ResetAllDefaults..........................4-14PreviousFiles............................4-15DragAndDropFiles.........................4-15ThePeakFitEditor..........................4-16WeightingData...........................4-20ASCIIEditor.............................4-21CopyingDataTableToClipboard...................4-22ToolBar...............................4-23StatusBar..............................4-23ContentsivPreparingData..........................5DataMenu...............................5-1PrepareMenu.............................5-2ComparewithReference........................5-3NewX-YTitles.............................5-4EnterCalculation............................5-5ApplytoX-YTable...........................5-7CancelCalculation...........................5-7ZeroNegativeData..........................5-8AreaNormalize............................5-9CumulativeArea............................5-9Non-ParametricDigitalFilter.....................5-10ClearInactivePoints.........................5-12ClearX-YData............................5-12Section................................5-13Smooth................................5-16FourierDomainEditing........................5-21DeconvolveGaussianIRF......................5-24DeconvolveExponentialIRF.....................5-28ImportandSubtractBaseline....................5-32Inspect2ndDerivative........................5-33Inspect4thDerivative........................5-35InspectFunction(X).........................5-37AutomatedPeakFitting......................6AutoFitBaseline............................6-1AutoFitPeaksOverview........................6-7AutoFitPeaksIResiduals......................6-10AutoFitPeaksIISecondDerivative..................6-20AutoFitPeaksIIIDeconvolut